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公开(公告)号：US07807435B2
公开(公告)日：2010-10-05
申请号：US11202349
申请日：2005-08-11
IPC分类号： C12N9/99
CPC分类号： C07K14/8125
摘要： The present invention relates to a method for the purification of alpha-1 proteinase inhibitor (a1PI) from protein fractions. More specifically, the invention relates to an improved method for the purification of alpha-1 proteinase inhibitor (a1PI), wherein the yield of a1PI can be increased by thawing the starting material and incubating it for several hours before subjecting it to a washing step.
摘要翻译： 本发明涉及从蛋白质级分纯化α-1蛋白酶抑制剂（a1PI）的方法。 更具体地说，本发明涉及一种纯化α-1蛋白酶抑制剂（a1PI）的改进方法，其中可以通过解冻起始物质并将其孵育数小时，然后使其进行洗涤步骤，从而增加a1PI的产率。
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公开(公告)号：US20070037270A1
公开(公告)日：2007-02-15
申请号：US11202349
申请日：2005-08-11
IPC分类号： C12N9/99
CPC分类号： C07K14/8125
摘要： The present invention relates to a method for the purification of alpha-1 proteinase inhibitor (a1PI) from protein fractions. More specifically, the invention relates to an improved method for the purification of alpha-1 proteinase inhibitor (a1PI), wherein the yield of a1PI can be increased by thawing the starting material and incubating it for several hours before subjecting it to a washing step.
摘要翻译： 本发明涉及从蛋白质级分纯化α-1蛋白酶抑制剂（a1PI）的方法。 更具体地说，本发明涉及一种纯化α-1蛋白酶抑制剂（a1PI）的改进方法，其中可以通过解冻起始物质并将其孵育数小时，然后使其进行洗涤步骤，从而增加a1PI的产率。
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公开(公告)号：US20090304669A1
公开(公告)日：2009-12-10
申请号：US12124390
申请日：2008-05-21
CPC分类号： C12N9/6454 , B01D15/363 , C12Y304/21075
摘要： Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC™ resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC™ chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
摘要翻译： 重组截短的人弗林蛋白酶在CHO细胞中表达，并通过超滤和渗滤浓缩约50倍。 浓缩物通过Capto-MMC TM上的柱色谱纯化，得到30-50倍纯化因子，产率至少为60％。 在Capto-MMC TM色谱上获得的至少20％的纯制剂已经具有允许亲VWF的柱上成熟的纯化程度。 然后进行额外的精氨酸琼脂糖层析纯化。 用于纯化截短的人弗林蛋白酶的这种两柱方法产生几乎纯的弗林蛋白酶制剂，其具有约290,000U弗林蛋白/ mg蛋白质的比活性，约50％的产率。
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公开(公告)号：US08343748B2
公开(公告)日：2013-01-01
申请号：US12124390
申请日：2008-05-21
CPC分类号： C12N9/6454 , B01D15/363 , C12Y304/21075
摘要： Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
摘要翻译： 重组截短的人弗林蛋白酶在CHO细胞中表达，并通过超滤和渗滤浓缩约50倍。 浓缩物通过CAPTO MMC TM（混合阳离子交换/疏水相互作用凝胶）上的柱色谱纯化，得到30-50倍纯化因子，产率至少为60％。 在CAPTO MMC TM（混合阳离子交换/疏水相互作用凝胶）色谱之后获得的至少20％的纯制剂已经具有允许亲VWF的柱上成熟的纯化度。 然后进行额外的精氨酸琼脂糖层析纯化。 用于纯化截短的人弗林蛋白酶的这种两柱方法产生几乎纯的弗林蛋白酶制剂，其具有约290,000U弗林蛋白/ mg蛋白质的比活性，约50％的产率。
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5.发明申请METHODS FOR THE INACTIVATION OF MICROORGANISMS IN BIOLOGICAL FLUIDS, FLOW THROUGH REACTORS AND METHODS OF CONTROLLING THE LIGHT SUM DOSE TO EFFECTIVELY INACTIVATE MICROORGANISMS IN BATCH REACTORS 有权生物流体中微生物灭活的方法，通过反应器流动的方法和控制轻剂量以有效地使分批反应器中的微生物失活的方法公开(公告)号：US20110206554A1
公开(公告)日：2011-08-25
申请号：US13100570
申请日：2011-05-04
申请人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
发明人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC分类号： A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
摘要： The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
摘要翻译： 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭活间歇反应器中存在于生物流体中的微生物。
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公开(公告)号：US20060045796A1
公开(公告)日：2006-03-02
申请号：US10924579
申请日：2004-08-24
申请人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel Boggs
发明人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel Boggs
CPC分类号： A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
摘要： The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
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7.发明授权公开(公告)号：US06777390B1
公开(公告)日：2004-08-17
申请号：US09719945
申请日：2001-02-20
IPC分类号： A61K3800
CPC分类号： C12N9/6437 , A61K38/00 , C12Y304/21021
摘要： Stable pharmaceutical preparations containing blood coagulation Factor VII is disclosed. The pharmaceutical preparations containing blood coagulation Factor VII are free of coagulation inhibitors and are stable over a wide range of environmental conditions. Also provided are blood coagulation Factor VII preparations having a minimum activity of 50 Units/mg of protein that contain less than 5% activated blood coagulation Factor VII (Factor VIIa). The blood coagulation Factor VII containing preparations may also contain other blood coagulation factors and are free from detectable transmissible human pathogens.
摘要翻译： 公开了含有凝血因子VII的稳定的药物制剂。 含有凝血因子VII的药物制剂不含凝血抑制剂，并且在宽范围的环境条件下是稳定的。 还提供具有最小活性为50单位/ mg蛋白质的含有少于5％的活化凝血因子VII（因子VIIa）的凝血因子Ⅶ制剂。 含有凝血因子Ⅶ的制剂还可含有其他凝血因子，并且不含可检测的可传播的人类病原体。
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8.发明授权Methods for the inactivation of microorganisms in biological fluids, flow through reactors and methods of controlling the light sum dose to effectively inactivate microorganisms in batch reactors 有权生物流体中微生物灭活的方法，流经反应器的方法和控制光总剂量以有效灭活间歇反应器中的微生物的方法公开(公告)号：US08377375B2
公开(公告)日：2013-02-19
申请号：US13100570
申请日：2011-05-04
申请人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
发明人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC分类号： A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
摘要： The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
摘要翻译： 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭活间歇反应器中存在于生物流体中的微生物。
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9.发明授权Methods for the inactivation of microorganisms in biological fluids, flow through reactors and methods of controlling the light sum dose to effectively inactivate microorganisms in batch reactors 有权生物流体中微生物灭活的方法，流经反应器的方法和控制光总剂量以有效灭活间歇反应器中的微生物的方法公开(公告)号：US07993580B2
公开(公告)日：2011-08-09
申请号：US10924579
申请日：2004-08-24
申请人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
发明人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC分类号： A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
摘要： The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
摘要翻译： 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供了一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭菌间歇反应器中存在于生物流体中的微生物。
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10.发明授权Method for the validatable inactivation of pathogens in a biological fluid by irradiation 有权通过照射可以有效灭活生物体液中病原体的方法公开(公告)号：US08986607B2
公开(公告)日：2015-03-24
申请号：US10547172
申请日：2004-02-27
申请人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
发明人： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
IPC分类号： A61L2/00 , C12N13/00 , G01J1/08 , A61L2/10 , A61L2/28 , G01J1/42 , G01J1/48 , A61M1/36 , C02F1/32
CPC分类号： G01J1/08 , A61L2/0011 , A61L2/0017 , A61L2/0052 , A61L2/10 , A61L2/28 , A61L2202/22 , A61M1/3681 , A61M1/3683 , A61M2205/053 , C02F1/32 , C02F2201/326 , G01J1/42 , G01J1/48
摘要： A validatable method for determining a photochemically effective dose for inactivating pathogens in a fluid sample is described herein. In particular, the instant invention covers methods for determining a photochemically effective dose sufficient to inactivate pathogens in a biological sample while leaving biologically active substances of interest unaffected. A batch irradiation reactor effective for inactivating pathogens in biological samples is also described.
摘要翻译： 本文描述了用于确定用于灭活流体样品中病原体的光化学有效剂量的可验证方法。 特别地，本发明包括确定足以灭活生物样品中的病原体的光化学有效剂量同时使感兴趣的生物活性物质不受影响的方法。 还描述了有效用于灭活生物样品中的病原体的批量照射反应器。
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