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    Method for identifying interaction partners using phage display
    1.
    发明申请
    Method for identifying interaction partners using phage display 审中-公开
    使用噬菌体展示识别交互作用伴侣的方法

    公开(公告)号：US20050042598A1

    公开(公告)日：2005-02-24

    申请号：US10496012

    申请日：2002-11-27

    申请人： Oliver Hill ,  Holger Ottleben

    发明人： Oliver Hill ,  Holger Ottleben

    IPC分类号： G01N21/552 ,  C12Q1/70

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N21/553

    摘要： A method for identifying/selecting interaction partners which interact with a target molecule, with the aid of a support to which anchor molecules are applied that form a polymer-free surface to which affinity ligands are covalently bonded, said ligands being brought into contact with viruses that have a plurality of peptides or proteins as interaction partners on their surface is described. The method described guarantees an accumulation of viruses which present specific interaction partners by an optionally cyclic repetition of the selection. Optionally, selected specific interaction partners are expressed recombinantly once the coding nucleotide has been identified. Furthermore, a surface and its use for a phage display method are described.

    摘要翻译： 用于鉴定/选择与靶分子相互作用的相互作用配偶体的方法，借助于施加锚定分子的载体，其形成亲和配体共价键合的无聚合物表面，所述配体与病毒接触 描述了在其表面上具有多个肽或蛋白质作为相互作用伴侣的物质。 所描述的方法保证通过任选地循环重复选择来呈现特定相互作用伴侣的病毒的积累。 一旦编码核苷酸被鉴定，任选地，重组表达选择的特异性相互作用配偶体。 此外，描述了表面及其用于噬菌体展示方法的用途。

    Method for the selection and identification of peptide or protein molecules by means of phage display
    2.
    发明申请
    Method for the selection and identification of peptide or protein molecules by means of phage display 审中-公开
    通过噬菌体展示法选择和鉴定肽或蛋白质分子的方法

    公开(公告)号：US20050014135A1

    公开(公告)日：2005-01-20

    申请号：US10855668

    申请日：2004-05-27

    申请人： Oliver Hill ,  Holger Ottleben ,  Stefan Dickopf ,  Klaus Burkert

    发明人： Oliver Hill ,  Holger Ottleben ,  Stefan Dickopf ,  Klaus Burkert

    IPC分类号： B01L3/00 ,  G01N21/55 ,  C12Q1/70

    CPC分类号： G01N21/553 ,  B01L3/5085 ,  B82Y30/00

    摘要： The invention relates to a method for the selection and identification of at least on representative (interaction partner) from a plurality of peptide or protein molecules, which can specifically interact with at least one representative from a plurality of target molecules, forming a bond. The inventive method comprises the following steps: (a) a virus system consisting of a plurality of viruses, wherein each virus respectively presents at least on representative from the plurality of peptide or protein molecules on the surface thereof, is brought into contact with the plurality of target molecules (ligands) which are immobilized on the surface of a solid phase carrier such that they are position addressable in a two-dimensional grade; (b) unbound viruses removed from the surface thereof; and (c) the interaction partner is identified by detection and determination of the position of the bond between the immobilized ligand and the interaction partner presented by the virus with the aid of a marker-free detection method. The described method makes it possible to concentrate viruses presenting interaction partners by means of an optionally cyclic repetition of selection. Optionally, selected interaction partners are recombinantly expressed after identification of the coding nucleotide sequence.

    摘要翻译： 本发明涉及一种用于从多个肽或蛋白质分子中选择和鉴定至少一个代表性（相互作用配偶体）的方法，其可与多个靶分子的至少一个代表特异性相互作用，形成键。 本发明的方法包括以下步骤：（a）由多种病毒组成的病毒系统，其中每个病毒分别至少表现出其表面上的多个肽或蛋白质分子的代表，与多个病毒接触 的固定在固相载体的表面上的靶分子（配体），使得它们位于二维等级的位置; （b）从其表面去除未结合的病毒; 和（c）通过利用无标记检测方法的检测和确定固定的配体与病毒所呈现的相互作用配偶之间的键的位置来鉴定相互作用配偶体。 所描述的方法使得可以通过可选地循环重复的选择来浓缩呈现相互作用伴侣的病毒。 任选地，在识别编码核苷酸序列后重组表达所选择的相互作用伴侣。