公开(公告)号：US07456309B2

公开(公告)日：2008-11-25

申请号：US11014411

申请日：2004-12-16

Applicant: Pradeep Kumar ,  Kailash Chand Gupta

Inventor： Pradeep Kumar ,  Kailash Chand Gupta

IPC: C07C69/67 ,  C07C69/013

CPC classification number: C07H21/00 ,  Y02P20/582

Abstract: The present invention provides a reusable, universal polymer support for repetitive oligonucleotide synthesis and a method of synthesis thereof.

Abstract translation: 本发明提供了一种用于重复寡核苷酸合成的可重用的通用聚合物载体及其合成方法。

公开(公告)号：US5942608A

公开(公告)日：1999-08-24

申请号：US829166

申请日：1997-03-31

Applicant: Pradeep Kumar ,  Kailash Chand Gupta

Inventor： Pradeep Kumar ,  Kailash Chand Gupta

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: A process for preparing oligoribo- or oligodeoxyribonucleotides comprising treating an alkanediol or alkanetriol of formula I ##STR1## wherein R.sup.1 =H--(CH.sub.2).sub.n --; andR.sup.2 =--CH.sub.2 OH or --(CH.sub.2).sub.n --Hn=1-4;with 4,4'-dimethoxytrityl chloride to generate a monosubstituted tritylated compound of formula II ##STR2## wherein R.sup.3 =H--(CH.sub.2).sub.p --; andR.sup.4 =-CH.sub.2 OH or --(CH.sub.2).sub.p --H;R.sup.5 is 4,4'-dimethoxytrityl and concommitantly R.sup.6 is hydrogen, orR.sup.5 is hydrogen and concommitantly R.sup.6 is 4,4'-dimethoxytrityl;p=1-4;and treating the compound of formula II with one equivalent of a homobifunctional alkanedioic acid halide, and contacting the resulting mixture with a polymer support bearing hydroxyl or aminoalkyl functionalities.

Abstract translation: 一种制备寡核苷酸或寡脱氧核糖核苷酸的方法，包括处理式I的链烷二醇或烷三醇，其中R1 = H-（CH2）n-; R2 = -CH2OH或 - （CH2）n-Hn = 1-4; 与4,4'-二甲氧基三苯甲基氯反应以产生式II的单取代三苯甲基化合物，其中R 3 = H-（CH 2）p - ; 和R4 = -CH2OH或 - （CH2）p-H; R5是4,4'-二甲氧基三苯甲基，同时R6是氢，或R5是氢，并且R6是4,4'-二甲氧基三苯甲基; p = 1-4; 并用一当量的同双功能链烷二酰卤处理式II化合物，并将所得混合物与带有羟基或氨基烷基官能团的聚合物载体接触。

公开(公告)号：US08513206B2

公开(公告)日：2013-08-20

申请号：US12181798

申请日：2008-07-29

Applicant: Kailash Chand Gupta ,  Pradeep Kumar ,  Archana Shami ,  Atul Pathak

Inventor： Kailash Chand Gupta ,  Pradeep Kumar ,  Archana Shami ,  Atul Pathak

IPC: A61K48/00 ,  A61K9/50

CPC classification number: A61K31/7088 ,  A61K9/5146 ,  A61K48/00 ,  C08G73/0206 ,  C12N15/87

Abstract: The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.

Abstract translation: 本发明涉及基于新型交联聚乙烯亚胺（PEI）纳米颗粒的核酸转染剂，其中交联剂具有在C2至C8范围内的碳链，范围在3.27-19.8％之间，纳米颗粒的尺寸范围在20-600之间 nm和zeta电位范围为+5至50mV。

公开(公告)号：US20080146462A1

公开(公告)日：2008-06-19

申请号：US11645112

申请日：2006-12-26

Applicant: Kailash Chand Gupta ,  Pradeep Kumar ,  Shweta Mahajan

Inventor： Kailash Chand Gupta ,  Pradeep Kumar ,  Shweta Mahajan

IPC: C40B50/18 ,  C40B50/14

CPC classification number: B01J19/0046 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637

Abstract: The present invention lies in developing a novel method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.

Abstract translation: 本发明在于开发一种制备寡核苷酸微阵列的新方法，其消除了复杂化学反应耗时的缺点，修饰的载体/寡聚体修饰试剂的制备，活化/冷凝试剂的使用，低信噪比 比例，固定化和杂交效率差等。此外，制备的阵列可用于使用杂交测定法检测单核苷酸或多核苷酸错配。

公开(公告)号：US07829505B2

公开(公告)日：2010-11-09

申请号：US11645112

申请日：2006-12-26

Applicant: Kailash Chand Gupta ,  Pradeep Kumar ,  Shweta Mahajan

Inventor： Kailash Chand Gupta ,  Pradeep Kumar ,  Shweta Mahajan

IPC: C40B50/00

CPC classification number: B01J19/0046 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637

Abstract: Disclosed herein is a method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.

Abstract translation: 本文公开了一种制备寡核苷酸微阵列的方法，其消除了复杂化学反应耗时，修饰载体/寡聚体修饰试剂的制备，活化/冷凝试剂的使用，信噪比低，固定不良的缺点 和杂交效率等。此外，制备的阵列可用于使用杂交测定来检测单个或多个核苷酸错配。

公开(公告)号：US06090934A

公开(公告)日：2000-07-18

申请号：US122463

申请日：1998-07-24

Applicant: Pradeep Kumar ,  Kailash Chand Gupta

Inventor： Pradeep Kumar ,  Kailash Chand Gupta

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: A universal polymer support containing an organic aliphatic molecule of structure ##STR1## having at least a pair of cis-hydroxyl groups where one of the hydroxyl groups is attached to the polymer support through a covalent linkage and the other hydroxyl group is protected by an acid labile group.

Abstract translation: 一种通用聚合物载体，其含有具有至少一对顺羟基的结构的有机脂族分子，其中一个羟基通过共价键连接到聚合物载体上，另一个羟基被酸不稳定基团保护。