公开(公告)号：US06632662B1

公开(公告)日：2003-10-14

申请号：US09743723

申请日：2001-03-02

申请人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

发明人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

IPC分类号： C12M133

CPC分类号： C12N1/066 ,  C12M47/06

摘要： The invention concerns a device for lysis (1) of micro-organism to release at least one intracellular biological constituent, comprising a container (2) wherein are present a biological sample, in liquid medium, containing the micro-organism to be lyzed, and a material in the form of particles, relatively hard and inert with respect to the sample. The invention also concerns a grinding method. The material in the form of particles comprises at least two types of grinding means into different dimensions: at least large dimension magnetic means (3) automatically controlled by a magnetic field; and at least small dimension means (4) actuated by the large dimension grinding means (3). The invention is useful for separating biological constituents.

摘要翻译： 本发明涉及用于释放至少一种细胞内生物成分的微生物的裂解装置（ 1 ），其包含容器（ 2 ），其中存在生物样品，在含有要溶解的微生物的液体培养基中，以及相对于样品相对较硬且惰性的颗粒形式的材料。 本发明还涉及研磨方法。 颗粒形式的材料包括至少两种不同尺寸的研磨装置：由磁场自动控制的至少大尺寸磁性装置（ 3 ）; 以及由大尺寸研磨装置（ 3 ）致动的至少小尺寸装置（ 4 ）。 本发明可用于分离生物成分

公开(公告)号：US06686195B1

公开(公告)日：2004-02-03

申请号：US09937992

申请日：2001-12-13

申请人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

发明人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

IPC分类号： C12M133

CPC分类号： B01J19/10 ,  B06B3/00 ,  C12M45/02

摘要： An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

摘要翻译： 一种装置，包括至少一个超声波发生器（2），其被设计成在至少一个含有待裂解细胞的生物样品（5）中产生可变功率的超声波，所述样品（5）包含在至少一个容器（4或10）中， 超声波发生器（2）与容器（4）直接接触。 还公开了一种使用超声波来溶解包含在容器（4）中的生物样品（5）的方法，其包括将容器（4）放置在与超声焊极（2）直接接触的位置，并且激活超声焊极（2）以用于 足以裂解样品（5）中的细胞，但保留释放用于后续操作的DNA和/或RNA分子，例如 放大。 本发明特别适用于分子生物学学科。

公开(公告)号：US07537730B2

公开(公告)日：2009-05-26

申请号：US10362873

申请日：2001-08-27

申请人： Bruno Colin ,  Cécile Paris ,  Bernard Limon ,  Patrick Broyer ,  Charles Rogers

发明人： Bruno Colin ,  Cécile Paris ,  Bernard Limon ,  Patrick Broyer ,  Charles Rogers

IPC分类号： F16K35/00 ,  B01L11/00 ,  G01N21/00 ,  F17D1/16

CPC分类号： B01L3/502738 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0638 ,  B01L2400/0655 ,  G01N2035/00247 ,  G01N2035/00257 ,  Y10T137/0396

摘要： A reaction card (1) having a body, a front surface (62) and a rear surface (63) defined by an edge (68), at least one inlet (2, 3, 4 and/or 5) and at least one outlet (7and/or 8), connected by a network of channels (64) constituting at least one reaction path for at least one fluid which is directed via valves (11to 36 and/or 61); each valve includes a flexible film (67) which can be deformed to allow fluid passage, the film being fixed on the card's rear surface at a peripheral indentation of an assembly of channels associated with the valve; the card includes channels flush with at least one of its surfaces, the channels being of two different cross-sections, a small cross-section serving as a reaction compartment; each front or rear surface is delimited by at least one film (48, 49, 65, 66 and/or 67).

摘要翻译： 具有主体的反应卡（1），由边缘（68）限定的前表面（62）和后表面（63），至少一个入口（2,3,4和/或5）和至少一个 出口（7和/或8）通过构成用于至少一个通过阀（11至36和/或61）的流体的至少一个反应路径的通道网络（64）连接; 每个阀包括柔性膜（67），其可以变形以允许流体通过，所述膜被固定在卡的后表面上与阀相关联的通道组件的周边凹陷处; 卡包括与其表面中的至少一个平齐的通道，通道具有两个不同的横截面，小的横截面用作反应室; 每个前表面或后表面由至少一个膜（48,49,65,66和/或67）限定。

公开(公告)号：US07159618B2

公开(公告)日：2007-01-09

申请号：US10559454

申请日：2004-06-15

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F15C1/04 ,  F15C1/06

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0036 ,  F16K99/0067 ,  F16K2099/0074 ,  F16K2099/008 ,  Y10T137/2082 ,  Y10T137/2196 ,  Y10T137/2224

摘要： The invention relates to an electrically-opened micro fluid-valve. The inventive valve comprises: at least one planar support (12); at least one afferent microchannel (14); at least one efferent microchannel (16), said microchannels being arranged in the support (12); such that at least one of the ends (141 and 161) thereof touch the inner face (121) of the support close to one another; at least one deposit (18) of a heat-sensitive material which is disposed on the inner face (121) of the support (12); and at least one heating means (20). According to the invention, the aforementioned deposit (18) of heat-sensitive material at least partially seals the space separating the ends (141 and 161) of the microchannels (14 and 16), thereby preventing same from intercommunicating. When the temperature of the above-mentioned heating means (20) is raised, the structure of the heat-sensitive material is altered, thereby at least partially opening the ends of the microchannels an the space therebetween, in order to enable communication between said microchannels and to restore the flow of the fluid.

摘要翻译： 本发明涉及一种电开式微流体阀。 本发明的阀包括：至少一个平面支撑件（12）; 至少一个传入微通道（14）; 至少一个传出微通道（16），所述微通道布置在所述支撑件（12）中; 使得其端部（141和161）中的至少一个接触彼此的支撑件的内表面（121）; 设置在所述支撑件（12）的内表面（121）上的至少一个热敏材料沉积物（18）; 和至少一个加热装置（20）。 根据本发明，上述热敏材料沉积物（18）至少部分地密封分隔微通道（14和16）的端部（141和161）的空间，从而防止其相互通信。 当上述加热装置（20）的温度升高时，热​​敏材料的结构被改变，从而至少部分地将微通道的端部打开它们之间的空间，以便使所述微通道之间的连通 并恢复流体的流动。

公开(公告)号：US07134445B2

公开(公告)日：2006-11-14

申请号：US10531947

申请日：2003-11-18

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F16K7/12 ,  F15B15/19 ,  B81B3/00

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0015 ,  F16K99/0067 ,  F16K2099/008 ,  H01H1/0036 ,  H01H39/00 ,  Y10T137/1647

摘要： The invention concerns the technical field of micro-actuators used for mechanical, chemical, electrical or thermal functions in microsystems, for microelectronic applications such as chips, or biomedical functions such as microfluidics integrating cards. The invention concerns a micro-actuator comprising a so-called main chamber, made in a solid support and containing a so-called main pyrotechnic charge, the main chamber being sealed and delimited by solid support walls and by a deformable membrane, such that the gases emitted by the combustion of the main pyrotechnic charge, enable the volume of the chamber to be increased by deforming the membrane while maintaining intact the solid walls of the main chamber. The invention is characterized in that it comprises means for evacuating the gases from the main chamber.

摘要翻译： 本发明涉及用于微系统中的机械，化学，电或热功能的微致动器的技术领域，用于微电子应用如芯片或生物医学功能，例如集成卡的微流体。 本发明涉及一种微型致动器，其包括所谓的主室，其形成为固体支撑并且包含所谓的主要烟火充电，所述主室由实心支撑壁和可变形膜密封并界定，使得 通过主烟火装置的燃烧发出的气体使得能够通过使膜变形而保持室的体积，同时保持主室的实心壁。 本发明的特征在于其包括用于从主室排出气体的装置。

公开(公告)号：US20060096486A1

公开(公告)日：2006-05-11

申请号：US10552583

申请日：2004-04-09

申请人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

发明人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

IPC分类号： F42C19/08

CPC分类号： F42B3/10 ,  F41A19/65 ,  F42B3/006 ,  F42C19/12

摘要： The invention relates to a device for the electrical initiation of at least one pyrotechnic microcharge (3), this device being characterized in that it comprises a support element having at least one conductive finger (6) connected to a first terminal of a central control unit (8), a second terminal of said central control unit (8) being intended to be electrically connected to an electrically conductive support for the pyrotechnic microcharge (3). The invention also relates to a microactuator (1, 1a, . . . , 1h) and a microsystem (1′) that use such an initiation device.

摘要翻译： 本发明涉及一种用于电启动至少一个烟火微电池（3）的装置，该装置的特征在于其包括具有至少一个导电指状物（6）的支撑元件，该导电指状物（6）连接到中央控制单元 （8），所述中央控制单元（8）的第二端子旨在电连接到用于烟火微电池（3）的导电支撑件。 本发明还涉及使用这种引发装置的微致动器（1,1a，...，1h）和微系统（1'）。

公开(公告)号：US07587977B2

公开(公告)日：2009-09-15

申请号：US10585356

申请日：2005-01-20

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F15B15/19

CPC分类号： F16K99/0001 ,  F15B15/19 ,  F15C5/00 ,  F16K99/0015 ,  F16K99/0067 ,  F16K2099/008 ,  F42B3/04 ,  F42C19/06 ,  F42C19/12 ,  Y10T428/261 ,  Y10T428/31

摘要： The invention relates to a microsystem and a method for fabricating a microsystem. The pyrotechnic microsystem (7, 1′) comprises a substrate having at least two separate electrical initiation zones, each of which provides separate electrical initiation of a pyrotechnic material deposited on the substrate. This microsystem (7, 1′) is characterized in that the same pyrotechnic material deposit (721, 721′, 13) covers both initiation zones, said deposit (721, 721′, 13) produced on the substrate having a thickness sufficiently small for the initiation of the pyrotechnic material in the initiation zone to remain localized and not propagate to the other initiation zone, but sufficient to generate a specific gas quantity.

摘要翻译： 本发明涉及微系统和微系统的制造方法。 烟火微系统（7,1'）包括具有至少两个分开的电引发区的衬底，每个电引发区提供沉积在衬底上的烟火材料的单独的电引发。 该微系统（7,1'）的特征在于，相同的烟火材料沉积物（721,721'，13）覆盖两个起始区域，所述沉积物（721,721'，13）在基板上产生的厚度足够小， 起始区域中的烟火材料的起始保持局部化并且不会传播到另一个起始区，但足以产生特定的气体量。

公开(公告)号：US07270059B2

公开(公告)日：2007-09-18

申请号：US10552583

申请日：2004-04-09

申请人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

发明人： Denis Roller ,  Patrick Broyer ,  Bruno Colin

IPC分类号： F42B3/10

CPC分类号： F42B3/10 ,  F41A19/65 ,  F42B3/006 ,  F42C19/12

摘要： The invention relates to a device for the electrical initiation of at least one pyrotechnic microcharge (3), this device being characterized in that it comprises a support element having at least one conductive finger (6) connected to a first terminal of a central control unit (8), a second terminal of said central control unit (8) being intended to be electrically connected to an electrically conductive support for the pyrotechnic microcharge (3). The invention also relates to a microactuator (1, 1a, . . . , 1h) and a microsystem (1′) that use such an initiation device.

摘要翻译： 本发明涉及一种用于电启动至少一个烟火微电池（3）的装置，该装置的特征在于其包括具有至少一个导电指状物（6）的支撑元件，该导电指状物（6）连接到中央控制单元 （8），所述中央控制单元（8）的第二端子旨在电连接到用于烟火微电池（3）的导电支撑件。 本发明还涉及使用这种引发装置的微致动器（1,1a，...，1h）和微系统（1'）。

公开(公告)号：US20060180223A1

公开(公告)日：2006-08-17

申请号：US10559454

申请日：2004-06-15

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F15C1/04

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0036 ,  F16K99/0067 ,  F16K2099/0074 ,  F16K2099/008 ,  Y10T137/2082 ,  Y10T137/2196 ,  Y10T137/2224

摘要： The present invention relates to an electrically openable microfluidic valve, which comprises: at least one plane support (12); at least one afferent microchannel (14); at least one efferent microchannel (16); said microchannels being provided in the support, so that at least one of their ends (141) and (161) opens onto the internal face (121) of said support (12), near one another; at least one deposit (18) of a heat-sensitive material laid out on the internal face (121) of the support (12); and at least one heating means (20); so that the deposit (18) of heat-sensitive material at least partly closes off the space separating the ends (141) and (161) of the microchannels (14) and (16), thus preventing the latter from communicating with one another; the increase in temperature by the heating means (20) resulting in a modification of the structure of the heat-sensitive material, thus at least partially clearing the ends of the microchannels and the space between the latter, so as to allow communication between said microchannels and to restore flow of the fluid.

摘要翻译： 本发明涉及一种可电开式微流体阀，其包括：至少一个平面支撑件（12）; 至少一个传入微通道（14）; 至少一个传出微通道（16）; 所述微通道设置在所述支撑件中，使得其端部（141）和（161）中的至少一个在所述支撑件（12）的内表面（121）上彼此靠近地开口; 布置在所述支撑件（12）的内表面（121）上的至少一个热敏材料沉积物（18）; 和至少一个加热装置（20）; 使得热敏材料的沉积物（18）至少部分地封闭分开微通道（14）和（16）的端部（141）和（161）的空间，从而防止热敏材料彼此连通; 加热装置（20）的温度升高导致热敏材料的结构的改变，从而至少部分地清除微通道的端部和后者之间的空间，以便允许所述微通道之间的连通 并恢复流体的流动。

公开(公告)号：US20080041259A1

公开(公告)日：2008-02-21

申请号：US10585356

申请日：2005-01-20

申请人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

发明人： Patrick Broyer ,  Bruno Colin ,  Denis Roller

IPC分类号： F42C19/12 ,  B05D1/02 ,  B05D1/18 ,  B05D1/36 ,  B32B27/00

CPC分类号： F16K99/0001 ,  F15B15/19 ,  F15C5/00 ,  F16K99/0015 ,  F16K99/0067 ,  F16K2099/008 ,  F42B3/04 ,  F42C19/06 ,  F42C19/12 ,  Y10T428/261 ,  Y10T428/31

摘要： The invention relates to a microsystem and a method for fabricating a microsystem. The pyrotechnic microsystem (7, 1′) comprises a substrate having at least two separate electrical initiation zones, each of which provides separate electrical initiation of a pyrotechnic material deposited on the substrate. This microsystem (7, 1′) is characterized in that the same pyrotechnic material deposit (721, 721′, 13) covers both initiation zones, said deposit (721, 721′, 13) produced on the substrate having a thickness sufficiently small for the initiation of the pyrotechnic material in the initiation zone to remain localized and not propagate to the other initiation zone, but sufficient to generate a specific gas quantity.

摘要翻译： 本发明涉及微系统和微系统的制造方法。 烟火微系统（7,1'）包括具有至少两个分开的电引发区的衬底，每个电引发区提供沉积在衬底上的烟火材料的单独的电引发。 该微系统（7,1'）的特征在于，相同的烟火材料沉积物（721,721'，13）覆盖两个起始区域，所述沉积物（721,721'，13）在基板上产生的厚度足够小， 起始区域中的烟火材料的起始保持局部化并且不会传播到另一个起始区，但足以产生特定的气体量。