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公开(公告)号：US08871212B2
公开(公告)日：2014-10-28
申请号：US12339841
申请日：2008-12-19
申请人： Peter Birk Rasmussen , Martin Roland Jensen , Klaus Gregorius Nielsen , Peter Koefoed , Florence Dal Degan
发明人： Peter Birk Rasmussen , Martin Roland Jensen , Klaus Gregorius Nielsen , Peter Koefoed , Florence Dal Degan
IPC分类号： A61K39/00 , A61K39/385 , A61K39/08 , A01K67/027 , C07K14/47
CPC分类号： A61K39/0007 , A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/0312 , A61K39/385 , A61K45/06 , A61K2039/53 , A61K2039/6037 , A61K2039/6068 , A61K2039/6087 , A61K2039/6093 , A61K2039/64 , C07K14/4711 , Y02A50/412
摘要： Disclosed are novel methods and compositions for combating diseases characterized by deposition of amyloid. The methods generally rely on immunization against amyloid precursor protein (APP) or beta amyloid (Aβ). Immunization is preferably effected by administration of analogs of autologous APP or Aβ, said analogs being capable of inducing antibody production against the autologous amyloidogenic polypeptides. Especially preferred as an immunogen is autologous Aβ which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes. Such methods and means include methods for the preparation of analogs and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.
摘要翻译： 公开了用于防治以沉淀淀粉样蛋白为特征的疾病的新颖方法和组合物。 所述方法通常依赖于针对淀粉样前体蛋白（APP）或β淀粉样蛋白（A＆bgr）的免疫。 免疫优选通过施用自体APP或A＆bgr的类似物来实现;所述类似物能够诱导针对自体淀粉样蛋白形成多肽的抗体产生。 特别优选作为免疫原是自体A＆bgr; 其通过引入一种或几种外来的免疫显性和混杂的T细胞表位而被修饰。 这些方法和手段包括制备类似物和药物制剂以及核酸片段，载体，转化细胞，多肽和药物制剂的方法。
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公开(公告)号：US20100047262A1
公开(公告)日：2010-02-25
申请号：US12339841
申请日：2008-12-19
申请人： Peter Birk Rasmussen , Martin Roland Jensen , Klaus Gregorius Nielsen , Peter Koefoed , Florence Dal Degan
发明人： Peter Birk Rasmussen , Martin Roland Jensen , Klaus Gregorius Nielsen , Peter Koefoed , Florence Dal Degan
CPC分类号： A61K39/0007 , A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/0312 , A61K39/385 , A61K45/06 , A61K2039/53 , A61K2039/6037 , A61K2039/6068 , A61K2039/6087 , A61K2039/6093 , A61K2039/64 , C07K14/4711 , Y02A50/412
摘要： Disclosed are novel methods and compositions for combating diseases characterized by deposition of amyloid. The methods generally rely on immunization against amyloid precursor protein (APP) or beta amyloid (Aβ). Immunization is preferably effected by administration of analogues of autologous APP or Aβ, said analogues being capable of inducing antibody production against the autologous amyloidogenic polypeptides. Especially preferred as an immunogen is autologous Aβ which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes. Such methods and means include methods for the preparation of analogues and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.
摘要翻译： 公开了用于防治以沉淀淀粉样蛋白为特征的疾病的新颖方法和组合物。 所述方法通常依赖于针对淀粉样前体蛋白（APP）或β淀粉样蛋白（A＆bgr）的免疫。 免疫优选通过施用自体APP或A＆bgr的类似物来实现;所述类似物能够诱导针对自体淀粉样变性多肽的抗体产生。 特别优选作为免疫原是自体A＆bgr; 其通过引入一种或几种外来的免疫显性和混杂的T细胞表位而被修饰。 这些方法和手段包括制备类似物和药物制剂以及核酸片段，载体，转化细胞，多肽和药物制剂的方法。
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公开(公告)号：US07135181B2
公开(公告)日：2006-11-14
申请号：US09785215
申请日：2001-02-20
CPC分类号： A61K39/385 , A61K38/00 , A61K38/1709 , A61K39/0007 , A61K47/646 , A61K2039/53 , A61K2039/6037 , A61K2039/6087 , A61K2039/64 , C07K14/4711 , C07K2319/00 , G01N33/6896 , G01N2333/4709 , G01N2500/04
摘要： A method for in vivo down-regulation of amyloid protein in an animal, including a human being, the method comprising effecting presentation to the animal's immune system of an immunogenically effective amount of at least one amyloidogenic polypeptide or subsequence thereof which has been formulated so that immunization of the animal with the amyloidgenic polypeptide or subsequence thereof induces production of antibodies against the amyloidogenic polypeptide, and/or at least one analogue of the amyloidogenic polypeptide wherein is introduced at least one modification in the amino acid sequence of the amyloidogenic polypeptide which has as a result the immunization of the animal with the analogue induces production of antibodies against the amyloidogenic polypeptide.
摘要翻译： 一种用于动物体内下调淀粉样蛋白的方法，包括人，所述方法包括向动物的免疫系统呈递免疫有效量的至少一种淀粉样变性多肽或其子序列，其被配制成使得 用淀粉样蛋白生成多肽或其子序列免疫动物诱导针对淀粉样变性多肽和/或淀粉样变性多肽的至少一种类似物的抗体的产生，其中引入淀粉样变性多肽的氨基酸序列中的至少一个修饰，其具有如 结果，用该类似物的动物的免疫诱导产生针对淀粉样变性多肽的抗体。
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公开(公告)号：US07005498B1
公开(公告)日：2006-02-28
申请号：US09806703
申请日：1999-10-05
申请人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
发明人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
IPC分类号： C07K9/00
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。
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公开(公告)号：US07807441B2
公开(公告)日：2010-10-05
申请号：US11202516
申请日：2005-08-11
申请人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Haaning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
发明人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Haaning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
IPC分类号： C12N1/00 , C12N1/10 , C12N1/14 , C12N1/16 , C12N1/20 , C12N5/10 , C12N5/14 , C12N5/16 , C12N15/00 , A61K38/00 , C07K14/00
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。
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公开(公告)号：US20090092579A1
公开(公告)日：2009-04-09
申请号：US12108478
申请日：2008-04-23
IPC分类号： A61K38/20 , A61K39/00 , C07K14/00 , C12N15/11 , C12N15/00 , C12N1/21 , A61K38/21 , C07K16/18 , C12N1/19 , C12N1/10 , C12N5/06 , A61K38/16 , C12N15/87
CPC分类号： A61K39/385 , A61K38/00 , A61K38/1709 , A61K39/0007 , A61K47/646 , A61K2039/53 , A61K2039/6037 , A61K2039/6087 , A61K2039/64 , C07K14/4711 , C07K2319/00 , G01N33/6896 , G01N2333/4709 , G01N2500/04
摘要： Disclosed are novel methods for combating diseases characterized by deposition of amyloid. The methods generally rely on immunization against amyloidogenic proteins (proteins contributing to formation of amyloid) such as beta amyloid (Aβ). Immunization is preferably effected by administration of analogues of autologous amyloidogenic polypeptides, said analogues being capable of inducing antibody production against the autologous amyloidogenic polypeptides. Especially preferred as an immunogen is autologous Aβ which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes while substantially preserving the majority of Aβ's B-cell epitopes. Also disclosed are nucleic acid vaccination against amyloidogenic polypeptides and vaccination using live vaccines as well as methods and means useful for the vaccination. Such methods and means include methods for identification of useful immunogenic analogues of the amyloidogenic proteins, methods for the preparation of analogues and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.
摘要翻译： 公开了用于对抗以沉淀淀粉样蛋白为特征的疾病的新方法。 所述方法通常依赖于抗淀粉样变蛋白（有助于形成淀粉样蛋白的蛋白质）如β淀粉样蛋白（Abeta）的免疫。 免疫优选通过施用自体淀粉样蛋白多肽的类似物来实现，所述类似物能够诱导针对自体淀粉样蛋白多肽的抗体产生。 作为免疫原特别优选的是通过引入一个或几个外来的免疫显性和混杂的T细胞表位而修饰的自体Abeta，同时基本上保留了大部分的Abeta的B细胞表位。 还公开了针对淀粉样变性多肽的核酸接种和使用活疫苗的疫苗接种以及可用于疫苗接种的方法和装置。 这样的方法和手段包括用于鉴定淀粉样蛋白形成蛋白质的有用的免疫原性类似物的方法，用于制备类似物和药物制剂的方法，以及核酸片段，载体，转化细胞，多肽和药物制剂。
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公开(公告)号：US20090311281A1
公开(公告)日：2009-12-17
申请号：US12360962
申请日：2009-01-28
IPC分类号： A61K39/00
CPC分类号： G01N33/6896 , A61K38/00 , A61K38/1709 , A61K38/19 , A61K39/00 , A61K39/0007 , A61K39/385 , A61K47/646 , A61K2039/53 , A61K2039/6037 , A61K2039/6087 , A61K2039/64 , C07K14/4711 , C07K2319/00 , G01N2333/4709 , G01N2500/04 , Y02A50/412 , A61K2300/00
摘要： Disclosed are novel methods for combating diseases characterized by deposition of amyloid. The methods generally rely on immunization against amyloidogenic proteins (proteins contributing to formation of amyloid) such as beta amyloid (Aβ). Immunization is preferably effected by administration of analogues of autologous amyloidogenic polypeptides, said analogues being capable of inducing antibody production against the autologous amyloidogenic polypeptides. Especially preferred as an immunogen is autologous Aβ which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes while substantially preserving the majority of Aβ's B-cell epitopes. Also disclosed are nucleic acid vaccination against amyloidogenic polypeptides and vaccination using live vaccines as well as methods and means useful for the vaccination. Such methods and means include methods for identification of useful immunogenic analogues of the amyloidogenic proteins, methods for the preparation of analogues and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.
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公开(公告)号：US07097837B2
公开(公告)日：2006-08-29
申请号：US10080101
申请日：2002-02-19
IPC分类号： A61K39/00 , A61K39/385 , A61K38/00 , C07K4/00 , C07K17/00
CPC分类号： A61K39/0007 , A61K9/19 , A61K39/385 , A61K47/61 , A61K2039/53 , A61K2039/6037 , A61K2039/6068 , A61K2039/6087 , A61K2039/6093 , A61K2039/64 , C07K14/4711 , C07K2319/00
摘要： The present invention provides for novel immungens that are comprised of an activated polyhydroxypolymer backbone to which is attached 2 separate antigenic determinants. The 1st antigenic determinant includes a B-cell or CTL epitope and the 2nd antigenic determinant includes a T-helper epitope. In preferred embodiments, the antigenic determinants are derived from different molecules and species. Exemplary immunogens of the invention are constituted of a linear tresyl-activated dextran backbone to which is coupled B-cell or CTL epitopes of an antigen and to which is also coupled universal T-helper epitopes. Also disclosed are immunogenic compositions comprising the immunogens, methods of immunization and a method for identification of suitable immunogens of the invention.
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公开(公告)号：US07763452B2
公开(公告)日：2010-07-27
申请号：US10560961
申请日：2004-06-24
CPC分类号： C07K14/71
摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein for which the purification process has been especially adapted, as well as means for the preparation of the variant.
摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），c）尺寸排阻层析（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。
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公开(公告)号：US08022039B2
公开(公告)日：2011-09-20
申请号：US12824420
申请日：2010-06-28
IPC分类号： A61K38/16 , A61K38/17 , C07K14/00 , C07K14/435 , C07K14/705
CPC分类号： C07K14/71
摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), C) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.
摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），C）尺寸排阻色谱法（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。
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