公开(公告)号：US06083708A

公开(公告)日：2000-07-04

申请号：US514075

申请日：1995-08-11

申请人： Pratap Singh ,  Spencer Lin ,  Fred Moll, III

发明人： Pratap Singh ,  Spencer Lin ,  Fred Moll, III

IPC分类号： C07K16/26 ,  C07K17/08 ,  C12Q1/26 ,  C12Q1/34 ,  G01N33/535 ,  G01N33/543 ,  G01N33/545

CPC分类号： G01N33/535 ,  G01N33/54353 ,  G01N33/545

摘要： Compositions are disclosed, comprising dendrimers to which a first polypeptide is controllably coupled. Such polypeptide-dendrimer compositions are effective for controllably coupling a second polypeptide to the dendrimer. The first and second polypeptides have separate and distinct defined biological activities, for example, two antibodies with first and second binding specificities or an antibody and an enzymatic label. Such compositions are useful as indicators in specific binding assays, e.g., immunoassays. Methods for sequentially coupling two different polypeptides to a dendrimer to form compositions of the invention also are disclosed.

摘要翻译： 公开了包含第一多肽可控制偶联的树枝状大分子的组合物。 这种多肽 - 树枝状大分子组合物对于可控地将第二多肽偶联到树状聚合物是有效的。 第一和第二多肽具有分开且不同的限定的生物活性，例如具有第一和第二结合特异性的两种抗体或抗体和酶标记。 这样的组合物可用作特异性结合测定中的指示剂，例如免疫测定。 还公开了将两种不同多肽顺序偶联到树状聚合物以形成本发明组合物的方法。

公开(公告)号：US6121056A

公开(公告)日：2000-09-19

申请号：US226172

申请日：1994-04-12

申请人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

发明人： Fred Moll, III ,  Charles Ferzli ,  Spencer H. Lin ,  Pratap Singh

IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/552

CPC分类号： G01N33/552 ,  G01N33/54306 ,  G01N33/545 ,  Y10S436/823

摘要： Methods, compositions and articles of manufacture are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. At least two dendrimer-reagent preparations with different analyte specificities may be immobilized on a solid phase. Alternatively, at least one dendrimer-reagent preparation having multiple specificities may be immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供方法，组合物和制品用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在。 具有不同分析物特异性的至少两种树枝状大分子试剂制剂可以固定在固相上。 或者，至少一种具有多个特异性的树枝状大分子试剂制剂可固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US5898005A

公开(公告)日：1999-04-27

申请号：US227364

申请日：1994-04-13

申请人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

发明人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

IPC分类号： G01N33/547 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552

CPC分类号： G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/823

摘要： Methods are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. Dendrimer-reagent preparations with particular analyte specificities are mixed in solution with a sample to form dendrimer-reagent-sample complexes. The complexes are then immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供了用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在的方法。 具有特定分析物特异性的树枝状反应剂制剂在溶液中与样品混合以形成树枝状聚合物 - 试剂 - 样品复合物。 然后将络合物固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US20110032136A1

公开(公告)日：2011-02-10

申请号：US12512824

申请日：2009-07-30

申请人： Pratap Singh ,  Chandrajit Debnath

发明人： Pratap Singh ,  Chandrajit Debnath

IPC分类号： H03M1/12 ,  H03K5/22

CPC分类号： H03K3/356139 ,  H03K3/013

摘要： The present disclosure relates to reduction in the effect of kickback in comparators by means of charge injection implemented by means of voltage controlled switches with attributes similar to those of an input differential pair. The voltage controlled switches produce charge to neutralize the charge loss during latching of inputs in the comparator.

摘要翻译： 本公开涉及通过借助于具有与输入差分对的属性类似的电压控制开关实现的电荷注入来减少比较器中反冲的影响。 电压控制开关产生电荷以中和比较器中的输入锁存期间的电荷损失。

公开(公告)号：US20090258435A1

公开(公告)日：2009-10-15

申请号：US12103245

申请日：2008-04-15

申请人： Sandra A. Lewisch ,  Pratap Singh ,  Viral Desai ,  Karen L. Krakowski ,  James E. Duffy

发明人： Sandra A. Lewisch ,  Pratap Singh ,  Viral Desai ,  Karen L. Krakowski ,  James E. Duffy

IPC分类号： G01N33/566 ,  G01N33/536

CPC分类号： G01N33/532 ,  G01N33/54393 ,  G01N33/566

摘要： Methods are disclosed for designing an antibody reagent for use in an assay for the detection of an analyte to obtain an optimum assay sensitivity and/or dynamic range. The antibody reagent is a conjugate of a small molecule attached by a spacer group to an antibody for the analyte. The method comprises controlling, in the preparation of the conjugate, reaction parameters comprising the hydrophobicity or hydrophilicity of the spacer group, the length of the spacer group, the number of molecules of the small molecule attached to the antibody and the point of attachment of the small molecule to the antibody to obtain an optimum assay sensitivity and/or dynamic range. In some embodiments the method comprises preparing two or more conjugates by selecting a set of parameters for each conjugate wherein the set of parameters is different for each conjugate, conducting an assay for the analyte employing each conjugate and selecting for use in the assay the conjugate that provides the optimum assay sensitivity and/or dynamic range.

摘要翻译： 公开了设计用于分析物检测以获得最佳测定灵敏度和/或动态范围的测定中的抗体试剂的方法。 抗体试剂是通过间隔基团与分析物的抗体连接的小分子的缀合物。 该方法包括在共轭物的制备中控制包括间隔基团的疏水性或亲水性，间隔基团的长度，与抗体连接的小分子的分子数目和 小分子到抗体以获得最佳测定灵敏度和/或动态范围。 在一些实施方案中，该方法包括通过为每个缀合物选择一组参数来制备两种或多种缀合物，其中该组参数对于每个缀合物是不同的，使用每个缀合物进行分析物的测定，并选择用于测定缀合物 提供最佳测定灵敏度和/或动态范围。

公开(公告)号：US20080074190A1

公开(公告)日：2008-03-27

申请号：US11900917

申请日：2007-09-12

申请人： Pratap Singh ,  Chandrajit Debnath ,  Rakesh Malik ,  Arnold D'Souza

发明人： Pratap Singh ,  Chandrajit Debnath ,  Rakesh Malik ,  Arnold D'Souza

IPC分类号： H03F3/26 ,  H03F1/22

CPC分类号： H03F3/45188 ,  H03F3/45677 ,  H03F2203/45052 ,  H03F2203/45074 ,  H03F2203/45248 ,  H03F2203/45296 ,  H03F2203/45302 ,  H03F2203/45318 ,  H03F2203/45342 ,  H03F2203/45356

摘要： Embodiments of the present invention disclose operational amplifiers which demonstrate good settling behavior with minimum over-shoot or ringing for improving settling behavior. The amplifiers include one or more amplification stages connected to form a symmetric structure. The amplification stage includes a boosting amplifier, a MOS transistor and a compensation capacitor. The MOS transistor can be an NMOS transistor and a PMOS transistor. Using this scheme pole-zero doublets are rearranged in a manner to improve the transient settling response.

摘要翻译： 本发明的实施例公开了运行放大器，其以最小的过冲或振铃来表现出良好的沉降行为，以改善沉降行为。 放大器包括一个或多个连接以形成对称结构的放大级。 放大级包括升压放大器，MOS晶体管和补偿电容器。 MOS晶体管可以是NMOS晶体管和PMOS晶体管。 使用该方案，极点双重重排以改善瞬态稳定响应的方式。

公开(公告)号：US06248353B1

公开(公告)日：2001-06-19

申请号：US09459137

申请日：1999-12-10

申请人： Pratap Singh

发明人： Pratap Singh

IPC分类号： A61K3514

CPC分类号： G01N33/86 ,  A61K9/1271 ,  A61K9/1278 ,  Y02A50/473 ,  Y10S436/829

摘要： The present application relates to a method of making liposomes having membrane proteins incorporated therein, the method comprising: providing the membrane protein in solution; providing a solution of preformed liposomes; and incubating the mixture. Prior to the step of providing a solution of preformed liposomes, the liposomes are formed by combining a mixture of phospholipids with a solution of at least one type of unsaturated fatty acid. The methods of the present invention further relate to the method of making a reagent comprising tissue factor reconstituted into preformed liposomes. The method of the present invention for making a tissue factor reagent comprises: providing tissue factor in solution; providing a solution of preformed liposomes comprising a mixture of phospholipids and at least one type of unsaturated fatty acid; and incubating the mixture.

摘要翻译： 本申请涉及一种制备其中掺入膜蛋白的脂质体的方法，所述方法包括：在溶液中提供膜蛋白; 提供预先形成的脂质体的溶液; 并孵育混合物。 在提供预先形成的脂质体的溶液的步骤之前，通过将磷脂的混合物与至少一种类型的不饱和脂肪酸的溶液组合而形成脂质体。 本发明的方法还涉及制备包含重构成预先形成的脂质体的组织因子的试剂的方法。 本发明的制备组织因子试剂的方法包括：在溶液中提供组织因子; 提供包含磷脂和至少一种不饱和脂肪酸的混合物的预先形成的脂质体的溶液; 并孵育混合物。

公开(公告)号：US5861319A

公开(公告)日：1999-01-19

申请号：US331243

申请日：1994-10-28

申请人： Spencer H. Lin ,  Kwok Sum Yu ,  Pratap Singh ,  Steven E. Diamond

发明人： Spencer H. Lin ,  Kwok Sum Yu ,  Pratap Singh ,  Steven E. Diamond

IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/552 ,  G01N33/78

CPC分类号： G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/817 ,  Y10S436/823 ,  Y10S530/811

摘要： Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by covalently coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供了用于特异性结合试验的方法和组合物，其中特异性结合试剂固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物共价偶联来促进固定。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US08768898B1

公开(公告)日：2014-07-01

申请号：US11740471

申请日：2007-04-26

申请人： Don Alvin Trimmer ,  Sandeep Yadav ,  Pratap Singh

发明人： Don Alvin Trimmer ,  Sandeep Yadav ,  Pratap Singh

IPC分类号： G06F17/30

CPC分类号： G06F17/30115 ,  G06F17/30138 ,  G06F17/302

摘要： A method and system for performing data manipulation on a storage device is disclosed. A data manipulation command is created on a computing device, wherein the computing device is separate from the storage device. The computing device is a client or a server that requests services of a storage system to store data on a storage medium. The computing device and the storage device are connected over a network. The computing device executes a host application, and its data is stored on the medium. The computing device issues a command to the storage device to be performed on the data. The storage device executes the command and sends the result to the computing device. As a result, the data is not sent to the computing device for manipulation.

摘要翻译： 公开了一种用于在存储设备上执行数据操作的方法和系统。 在计算设备上创建数据操作命令，其中所述计算设备与所述存储设备分离。 计算设备是请求存储系统的服务以在存储介质上存储数据的客户机或服务器。 计算设备和存储设备通过网络连接。 计算设备执行主机应用，其数据存储在介质上。 计算设备向存储设备发出要对数据执行的命令。 存储装置执行命令并将结果发送到计算装置。 结果，数据不发送到计算设备进行操作。

公开(公告)号：US08546148B2

公开(公告)日：2013-10-01

申请号：US13442503

申请日：2012-04-09

申请人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

发明人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

IPC分类号： G01N21/76 ,  G01N33/53 ,  C08F226/06 ,  C08F226/10 ,  C08F220/58

CPC分类号： C09D133/26 ,  C08F120/60 ,  C08F126/10 ,  C08F220/06 ,  C08F220/26 ,  C08F220/38 ,  C08F220/58 ,  C08F2800/20 ,  C09D139/06 ,  C09D177/06 ,  C09D179/04 ,  C09D181/00 ,  G01N33/533 ,  G01N33/582

摘要： A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

摘要翻译： 用作测定试剂的组合物包括固体支持物，其包括信号产生系统的成员和合成共聚物的涂层。 合成共聚物包含第一聚合单体，其包括包含反应性官能团或反应性官能团的衍生物的侧基部分，和包含包含至少1个碳原子和至少2个杂原子的侧链部分的第二聚合单体。 在一些实施方案中，共聚物包含包含侧链部分的聚乙烯主链，其包含反应性官能团或反应性官能团的衍生物，并且所述侧链部分包含至少1个碳原子和至少2个杂原子。