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    Methods for in situ generation of nucleic acid arrays
    4.
    发明申请
    Methods for in situ generation of nucleic acid arrays 审中-公开
    原位产生核酸阵列的方法

    公开(公告)号：US20050214778A1

    公开(公告)日：2005-09-29

    申请号：US10813337

    申请日：2004-03-29

    申请人： Bill Peck ,  Eric Leproust

    发明人： Bill Peck ,  Eric Leproust

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00722

    摘要： Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step. A feature of the subject methods is that the functional group generation step is performed by sequentially flowing a plurality of different fluids across the surface of a substrate in a manner such that the surface is not contacted with a with a triple phase interface line. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.

    摘要翻译： 提供了使用原位核酸合成方案生产核酸阵列的方法，其中原位核酸合成方案包括多个循环，每个循环包括：（I）单体附着步骤; 和（II）官能团生成步骤。 本发明方法的特征在于，功能组生成步骤是通过使表面不与三相界面线接触的方式使多个不同的流体顺序地流过衬底的表面而进行的。 还提供了使用主题方法产生的阵列，以及使用包括其的阵列和试剂盒的方法。

    Method, system, and computer program product to assess properties of a chemical array
    7.
    发明申请
    Method, system, and computer program product to assess properties of a chemical array 审中-公开
    方法，系统和计算机程序产品来评估化学数组的性质

    公开(公告)号：US20060078896A1

    公开(公告)日：2006-04-13

    申请号：US10964063

    申请日：2004-10-12

    申请人： Bill Peck ,  Eric Leproust

    发明人： Bill Peck ,  Eric Leproust

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00 ,  C40B40/08

    CPC分类号： G16B25/00 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00702 ,  C40B40/08

    摘要： A method, a system, and a computer-readable medium storing a program for determining array quality during in-situ manufacturing of an array includes determining an average feature coverage fraction of the array, determining a background area fraction of the array, measuring a contact angle of the background, and determining the array quality based upon the measured, feature contact angle.

    摘要翻译： 存储用于在阵列的原位制造期间确定阵列质量的程序的方法，系统和计算机可读介质包括确定阵列的平均特征覆盖率分数，确定阵列的背景区域分数，测量接触 背景的角度，以及基于所测量的特征接触角确定阵列质量。

    Methods for evaluating tissue pair combinations for use in nucleic acid array technology
    8.
    发明申请
    Methods for evaluating tissue pair combinations for use in nucleic acid array technology 审中-公开
    评估用于核酸阵列技术的组织对组合的方法

    公开(公告)号：US20050112584A1

    公开(公告)日：2005-05-26

    申请号：US10718342

    申请日：2003-11-20

    申请人： Francisco Cifuentes ,  Meha Kapadia ,  Eric Leproust ,  Stephanie Fulmer-Smentek ,  Bill Peck

    发明人： Francisco Cifuentes ,  Meha Kapadia ,  Eric Leproust ,  Stephanie Fulmer-Smentek ,  Bill Peck

    IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

    CPC分类号： G16B25/00

    摘要： Methods are disclosed for selecting a combination of nucleic acid sample pairs for evaluating the ability of an oligonucleotide probe to measure differential expression of genes. Differential gene expression experiments are conducted using (i) nucleic acid sample pairs and (ii) nucleic acid probes immobilized on a substrate, the probes representing a set of genes. The number of genes in the set is a portion of an expected number of genes in a sample. A nucleic acid sample pair combination is selected based on the members of the combination having a maximized number of genes from the set of genes that exhibit differential expression and a minimized number of the genes that do not exhibit differential expression.

    摘要翻译： 公开了用于选择核酸样品对的组合以评估寡核苷酸探针以测量基因差异表达的能力的方法。 使用（i）核酸样品对和（ii）固定在底物上的核酸探针进行差异基因表达实验，探针代表一组基因。 该组中的基因数目是样品中预期基因数量的一部分。 基于组合的成员选择核酸样品对组合，其具有来自显示差异表达的基因组的最大数量的基因和不显示差异表达的基因的最小数目。