Glycosyltransferase gene gtfC from Amycolatopsis orientalis
    1.
    发明授权
    Glycosyltransferase gene gtfC from Amycolatopsis orientalis 失效
    来自双孢杆菌的糖基转移酶基因gtfC

    公开(公告)号:US6027928A

    公开(公告)日:2000-02-22

    申请号:US120249

    申请日:1998-07-21

    CPC分类号: C12N9/1048 Y10S435/911

    摘要: The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfC of Amycolatopsis orientalis. Also provided are vectors carrying the gtfC gene, transformed heterologous host cells for expressing the GtfC protein, and methods for producing glycopeptide compounds using the cloned gtfC gene.

    摘要翻译: 本发明提供了编码双歧杆菌的糖基转移酶蛋白GtfC的分离的核酸化合物。 还提供了携带gtfC基因的载体,用于表达GtfC蛋白的转化的异源宿主细胞,以及使用克隆的gtfC基因产生糖肽化合物的方法。

    Method of selection for spiramycin resistance in streptomyces
    2.
    发明授权
    Method of selection for spiramycin resistance in streptomyces 失效
    在链霉菌中选择螺旋霉素抗性的方法

    公开(公告)号:US4753880A

    公开(公告)日:1988-06-28

    申请号:US685677

    申请日:1984-12-24

    摘要: A novel method of selecting Streptomyces recombinant DNA-containing host cells and vectors useful in the method are described. The vectors confer spiramycin resistance to sensitive Streptomyces host cells and thus provide a convenient method of selecting Streptomyces transformants. The novel spiramycin resistance-conferring gene described can be isolated on an .about.3.8 kb Bc1I restriction fragment from plasmid pNAS105. Plasmid pNAS105 can be isolated from Streptomyces griseofuscus C581/pNAS105 (NRRL 15919).

    摘要翻译: 描述了选择含有重链DNA的宿主细胞的新方法和用于该方法的载体。 载体赋予敏感链霉菌宿主细胞的螺旋霉素抗性,从而提供选择链霉菌转化体的方便方法。 所述的新型螺旋霉素抗性基因基因可以在质粒pNAS105的差异3.8kb Bc1I限制性片段上分离。 质粒pNAS105可以从灰色链霉菌C581 / pNAS105(NRRL 15919)中分离。

    Glycosyltransferase gene GFTD from amycolatopsis orientalis
    5.
    发明授权
    Glycosyltransferase gene GFTD from amycolatopsis orientalis 失效
    来自无性繁殖体的糖基转移酶基因GFTD

    公开(公告)号:US6025174A

    公开(公告)日:2000-02-15

    申请号:US120052

    申请日:1998-07-21

    IPC分类号: C12N1/21 C12N9/10 C12N15/54

    CPC分类号: C12N9/1051

    摘要: The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfD of Amycolatopsis orientalis. Also provided are vectors carrying the gtfD gene, transformed heterologous host cells for expressing the GtfD protein, and methods for producing glycopeptide compounds using the cloned gtfD gene.

    摘要翻译: 本发明提供了编码无性繁殖体的糖基转移酶蛋白GtfD的分离的核酸化合物。 还提供携带gtfD基因的载体,用于表达GtfD蛋白质的转化的异源宿主细胞,以及使用克隆的gtfD基因产生糖肽化合物的方法。

    Glucosltransferase gene GTFE from amycolatopsis orientalis
    6.
    发明授权
    Glucosltransferase gene GTFE from amycolatopsis orientalis 失效
    来自无性繁殖体的葡萄糖转移酶基因GTFE

    公开(公告)号:US5932464A

    公开(公告)日:1999-08-03

    申请号:US120053

    申请日:1998-07-21

    IPC分类号: C12N1/21 C12N9/10

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode the enzyme, transformed heterologous host cells for expressing the enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode the enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码酶的基因的载体,用于表达酶的转化的异源宿主细胞,以及使用编码该酶的克隆基因产生糖肽化合物的方法。

    Glycosyltransferase gene gtfD from amycolatopsis orientalis
    7.
    发明授权
    Glycosyltransferase gene gtfD from amycolatopsis orientalis 失效
    来自无性繁殖体的糖基转移酶基因gtfD

    公开(公告)号:US5821098A

    公开(公告)日:1998-10-13

    申请号:US924847

    申请日:1997-09-05

    CPC分类号: C12N9/1051

    摘要: The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfD of Amycolatopsis orientalis. Also provided are vectors carrying the gtfD gene, transformed heterologous host cells for expressing the GtfD protein, and methods for producing glycopeptide compounds using the cloned gtfD gene.

    摘要翻译: 本发明提供了编码无性繁殖体的糖基转移酶蛋白GtfD的分离的核酸化合物。 还提供携带gtfD基因的载体,用于表达GtfD蛋白质的转化的异源宿主细胞,以及使用克隆的gtfD基因产生糖肽化合物的方法。

    Method for isolating transposable elements from streptomyces
    8.
    发明授权
    Method for isolating transposable elements from streptomyces 失效
    从链霉菌中分离转座因子的方法

    公开(公告)号:US5264354A

    公开(公告)日:1993-11-23

    申请号:US898353

    申请日:1992-06-12

    IPC分类号: C12N1/21 C12N15/00 C12N15/76

    CPC分类号: C12N15/76 C12N15/00

    摘要: A novel method for isolating transposable elements was used to isolate an approximately 1.6 kb insertion sequence from Streptomyces. The method entails transforming a cell with a plasmid containing a repressor gene, so that the introduction of a transposable element into the gene allows the expression of a selectable marker in a second host cell. The novel insertion sequence isolated from Streptomyces lividans CT2 has been designated IS493.

    摘要翻译: 用于分离转座元件的新方法用于从Streptomyces中分离出大约1.6kb的插入序列。 该方法需要用含有阻遏物基因的质粒转化细胞,从而将可转座元件引入基因允许在第二宿主细胞中表达选择性标记。 从链霉菌(Streptomyces lividans)CT2分离的新插入序列已经被命名为IS493。

    Glycopeptides produced using Amycolatopsis orientalis glycosyltransferase gtfE
    9.
    发明授权
    Glycopeptides produced using Amycolatopsis orientalis glycosyltransferase gtfE 失效
    使用双歧杆菌(Asycolatopsis orientalis)糖基转移酶gtfE产生的糖肽

    公开(公告)号:US07439322B2

    公开(公告)日:2008-10-21

    申请号:US10401271

    申请日:2003-03-27

    IPC分类号: A61K38/14 C07K9/00

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码所述酶的基因的载体,用于表达所述酶的转化的异源宿主细胞,以及使用编码所述酶的克隆基因产生糖肽化合物的方法。

    Glucosyltransferase gene gtfe from Amycolatopsis orientalis
    10.
    发明授权
    Glucosyltransferase gene gtfe from Amycolatopsis orientalis 有权
    葡萄糖转移酶基因,

    公开(公告)号:US06232102B1

    公开(公告)日:2001-05-15

    申请号:US09198212

    申请日:1998-11-23

    IPC分类号: C12P1944

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码所述酶的基因的载体,用于表达所述酶的转化的异源宿主细胞,以及使用编码所述酶的克隆基因产生糖肽化合物的方法。