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    Methods and systems for selection of detection area
    4.
    发明授权
    Methods and systems for selection of detection area 有权

    公开(公告)号：US10436776B2

    公开(公告)日：2019-10-08

    申请号：US15358024

    申请日：2016-11-21

    申请人： PLEXBIO CO., LTD.

    发明人： Yao-Kuang Chung ,  Chia-En Tai ,  Liang Han Chang

    IPC分类号： G01N33/543 ,  G06T7/11

    摘要： Provided herein are methods, systems, devices, and computer-readable storage media for selecting a detection area for a well (e.g., as part of an assay plate) comprising a plurality of encoded microcarriers. In some aspects, selecting the detection area includes obtaining one or more images of the well; calculating a center of the well according to a two-dimensional coordinate system; assigning a position, according to the two-dimensional coordinate system, of a first encoded microcarrier from the plurality; determining whether a distance between the position of the first encoded microcarrier and the center of the well according to the two-dimensional coordinate system exceeds a threshold distance; and including in the detection area one or more microcarriers whose distance from the center of the well does not exceed the threshold distance, while excluding those whose distance from the center of the well exceeds the threshold.

    Methods and systems for image differentiated multiplex assays
    5.
    发明授权
    Methods and systems for image differentiated multiplex assays 有权

    公开(公告)号：US10019815B2

    公开(公告)日：2018-07-10

    申请号：US15460657

    申请日：2017-03-16

    申请人： PLEXBIO CO., LTD.

    发明人： Yao-Kuang Chung ,  Chia-En Tai ,  Chien Teng Kao

    IPC分类号： G06K9/00 ,  G06T7/00

    CPC分类号： G06T7/97 ,  G06T7/0012 ,  G06T7/64 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30072

    摘要： Provided herein are methods, systems, devices, and computer-readable storage media for measuring flatness (e.g., along a Z-axis) of a detection stage. In some aspects, measuring flatness of the detection stage includes obtaining two or more images representing different Z coordinates of a first XY coordinate on a substantially flat substrate positioned on the detection stage; determining a sharpest Z coordinate at the first XY coordinate based on sharpness of the two or more images; obtaining two or more images representing different Z coordinates of a second XY coordinate spaced apart from the first XY coordinate on the substantially flat substrate; determining a sharpest Z coordinate at the second XY coordinate based on sharpness of the two or more images; and calculating a difference between the sharpest Z coordinates at the first and the second XY coordinates to measure the flatness of the detection stage.