利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.027 秒)

    Method for the selection and identification of peptide or protein molecules by means of phage display
    1.
    发明申请
    Method for the selection and identification of peptide or protein molecules by means of phage display 审中-公开
    通过噬菌体展示法选择和鉴定肽或蛋白质分子的方法

    公开(公告)号：US20050014135A1

    公开(公告)日：2005-01-20

    申请号：US10855668

    申请日：2004-05-27

    申请人： Oliver Hill ,  Holger Ottleben ,  Stefan Dickopf ,  Klaus Burkert

    发明人： Oliver Hill ,  Holger Ottleben ,  Stefan Dickopf ,  Klaus Burkert

    IPC分类号： B01L3/00 ,  G01N21/55 ,  C12Q1/70

    CPC分类号： G01N21/553 ,  B01L3/5085 ,  B82Y30/00

    摘要： The invention relates to a method for the selection and identification of at least on representative (interaction partner) from a plurality of peptide or protein molecules, which can specifically interact with at least one representative from a plurality of target molecules, forming a bond. The inventive method comprises the following steps: (a) a virus system consisting of a plurality of viruses, wherein each virus respectively presents at least on representative from the plurality of peptide or protein molecules on the surface thereof, is brought into contact with the plurality of target molecules (ligands) which are immobilized on the surface of a solid phase carrier such that they are position addressable in a two-dimensional grade; (b) unbound viruses removed from the surface thereof; and (c) the interaction partner is identified by detection and determination of the position of the bond between the immobilized ligand and the interaction partner presented by the virus with the aid of a marker-free detection method. The described method makes it possible to concentrate viruses presenting interaction partners by means of an optionally cyclic repetition of selection. Optionally, selected interaction partners are recombinantly expressed after identification of the coding nucleotide sequence.

    摘要翻译： 本发明涉及一种用于从多个肽或蛋白质分子中选择和鉴定至少一个代表性（相互作用配偶体）的方法，其可与多个靶分子的至少一个代表特异性相互作用，形成键。 本发明的方法包括以下步骤：（a）由多种病毒组成的病毒系统，其中每个病毒分别至少表现出其表面上的多个肽或蛋白质分子的代表，与多个病毒接触 的固定在固相载体的表面上的靶分子（配体），使得它们位于二维等级的位置; （b）从其表面去除未结合的病毒; 和（c）通过利用无标记检测方法的检测和确定固定的配体与病毒所呈现的相互作用配偶之间的键的位置来鉴定相互作用配偶体。 所描述的方法使得可以通过可选地循环重复的选择来浓缩呈现相互作用伴侣的病毒。 任选地，在识别编码核苷酸序列后重组表达所选择的相互作用伴侣。

    Sensor Arrangement
    2.
    发明申请
    Sensor Arrangement 审中-公开
    传感器布置

    公开(公告)号：US20070273883A1

    公开(公告)日：2007-11-29

    申请号：US10564146

    申请日：2004-06-21

    申请人： Stefan Dickopf ,  Klaus Burkert ,  Alexander Maier ,  Kristina Schmidt

    发明人： Stefan Dickopf ,  Klaus Burkert ,  Alexander Maier ,  Kristina Schmidt

    IPC分类号： G01N21/55

    CPC分类号： G01N21/553 ,  G01N21/253 ,  G01N2035/00158 ,  G01N2035/1037 ,  Y10T428/12549

    摘要： The invention relates to a sensor arrangement comprising a radiation-conducting substrate, said arrangement having a plurality of sensor fields and separating regions for separating the individual sensor fields from the respectively adjacent sensor fields. Said separating regions are formed by a separating agent layer that causes a reflectivity lower than 0.5 for radiation from the substrate on the interface between the separating agent layer and the substrate, at least in a first region adjacent to the interface between the separating agent layer and the substrate, and an extinction higher than 0.95 at least in a second region located above the first region, on the side opposing the substrate.

    摘要翻译： 本发明涉及一种包括辐射传导基片的传感器装置，所述装置具有多个传感器场和分离区域，用于将各个传感器场与相邻的传感器场分开。 所述分离区域由分离剂层形成，所述分离剂层至少在与分离剂层和基底之间的界面相邻的第一区域中引起反射率低于0.5，用于来自分离剂层和基底之间的界面上的基底的辐射 衬底，并且至少在位于第一区域上方的第二区域中，在与衬底相对的一侧上高于0.95的消光。

    SPR sensor system
    3.
    发明授权
    SPR sensor system 失效
    SPR传感器系统

    公开(公告)号：US06752963B2

    公开(公告)日：2004-06-22

    申请号：US09789015

    申请日：2001-02-21

    申请人： Stefan Dickopf ,  Kristina Schmidt ,  Dirk Vetter ,  Klaus Burkert

    发明人： Stefan Dickopf ,  Kristina Schmidt ,  Dirk Vetter ,  Klaus Burkert

    IPC分类号： G01N2155

    CPC分类号： G01N21/253 ,  G01N21/553

    摘要： The invention relates to an SPR sensor array comprising a plurality of SPR sensor surface areas (120) arranged on a substrate (10, 20) in a two-dimensional matrix located in a plane to which the SPR sensor surface areas (120) are parallel and whereby radiation capable of exciting surface plasmons in the SPR sensor surface areas (120) under specific physical conditions can be guided through the substrate (10, 20) to be reflected from the SPR sensor surface areas, as well as separating means (110) for separating each SPR sensor surface area (120) from its neighboring SPR sensor surface area (120), wherein the separating means (110) and SPR sensor surface areas (120) are provided such that at least outside of surface plasmon resonance occurring in the SPR sensor surface areas (120) the radiation (40) guided through the substrate (10, 20) is reflected in the region of the separating means to a different degree than in the region of the SPR sensor surface areas (120) to create a contrast between the separating means (110) and the SPR sensor surface areas (120) at least outside of surface plasmon resonance occurring in the SPR sensor surface areas (120) in the radiation reflected by the SPR sensor surface areas (120) and the separating means (110).

    摘要翻译： 本发明涉及一种SPR传感器阵列，其包括布置在位于SPR传感器表面区域（120）平行的平面中的二维矩阵中的衬底（10,20）上的多个SPR传感器表面区域（120） 并且由此能够在特定物理条件下激发SPR传感器表面区域（120）中的表面等离子体激元的辐射能够被引导穿过衬底（10,20）以从SPR传感器表面区域反射，以及分离装置（110） 用于将每个SPR传感器表面区域（120）与其相邻的SPR传感器表面区域（120）分离，其中分离装置（110）和SPR传感器表面区域（120）被设置成使得至少在表面等离子体共振 引导穿过衬底（10,20）的辐射（40）的SPR传感器表面区域（120）在分离装置的区域中被反射到与SPR传感器表面区域（120）的区域不同的程度，以产生 对比是 在由SPR传感器表面区域（120）和分离装置（120）反射的辐射中至少在出现在SPR传感器表面区域（120）中的表面等离子体共振之外的分离装置（110）和SPR传感器表面区域（120） （110）。