公开(公告)号：US10428364B2

公开(公告)日：2019-10-01

申请号：US16380678

申请日：2019-04-10

Applicant: PepsiCo, Inc.

Inventor： Junhua Tao ,  Guoqing Li ,  Xiaoliang Liang ,  Andrew Tao

IPC: C12P19/58 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N9/10 ,  C12P19/56 ,  A23L27/30 ,  C12P19/18

Abstract: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.

公开(公告)号：US10301662B2

公开(公告)日：2019-05-28

申请号：US14911876

申请日：2013-09-29

Applicant: PepsiCo, Inc.

Inventor： Junhua Tao ,  Guoqing Li ,  Xiaoliang Liang ,  Andrew Tao

IPC: C12P19/56 ,  C12N9/10 ,  C07H21/04 ,  C12N15/00 ,  A23L27/30 ,  C12P19/18

Abstract: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.