Abstract:
The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.
Abstract:
The present invention lies in developing a novel method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.
Abstract:
Disclosed herein is a method for the preparation of oligonucleotide microarrays obviating the drawbacks to an extent, such as time consuming complex chemical reactions, preparation of modified supports/oligomer modifying reagents, use of activating/condensing reagent, low signal to noise ratio, poor immobilization and hybridization efficiencies, etc. Further, the prepared arrays can be used to detect single or multiple nucleotide mismatches using hybridization assay.
Abstract:
A universal polymer support containing an organic aliphatic molecule of structure ##STR1## having at least a pair of cis-hydroxyl groups where one of the hydroxyl groups is attached to the polymer support through a covalent linkage and the other hydroxyl group is protected by an acid labile group.
Abstract:
A process for preparing oligoribo- or oligodeoxyribonucleotides comprising treating an alkanediol or alkanetriol of formula I ##STR1## wherein R.sup.1 =H--(CH.sub.2).sub.n --; andR.sup.2 =--CH.sub.2 OH or --(CH.sub.2).sub.n --Hn=1-4;with 4,4'-dimethoxytrityl chloride to generate a monosubstituted tritylated compound of formula II ##STR2## wherein R.sup.3 =H--(CH.sub.2).sub.p --; andR.sup.4 =-CH.sub.2 OH or --(CH.sub.2).sub.p --H;R.sup.5 is 4,4'-dimethoxytrityl and concommitantly R.sup.6 is hydrogen, orR.sup.5 is hydrogen and concommitantly R.sup.6 is 4,4'-dimethoxytrityl;p=1-4;and treating the compound of formula II with one equivalent of a homobifunctional alkanedioic acid halide, and contacting the resulting mixture with a polymer support bearing hydroxyl or aminoalkyl functionalities.
Abstract:
The present invention provides a reusable, universal polymer support for repetitive oligonucleotide synthesis and a method of synthesis thereof.
Abstract:
A medical dictation application configured to operate on a computing device includes a dictation component and a customized operating system (OS) feature in communication with the dictation component, where the customized OS feature and the dictation component utilize a shared authentication process of the medical dictation application, where the customized OS feature is inaccessible to a user device prior to authentication of the user device with the medical dictation application utilizing the shared authentication process.
Abstract:
Systems and methods for suppressing magnetically active surface defects in superconducting quantum circuits are provided. A method includes providing one or more superconducting quantum circuits, and arranging the one or more superconducting quantum circuits in a hermetic enclosure capable of isolating the one or more superconducting circuits from ambient surroundings. The method also includes controlling an environment in the hermetic enclosure to suppress magnetically active surface defects associated with the one or more superconducting quantum circuits. In some aspects, the method further includes introducing an inert gas into the hermetic enclosure to passivate a surface of the one or more superconducting quantum circuits. In other aspects, the method further includes coating a surface of the one or more superconducting circuits with a non-magnetic encapsulation layer. In yet other aspects, the method further includes irradiating the one or more superconducting circuits using ultraviolet light.
Abstract:
A soft card provisioning application is instantiated on a device with wireless communications capabilities. A card number for a soft card desired to be provisioned on the device is obtained from a user of the device. An issuer identification number retrieved from the card number is communicated to a provisioning configuration server over an air interface. A provisioning issuer server network address is obtained from the provisioning configuration server based on the issuer identification number. Card issuer specific challenges are presented to the user, and the user's responses to the challenges are received. The challenge responses are communicated to the provisioning issuer server. Soft card image data and personalization data, where the personalization data includes personalized embossed and pre-printed data, are received from the provisioning issuer server over the air interface. The soft card is provisioned for use on the device based on the data received over the air interface.
Abstract:
Methods, systems, and computer program products for interacting with ISO 14443-4 and MIFARE® applications on the same wireless smart device during a common transaction are disclosed. According to one aspect, the method includes detecting and communicating with an ISO 14443-4 application located on a wireless smart device that includes an ISO 14443-4 and a MIFARE® application to perform at least part of the common transaction. Termination of the ISO 14443-4 application is detected, and the MIFARE® application located on the wireless smart device is detected and communicated with for performing at least part of the common transaction.