公开(公告)号：US06632662B1

公开(公告)日：2003-10-14

申请号：US09743723

申请日：2001-03-02

申请人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

发明人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

IPC分类号： C12M133

CPC分类号： C12N1/066 ,  C12M47/06

摘要： The invention concerns a device for lysis (1) of micro-organism to release at least one intracellular biological constituent, comprising a container (2) wherein are present a biological sample, in liquid medium, containing the micro-organism to be lyzed, and a material in the form of particles, relatively hard and inert with respect to the sample. The invention also concerns a grinding method. The material in the form of particles comprises at least two types of grinding means into different dimensions: at least large dimension magnetic means (3) automatically controlled by a magnetic field; and at least small dimension means (4) actuated by the large dimension grinding means (3). The invention is useful for separating biological constituents.

摘要翻译： 本发明涉及用于释放至少一种细胞内生物成分的微生物的裂解装置（ 1 ），其包含容器（ 2 ），其中存在生物样品，在含有要溶解的微生物的液体培养基中，以及相对于样品相对较硬且惰性的颗粒形式的材料。 本发明还涉及研磨方法。 颗粒形式的材料包括至少两种不同尺寸的研磨装置：由磁场自动控制的至少大尺寸磁性装置（ 3 ）; 以及由大尺寸研磨装置（ 3 ）致动的至少小尺寸装置（ 4 ）。 本发明可用于分离生物成分

公开(公告)号：US20060223121A1

公开(公告)日：2006-10-05

申请号：US11450360

申请日：2006-06-12

申请人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

发明人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

IPC分类号： G01N33/53 ,  C07K14/74 ,  C07K16/28

CPC分类号： G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

摘要： The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

公开(公告)号：US07081345B1

公开(公告)日：2006-07-25

申请号：US10030937

申请日：2000-07-17

申请人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Hélène Charles ,  Carine Malcus ,  Lyse Santoro ,  Hervé Perron

发明人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Hélène Charles ,  Carine Malcus ,  Lyse Santoro ,  Hervé Perron

IPC分类号： G01N33/53

CPC分类号： G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

摘要： The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

公开(公告)号：US07510843B2

公开(公告)日：2009-03-31

申请号：US11450360

申请日：2006-06-12

申请人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

发明人： Dominique Roecklin ,  Hanno Kolbe ,  Marie-Helene Charles ,  Carine Malcus ,  Lyse Santoro ,  Herve Perron

IPC分类号： G01N33/53 ,  C07K16/18

CPC分类号： G01N33/564 ,  A61K38/1709 ,  C07K14/47 ,  G01N33/6896 ,  G01N2800/52

摘要： The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 10 to SEQ ID No 29, and the peptide sequences or fragments of said sequences belonging to a common family of proteins selected among perlecan, the precursor of the retinol-binding plasmatic protein, of the GM2 activator protein, of calgranulin B and of saponin B.

摘要翻译： 本发明涉及使用包含蛋白质片段的至少一种多肽以获得用于检测，预防或治疗与退行性和/或神经和/或自身免疫疾病相关的病理状况的诊断，预后，预防或治疗组合物，所述蛋白质 选自天然状态的肽序列的蛋白质对应于SEQ ID No 1，SEQ ID No 2，SEQ ID No 3，SEQ ID No 4，SEQ ID No 5，SEQ ID No 6，SEQ ID No 7，SEQ ID No 8，SEQ ID No 9，SEQ ID No 10，SEQ ID No 11，SEQ ID No 12，SEQ ID No 13，SEQ ID No 14，SEQ ID No 15，SEQ ID No 16，SEQ ID No 17，SEQ ID No 18，SEQ ID No 19，SEQ ID No 20，SEQ ID No 21，SEQ ID No 22，SEQ ID No 23，SEQ ID No 24，SEQ ID No 25，SEQ ID No 26，SEQ ID No 27，SEQ ID No 28和SEQ ID No 29，以及肽序列与肽序列SEQ ID No.1至SEQ ID NO：1至SEQ ID NO：1中的任何一个具有至少70％同一性，优选至少80％同一性，有利地至少98％的同一性 ID No 8和SEQ ID No 10至SEQ ID No 29，并且属于共同家族蛋白质的所述序列的肽序列或片段，其选自Perlecan，视黄醇结合性血浆蛋白的前体，GM2激活蛋白， 的钙粒蛋白B和皂角苷B.