公开(公告)号：US11976312B2

公开(公告)日：2024-05-07

申请号：US17657307

申请日：2022-03-30

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/56 ,  C12N9/10

CPC classification number: C12P19/56 ,  C12N9/10 ,  C12Y204/01

Abstract: Provided is a method for preparing Rebaudioside C using an enzymatic method, comprising using rubusoside or dulcoside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside C.

公开(公告)号：US11352653B2

公开(公告)日：2022-06-07

申请号：US16343335

申请日：2016-10-21

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/56 ,  C12N9/10 ,  C12P7/40

Abstract: Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US11976313B2

公开(公告)日：2024-05-07

申请号：US17663631

申请日：2022-05-16

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/18 ,  C12N9/10 ,  C12P19/56 ,  C12P7/40

CPC classification number: C12P19/56 ,  C12N9/1048 ,  C12N9/10 ,  C12P7/40 ,  C12Y204/01017

Abstract: Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.

公开(公告)号：US11359222B2

公开(公告)日：2022-06-14

申请号：US16343340

申请日：2016-10-21

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/56 ,  C12N9/10

Abstract: Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-con-taining recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US10301662B2

公开(公告)日：2019-05-28

申请号：US14911876

申请日：2013-09-29

Applicant: PepsiCo, Inc.

Inventor： Junhua Tao ,  Guoqing Li ,  Xiaoliang Liang ,  Andrew Tao

IPC: C12P19/56 ,  C12N9/10 ,  C07H21/04 ,  C12N15/00 ,  A23L27/30 ,  C12P19/18

Abstract: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.

公开(公告)号：US11952604B2

公开(公告)日：2024-04-09

申请号：US17805626

申请日：2022-06-06

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/56 ,  C12N9/10

CPC classification number: C12P19/56 ,  C12N9/1051 ,  C12Y204/01017

Abstract: Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.

公开(公告)号：US11312985B2

公开(公告)日：2022-04-26

申请号：US16343339

申请日：2016-10-21

Applicant: PepsiCo, Inc.

Inventor： Alex Tao ,  Guoqing Li ,  Wenxia Wang ,  Leilei Zheng ,  Chunlei Zhu ,  Xiaoliang Liang ,  Kuikiu Chan

IPC: C12P19/18 ,  C12P19/56 ,  C12N9/10

Abstract: Provided is a method for preparing Rebaudioside C using an enzymatic method, comprising using rubusoside or dulcoside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside C.

公开(公告)号：US10428364B2

公开(公告)日：2019-10-01

申请号：US16380678

申请日：2019-04-10

Applicant: PepsiCo, Inc.

Inventor： Junhua Tao ,  Guoqing Li ,  Xiaoliang Liang ,  Andrew Tao

IPC: C12P19/58 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N9/10 ,  C12P19/56 ,  A23L27/30 ,  C12P19/18

Abstract: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.