Glycopeptides produced using Amycolatopsis orientalis glycosyltransferase gtfE
    1.
    发明授权
    Glycopeptides produced using Amycolatopsis orientalis glycosyltransferase gtfE 失效
    使用双歧杆菌(Asycolatopsis orientalis)糖基转移酶gtfE产生的糖肽

    公开(公告)号:US07439322B2

    公开(公告)日:2008-10-21

    申请号:US10401271

    申请日:2003-03-27

    IPC分类号: A61K38/14 C07K9/00

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码所述酶的基因的载体,用于表达所述酶的转化的异源宿主细胞,以及使用编码所述酶的克隆基因产生糖肽化合物的方法。

    Glucosyltransferase gene gtfe from Amycolatopsis orientalis
    2.
    发明授权
    Glucosyltransferase gene gtfe from Amycolatopsis orientalis 有权
    葡萄糖转移酶基因,

    公开(公告)号:US06232102B1

    公开(公告)日:2001-05-15

    申请号:US09198212

    申请日:1998-11-23

    IPC分类号: C12P1944

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码所述酶的基因的载体,用于表达所述酶的转化的异源宿主细胞,以及使用编码所述酶的克隆基因产生糖肽化合物的方法。

    Glycosyltransferase gene gtfE from Amycolatopsis oreintalis
    4.
    发明授权
    Glycosyltransferase gene gtfE from Amycolatopsis oreintalis 失效
    糖基转移酶基因gtfE来自Amycolatopsis orentalis

    公开(公告)号:US06566100B2

    公开(公告)日:2003-05-20

    申请号:US09789261

    申请日:2001-02-20

    IPC分类号: C12P1944

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码所述酶的基因的载体,用于表达所述酶的转化的异源宿主细胞,以及使用编码所述酶的克隆基因产生糖肽化合物的方法。

    Glucosltransferase gene GTFE from amycolatopsis orientalis
    5.
    发明授权
    Glucosltransferase gene GTFE from amycolatopsis orientalis 失效
    来自无性繁殖体的葡萄糖转移酶基因GTFE

    公开(公告)号:US5932464A

    公开(公告)日:1999-08-03

    申请号:US120053

    申请日:1998-07-21

    IPC分类号: C12N1/21 C12N9/10

    CPC分类号: C12N9/1048

    摘要: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode the enzyme, transformed heterologous host cells for expressing the enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode the enzyme.

    摘要翻译: 本发明提供了分离的核酸化合物,其编码无性繁殖体的糖基转移酶。 还提供了携带编码酶的基因的载体,用于表达酶的转化的异源宿主细胞,以及使用编码该酶的克隆基因产生糖肽化合物的方法。

    Glycosyltransferase gene gtfC from Amycolatopsis orientalis
    8.
    发明授权
    Glycosyltransferase gene gtfC from Amycolatopsis orientalis 失效
    来自双孢杆菌的糖基转移酶基因gtfC

    公开(公告)号:US6027928A

    公开(公告)日:2000-02-22

    申请号:US120249

    申请日:1998-07-21

    CPC分类号: C12N9/1048 Y10S435/911

    摘要: The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfC of Amycolatopsis orientalis. Also provided are vectors carrying the gtfC gene, transformed heterologous host cells for expressing the GtfC protein, and methods for producing glycopeptide compounds using the cloned gtfC gene.

    摘要翻译: 本发明提供了编码双歧杆菌的糖基转移酶蛋白GtfC的分离的核酸化合物。 还提供了携带gtfC基因的载体,用于表达GtfC蛋白的转化的异源宿主细胞,以及使用克隆的gtfC基因产生糖肽化合物的方法。

    Method of isolating antibiotic biosynthetic genes
    10.
    发明授权
    Method of isolating antibiotic biosynthetic genes 失效
    分离抗生素生物合成基因的方法

    公开(公告)号:US4935340A

    公开(公告)日:1990-06-19

    申请号:US742349

    申请日:1985-06-07

    CPC分类号: C12N15/76 C12N15/52

    摘要: The present invention is a method for isolating antibiotic biosynthetic genes. To practice the method, an antibiotic resistance-conferring DNA segment is labelled and used as a probe to find, via DNA hydridization, homologous DNA in a genetic library which comprises chromosomal and plasmid DNA of an antibiotic-producing organism. Individual vectors of the genetic library which hybridize to the antibiotic resistance-conferring gene, and which comprise .about.1-45 kb of contiguous DNA from the antibiotic-producing organism, which also comprise an antibiotic biosynthetic gene. The present method is exemplified by using the erythromycin resistance-conferring gene of Streptomyces erythreus to clone the erythromycin biosynthetic pathway from the same organism. The erythromycin biosynthetic pathway isolated with the present method synthesizes erythromycin when introduced into S. lividans TK23.

    摘要翻译: 本发明是分离抗生素生物合成基因的方法。 为了实施该方法,将抗生素抗性赋予DNA片段标记并用作探针,通过DNA杂交在包含产生抗生素的生物体的染色体和质粒DNA的遗传文库中发现同源DNA。 与抗生素耐药基因杂交的基因文库的单个载体,其包含来自产生抗生素的生物体的差异1-45kb的连续DNA,其也包含抗生素生物合成基因。 本发明的方法通过使用红霉素抗性赋予基因,从相同的生物体克隆红霉素生物合成途径。 用本发明方法分离的红霉素生物合成途径将红霉素引入青光眼链霉菌TK23后合成红霉素。