公开(公告)号：US08551917B2

公开(公告)日：2013-10-08

申请号：US11269417

申请日：2005-11-07

申请人： Pat J. Unkefer ,  Rodolfo A. Martinez ,  Thomas J. Knight

发明人： Pat J. Unkefer ,  Rodolfo A. Martinez ,  Thomas J. Knight

IPC分类号： A01N43/36 ,  A61K9/00

CPC分类号： A01N43/36 ,  A01N37/38

摘要： The present invention describes a composition including a mixture of L- and D-pyroglutamate stereoisomers in a ratio of L to D of from about 80:20 to about 97:3, and, a carrier medium for application of the L- and D-pyroglutamate stereoisomers to a target plant. The composition can also be used as a germination medium and may be incorporated into a seed coat for assisting in germination. The present invention further describes a method of increasing the agronomic performance of a target plant by treating a target plant with a composition including a mixture of L- and D-pyroglutamate stereoisomers in a ratio of L to D of from about 80:20 to about 97:3 and a carrier medium for said L- and D-pyroglutamate stereoisomers.

摘要翻译： 本发明描述了一种组合物，其包含L至D的比例为约80:20至约97:3的L-和D-焦谷氨酸盐立体异构体的混合物，以及用于施用L-和D- 焦谷氨酸立体异构体。 组合物也可以用作发芽培养基，并且可以并入种皮中以辅助发芽。 本发明进一步描述了通过用包含L-和D-焦谷氨酸钠立体异构体的混合物的组合物处理靶植物，其比例为约80:20至约 97：3和用于所述L-和D-焦谷氨酸立体异构体的载体介质。

公开(公告)号：US06555500B1

公开(公告)日：2003-04-29

申请号：US10124520

申请日：2002-04-16

申请人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

发明人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

IPC分类号： A01N4336

CPC分类号： A01N43/36 ,  Y02P60/246

摘要： Increasing the concentration of prolines such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that overexpress glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramnate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

摘要翻译： 已经显示在植物叶面部分增加脯氨酸如2-羟基-5-氧代脯氨酸的浓度导致二氧化碳固定，生长速率，干重，营养价值（氨基酸），结瘤和固氮的增加 ，光合作用衍生的化学能，以及对野生型植物具有相同性质的抗病虫害。 这可以通过四种方式实现：（1）通过喷洒这些部分将脯氨酸溶液直接施加到植物的叶面部分; （2）将脯氨酸溶液施用于植物根部; （3）对植物进行遗传工程和筛选，以产生过度表达叶中谷氨酰胺合成酶的产生线，从而导致代谢物浓度升高，2-羟基-5-氧代脯氨酸（该脯氨酸也称为2-氧代戊二酸）。 （4）损害植物根系中的谷氨酰胺合成酶活性，导致叶中谷氨酰胺合成酶活性升高，导致2-羟基-5-氧代脯氨酸浓度增加。 脯氨酸也被发现在藻类中诱发类似的作用。

公开(公告)号：US06831040B1

公开(公告)日：2004-12-14

申请号：US09493039

申请日：2000-01-27

申请人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

发明人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

IPC分类号： A01N4336

CPC分类号： A01N43/36 ,  Y02P60/246

摘要： Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

摘要翻译： 植物叶片中增加脯氨酸浓度，如2-羟基-5-氧代脯氨酸，已被证明导致二氧化碳固定，生长速率，干重，营养价值（氨基酸），结瘤和氮素的增加 固定，光合作用衍生的化学能，以及对野生型植物具有相同性质的抗病虫害。 这可以通过四种方式实现：（1）通过喷洒这些部分将脯氨酸溶液直接施加到植物的叶面部分; （2）将脯氨酸溶液施用于植物根部; （3）对植物进行遗传工程和筛选，以产生过量表达叶中谷氨酰胺合成酶的产生线，从而导致代谢物浓度增加，2-羟基-5-氧代脯氨酸（该脯氨酸也称为2-氧代谷氨酸）。 （4）损害植物根系中的谷氨酰胺合成酶活性，导致叶中谷氨酰胺合成酶活性升高，导致2-羟基-5-氧代脯氨酸浓度增加。 脯氨酸也被发现在藻类中诱发类似的作用。

公开(公告)号：US06593275B1

公开(公告)日：2003-07-15

申请号：US10124568

申请日：2002-04-16

申请人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

发明人： Pat J. Unkefer ,  Thomas J. Knight ,  Rodolfo A. Martinez

IPC分类号： A01N4336

CPC分类号： A01N43/36 ,  Y02P60/246

摘要： Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

摘要翻译： 植物叶片中增加脯氨酸浓度，如2-羟基-5-氧代脯氨酸，已被证明导致二氧化碳固定，生长速率，干重，营养价值（氨基酸），结瘤和氮素的增加 固定，光合作用衍生的化学能，以及对野生型植物具有相同性质的抗病虫害。 这可以通过四种方式实现：（1）通过喷洒这些部分将脯氨酸溶液直接施加到植物的叶面部分; （2）将脯氨酸溶液施用于植物根部; （3）对植物进行遗传工程和筛选，以产生过量表达叶中谷氨酰胺合成酶的产生线，从而导致代谢物浓度增加，2-羟基-5-氧代脯氨酸（该脯氨酸也称为2-氧代谷氨酸）。 （4）损害植物根系中的谷氨酰胺合成酶活性，导致叶中谷氨酰胺合成酶活性升高，导致2-羟基-5-氧代脯氨酸浓度增加。 脯氨酸也被发现在藻类中诱发类似的作用。

公开(公告)号：US06288240B1

公开(公告)日：2001-09-11

申请号：US09584621

申请日：2000-05-31

申请人： Rodolfo A. Martinez ,  Pat J. Unkefer

发明人： Rodolfo A. Martinez ,  Pat J. Unkefer

IPC分类号： C07D20728

CPC分类号： C07D207/277

摘要： The compound 2-hydroxy-5-oxoproline and analogs thereof may be used to produce an increase in carbon dioxide fixation, growth, dry weight, nutritional value (proteins and amino acids), nodulation and nitrogen fixation and photosynthetically derived chemical energy when applied to plants through their roots and/or through their foliar portions. The present invention includes an essentially quantitative chemical synthesis for this compound which is performed in a single step reaction of Fremy's Salt (potassium nitrosodisulphonate) with either glutamine or 2-pyrrolidone-5-carboxylic acid. Fremy's salt (potassium nitrosodisulphonate) is available commercially, or can be readily synthesized.

摘要翻译： 化合物2-羟基-5-氧代脯氨酸及其类似物可用于在应用于二氧化碳固定，生长，干重，营养价值（蛋白质和氨基酸），结瘤和固氮以及光合作用衍生的化学能时产生增加 植物通过其根部和/或通过它们的叶面部分。 本发明包括在Fremy's Salt（亚硝基二磺酸钾）与谷氨酰胺或2-吡咯烷酮-5-羧酸的单步反应中进行的该化合物的基本上定量的化学合成。 Fremy's盐（亚硝基二磺酸钾）可商购，也可以容易地合成。

公开(公告)号：US20110011266A1

公开(公告)日：2011-01-20

申请号：US12504917

申请日：2009-07-17

申请人： Merritt C. Helvenston ,  Rodolfo A. Martinez ,  Jose C' De Baca ,  John R. Juares

发明人： Merritt C. Helvenston ,  Rodolfo A. Martinez ,  Jose C' De Baca ,  John R. Juares

IPC分类号： B01D53/18

CPC分类号： B08B1/00

摘要： An air filtration system and method for the remediation of hydrazine from contaminated air. The system is provided in the form of a container with an inlet port and an outlet port containing absorbent pads or filters or wipes saturated with a solution of a dicarbonyl compound. As contaminated air passes through the container, hydrazine present within the air reacts with the dicarbonyl compound and becomes neutralized, thereby resulting in the exit of pure air from the container. The absorbent pads can also be used independently to remove hydrazine liquid spill.

摘要翻译： 一种用于从污染空气中修复肼的空气过滤系统和方法。 该系统以具有入口端口和出口端口的形式提供，该入口端口和出口端口包含用二羰基化合物溶液饱和的吸收垫或过滤器或擦拭物。 当受污染的空气通过容器时，存在于空气中的肼与二羰基化合物反应并中和，从而导致纯空气从容器中排出。 吸收垫也可以独立使用以除去肼液体溢出物。

公开(公告)号：US07662993B2

公开(公告)日：2010-02-16

申请号：US11591828

申请日：2006-11-02

申请人： Marc A. Alvarez ,  Rodolfo A. Martinez ,  Clifford J. Unkefer

发明人： Marc A. Alvarez ,  Rodolfo A. Martinez ,  Clifford J. Unkefer

IPC分类号： C07C317/12 ,  C07C321/22

CPC分类号： C07C51/377 ,  C07B2200/05 ,  C07C67/317 ,  C07C317/44 ,  C07C323/52 ,  C07C57/04 ,  C07C69/54

摘要： The present invention is directed to labeled compounds of the formulae wherein Q is selected from the group consisting of —S(═O)—, and —S(═O)2—, Z is selected from the group consisting of 1-naphthyl, substituted 1-naphthyl, 2-naphthyl, substituted 2-naphthyl, and phenyl groups with the structure wherein R1, R2, R3, R4 and R5 are each independently selected from the group consisting of hydrogen, a C1-C4 lower alkyl, a halogen, and an amino group selected from the group consisting of NH2, NHR and NRR′ where R and R′ are each independently selected from the group consisting of a C1-C4 lower alkyl, an aryl, and an alkoxy group, and X is selected from the group consisting of hydrogen, a C1-C4 lower alkyl group, and a fully-deuterated C1-C4 lower alkyl group.

摘要翻译： 本发明涉及下式的标记化合物其中Q选自-S（ - ） - 和-S（ - O）2 - ，Z选自1-萘基， 取代的1-萘基，2-萘基，取代的2-萘基和具有以下结构的苯基：其中R 1，R 2，R 3，R 4和R 5各自独立地选自氢，C 1 -C 4低级烷基，卤素 和选自NH 2，NHR和NRR'的氨基，其中R和R'各自独立地选自C 1 -C 4低级烷基，芳基和烷氧基，并且X选自 由氢，C1-C4低级烷基和完全氘化的C1-C4低级烷基组成的组。

公开(公告)号：US20090259064A1

公开(公告)日：2009-10-15

申请号：US12421458

申请日：2009-04-09

申请人： Rodolfo A. Martinez

发明人： Rodolfo A. Martinez

IPC分类号： C07F9/40 ,  C07C317/14 ,  C07C381/00

CPC分类号： C07C317/14 ,  C07B2200/05 ,  C07C321/28 ,  C07F9/4006

摘要： High purity isotopically labeled phosphonic acid esters can be obtained from isotopically enriched Chloro[13C]methyl phenyl sulfide The labeled phosphonic acid esters can then be used as precursors for the one step production of labeled vinyl sulfides, labeled vinyl sulfoxides, and labeled vinyl sulfones. The labeled phosphonic acid esters can also be reacted with a variety of aldehydes to produce extended vinyl systems that are isotopically labeled.

摘要翻译： 高纯度同位素标记的膦酸酯可以从同位素富集的氯[13 C]甲基苯基硫化物获得。然后，标记的膦酸酯可用作标记乙烯基硫醚标记的乙烯基亚砜和标记乙烯基砜一步法生产的前体。 标记的膦酸酯也可以与各种醛反应以产生同位素标记的延伸的乙烯基体系。

公开(公告)号：US07582801B2

公开(公告)日：2009-09-01

申请号：US11052589

申请日：2005-02-07

申请人： Rodolfo A. Martinez ,  Clifford J. Unkefer ,  Marc A. Alvarez

发明人： Rodolfo A. Martinez ,  Clifford J. Unkefer ,  Marc A. Alvarez

IPC分类号： C07C49/04

CPC分类号： C07C69/716 ,  C07B59/001 ,  C07B2200/05 ,  C07C59/347 ,  C07C235/80 ,  C07C317/24 ,  C07C317/46 ,  Y10T436/200833

摘要： The present invention is directed to labeled compounds, of the formulae wherein C* is each independently selected from the group consisting of 13C and 12C with the proviso that at least one C* is 13C, each hydrogen of the methylene group can independently be either hydrogen or deuterium, the methyl group includes either zero or three deuterium atoms, Q is from the group of sulfide, sulfinyl, and sulfone, Z is an aryl group from the group of 1-naphthyl, substituted 1-naphthyl, 2-naphthyl, substituted 2-naphthyl, and phenyl groups with the structure wherein R1, R2, R3, R4 and R5 are each independently from the group of hydrogen, a C1-C4 lower alkyl, a halogen, and an amino group from the group of NH2, NHR and NRR′ where R and R′ are each independently from the group of a C1-C4 lower alkyl, a phenyl, and an alkoxy group, and the methyl group can include either zero or three deuterium atoms.

摘要翻译： 本发明涉及下式的标记化合物，其中C *各自独立地选自13 C和12 C，条件是至少一个C *为13 C，亚甲基的每个氢可以独立地为氢 或氘，甲基包括零或三个氘原子，Q为硫醚，亚磺酰基和砜基，Z为1-萘基，取代的1-萘基，2-萘基，取代的芳基 2-萘基和具有其中R 1，R 2，R 3，R 4和R 5各自独立地为氢，C 1 -C 4低级烷基，卤素和氨基的基团的苯基，其中NH 2，NHR 和NRR'，其中R和R'各自独立地为C1-C4低级烷基，苯基和烷氧基的基团，甲基可以包括零或三个氘原子。

公开(公告)号：US06764673B2

公开(公告)日：2004-07-20

申请号：US10342521

申请日：2003-01-14

申请人： Rodolfo A. Martinez ,  Marc A. Alvarez ,  Louis A. Silks, III ,  Clifford J. Unkefer ,  Jurgen G. Schmidt

发明人： Rodolfo A. Martinez ,  Marc A. Alvarez ,  Louis A. Silks, III ,  Clifford J. Unkefer ,  Jurgen G. Schmidt

IPC分类号： A61K5100

CPC分类号： C07C321/28 ,  C07B2200/05 ,  C07C317/14

摘要： The present invention is directed to labeled compounds, [2H1, 13C], [2H2, 13C] and [2H3, 13C]methyl aryl sulfones and [2H1, 13C], [2H2, 13C] and [2H3, 13C]methyl aryl sulfoxides, wherein the 13C methyl group attached to the sulfur of the sulfone or sulfoxide includes exactly one, two or three deuterium atoms and the aryl group is selected from the group consisting of 1-naphthyl, substituted 1-naphthyl, 2-naphthyl, substituted 2-naphthyl, and phenyl groups with the structure: wherein R1, R2, R3, R4 and R5 are each independently, hydrogen, a C1-C4 lower alkyl, a halogen, an amino group from the group consisting of NH2, NHR and NRR′ where R and R′ are each a C1-C4 lower alkyl, a phenyl, or an alkoxy group. The present invention is also directed to processes of preparing methyl aryl sulfones and methyl aryl sulfoxides.

摘要翻译： 本发明涉及标记的化合物，[2 H 1，C 13 C]，[2 H 2，13 C]和[2 H 3，13 C]甲基芳基砜和[ 2> H1，13 C]，[2 H 2，13 C]和[2 H 3，13 C]甲基芳基亚砜，其中与硫连接的13 C甲基 砜或亚砜包括恰好一个，两个或三个氘原子，并且芳基选自1-萘基，取代的1-萘基，2-萘基，取代的2-萘基和具有以下结构的苯基：其中 R 1，R 2，R 3，R 4和R 5各自独立地为氢，C 1 -C 4低级烷基，卤素，由NH 2，NHR和NRR'组成的组中的氨基，其中R和R'各自为C 1 -C 4 低级烷基，苯基或烷氧基。 本发明还涉及制备甲基芳基砜和甲基芳基亚砜的方法。