公开(公告)号：US5898005A

公开(公告)日：1999-04-27

申请号：US227364

申请日：1994-04-13

申请人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

发明人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

IPC分类号： G01N33/547 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552

CPC分类号： G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/823

摘要： Methods are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. Dendrimer-reagent preparations with particular analyte specificities are mixed in solution with a sample to form dendrimer-reagent-sample complexes. The complexes are then immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供了用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在的方法。 具有特定分析物特异性的树枝状反应剂制剂在溶液中与样品混合以形成树枝状聚合物 - 试剂 - 样品复合物。 然后将络合物固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US6121056A

公开(公告)日：2000-09-19

申请号：US226172

申请日：1994-04-12

申请人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

发明人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/552

CPC分类号： G01N33/552 ,  G01N33/54306 ,  G01N33/545 ,  Y10S436/823

摘要： Methods, compositions and articles of manufacture are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. At least two dendrimer-reagent preparations with different analyte specificities may be immobilized on a solid phase. Alternatively, at least one dendrimer-reagent preparation having multiple specificities may be immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供方法，组合物和制品用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在。 具有不同分析物特异性的至少两种树枝状大分子试剂制剂可以固定在固相上。 或者，至少一种具有多个特异性的树枝状大分子试剂制剂可固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US06083708A

公开(公告)日：2000-07-04

申请号：US514075

申请日：1995-08-11

申请人： Pratap Singh ,  Spencer Lin ,  Fred Moll, III

发明人： Pratap Singh ,  Spencer Lin ,  Fred Moll, III

IPC分类号： C07K16/26 ,  C07K17/08 ,  C12Q1/26 ,  C12Q1/34 ,  G01N33/535 ,  G01N33/543 ,  G01N33/545

CPC分类号： G01N33/535 ,  G01N33/54353 ,  G01N33/545

摘要： Compositions are disclosed, comprising dendrimers to which a first polypeptide is controllably coupled. Such polypeptide-dendrimer compositions are effective for controllably coupling a second polypeptide to the dendrimer. The first and second polypeptides have separate and distinct defined biological activities, for example, two antibodies with first and second binding specificities or an antibody and an enzymatic label. Such compositions are useful as indicators in specific binding assays, e.g., immunoassays. Methods for sequentially coupling two different polypeptides to a dendrimer to form compositions of the invention also are disclosed.

摘要翻译： 公开了包含第一多肽可控制偶联的树枝状大分子的组合物。 这种多肽 - 树枝状大分子组合物对于可控地将第二多肽偶联到树状聚合物是有效的。 第一和第二多肽具有分开且不同的限定的生物活性，例如具有第一和第二结合特异性的两种抗体或抗体和酶标记。 这样的组合物可用作特异性结合测定中的指示剂，例如免疫测定。 还公开了将两种不同多肽顺序偶联到树状聚合物以形成本发明组合物的方法。