公开(公告)号：US06221612B1

公开(公告)日：2001-04-24

申请号：US09122477

申请日：1998-07-23

申请人： Tom Knapp ,  Gregor Zlokarnik ,  Paul Negulescu ,  Roger Y. Tsien ,  Tim Rink

发明人： Tom Knapp ,  Gregor Zlokarnik ,  Paul Negulescu ,  Roger Y. Tsien ,  Tim Rink

IPC分类号： C12Q170

CPC分类号： G01N33/582 ,  C12Q1/682 ,  G01N33/5005 ,  G01N33/542 ,  Y10S436/80 ,  Y10S436/809 ,  Y10T436/13 ,  C12Q2563/107 ,  C12Q2527/125

摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.

摘要翻译： 本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。 本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。 本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

公开(公告)号：US07067324B2

公开(公告)日：2006-06-27

申请号：US09759629

申请日：2001-01-12

申请人： Tom Knapp ,  Gregor Zlokarnik ,  Paul Negulescu ,  Roger Y. Tsien ,  Tim Rink

发明人： Tom Knapp ,  Gregor Zlokarnik ,  Paul Negulescu ,  Roger Y. Tsien ,  Tim Rink

IPC分类号： C12Q1/70 ,  G01N21/76

CPC分类号： G01N33/582 ,  C12Q1/682 ,  G01N33/5005 ,  G01N33/542 ,  Y10S436/80 ,  Y10S436/809 ,  Y10T436/13 ,  C12Q2563/107 ,  C12Q2527/125

摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.

公开(公告)号：US06214563B1

公开(公告)日：2001-04-10

申请号：US09120516

申请日：1998-07-21

申请人： Paul Negulescu ,  Gregor Zlokarnik ,  Tom Knapp ,  Roger Y. Tsien ,  Tim Rink

发明人： Paul Negulescu ,  Gregor Zlokarnik ,  Tom Knapp ,  Roger Y. Tsien ,  Tim Rink

IPC分类号： C12Q170

CPC分类号： G01N33/542 ,  C12Q1/34 ,  C12Q1/682 ,  G01N33/5005 ,  G01N2415/00 ,  Y10S436/80 ,  Y10S436/809 ,  C12Q2563/107 ,  C12Q2527/125

摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions and kits for practicing these methods.

摘要翻译： 本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。 本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供使用至少一种光子还原剂测定样品中结合和游离分析物的方法。 本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的方法。 本发明还提供了用于实施这些方法的组合物和试剂盒。

公开(公告)号：US06200762B1

公开(公告)日：2001-03-13

申请号：US09118497

申请日：1998-07-17

申请人： Gregor Zlokarnik ,  Paul Negulescu ,  Tom Knapp ,  Roger Y. Tsien ,  Tim Rink

发明人： Gregor Zlokarnik ,  Paul Negulescu ,  Tom Knapp ,  Roger Y. Tsien ,  Tim Rink

IPC分类号： C12Q170

CPC分类号： G01N33/542 ,  C12Q1/682 ,  G01N33/5005 ,  Y10S436/80 ,  Y10S436/809 ,  C12Q2563/107 ,  C12Q2527/125

摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions and kits for practicing these methods.

摘要翻译： 本发明提供一种使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。 本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（例如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。 本发明还提供使用至少一种光子还原剂测定样品中结合和游离分析物的方法。 本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的方法。 本发明还提供了用于实施这些方法的组合物和试剂盒。