公开(公告)号：US20230257317A1

公开(公告)日：2023-08-17

申请号：US17922712

申请日：2021-04-29

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Neal Shah ,  Bilge Ozaydin Eskiyenenturk ,  Sarah Bloch ,  Alvin Tamsir

IPC分类号： C05F11/08 ,  C12N1/20 ,  C12N15/52 ,  C12N15/74

CPC分类号： C05F11/08 ,  C12N1/205 ,  C12N15/52 ,  C12N15/74 ,  C12R2001/22

摘要： Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in a gene regulating nitrogen fixation or assimilation, wherein the modification in the gene regulating nitrogen fixation or assimilation results in one or more of: constitutive expression of a nifA gene in nitrogen limiting and non-nitrogen limiting conditions, activity of nifA in non-nitrogen limiting conditions, decreased uridylyl-transferase activity of GlnD, decreased adenylyl-removing activity of GlnE, and increased ammonium excretion.

公开(公告)号：US20220017911A1

公开(公告)日：2022-01-20

申请号：US17312172

申请日：2019-12-20

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Neal Shah ,  Shayin Gottlieb ,  Richard Broglie ,  Emily Tung ,  Rosemary Clark

IPC分类号： C12N15/74 ,  C12N1/20 ,  C12Q1/04

摘要： Methods of predicting in planta phenotypes of microbial strains are provided. The methods can include culturing microbial strains in a plant exudate medium and assaying in vitro phenotypes of the microbial strains. The methods may also include using the in vitro phenotypes to predict in planta phenotypes of the microbial strains. Methods of using the microbial strains in field trials are also provided.

公开(公告)号：US20200331820A1

公开(公告)日：2020-10-22

申请号：US16759231

申请日：2018-10-25

申请人： Pivot Bio, Inc.

发明人： Alvin Tamsir ,  Sarah Bloch ,  Neal Shah

IPC分类号： C05F11/08 ,  C12N1/20 ,  C07K14/195 ,  C12N15/52 ,  C12N15/67 ,  C12N15/74 ,  C12Q1/02

摘要： Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.

公开(公告)号：US20220211048A1

公开(公告)日：2022-07-07

申请号：US17605374

申请日：2020-04-24

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Neal Shah ,  Jenny Johnson ,  Bilge Ozaydin Eskiyenenturk

IPC分类号： A01N63/27 ,  C05F11/08 ,  C12N15/52

摘要： A genetically engineered bacterium with a modification in one or more genes selected from: NAC, ptsH, iaaA, gltA, pga, sdiA, fimA1, fimA2, fimA3, fimA4, wzxE, bolA, iscR, fhuF, sodA, sodB, sodC, FNR, arcA, arcB, rpoS, sbnA, treA, treB, phoP, phoQ, yjjPB, ychM, dauA, actP, yusV1, yieL1, yieL2, yieL3, yieL4, pgaB, rafA, melA, uidA, manA, abfA, abnA, lacZ, and yusV2 is disclosed. Methods of use of the genetically engineered bacterium to provide fixed nitrogen to plants, and compositions including the bacterium are also provided.