公开(公告)号：US09632085B2

公开(公告)日：2017-04-25

申请号：US14382043

申请日：2013-02-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Donald E. Ingber ,  Mark J. Cartwright ,  Alexander Watters ,  John Samuel Workman ,  Daniel Levner ,  Martin Marcus Rottman

IPC: G01N33/53 ,  G01N33/569 ,  C12Q1/18

CPC classification number: G01N33/56938 ,  C12Q1/18 ,  G01N33/56911 ,  G01N33/56916 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2500/10

Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.

公开(公告)号：US11807677B2

公开(公告)日：2023-11-07

申请号：US17552662

申请日：2021-12-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  G01N33/569 ,  C07K14/00 ,  C07K14/47 ,  C07K19/00 ,  A61K38/00

CPC classification number: C07K14/78 ,  C07K14/00 ,  C07K14/4726 ,  C07K19/00 ,  G01N33/569 ,  A61K38/00 ,  C07K2319/30 ,  G01N2333/78 ,  G01N2400/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e g, enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US10435457B2

公开(公告)日：2019-10-08

申请号：US15750788

申请日：2016-08-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  C07K14/00 ,  C07K19/00 ,  G01N33/569 ,  C07K14/47 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e.g., enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US20190077850A1

公开(公告)日：2019-03-14

申请号：US16059799

申请日：2018-08-09

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Michael Super ,  Alexander Watters

IPC: C07K16/12 ,  G01N33/487 ,  G01N33/569 ,  G01N33/543 ,  A61P31/04

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. The microbe-targeting or microbe-binding molecules can comprise a microbe surface-binding domain linked to a portion of an Fc region. Further, the microbe-targeting molecules can be conjugated to substrate (e.g., a magnetic particle) to form a microbe-targeting substrate. Such microbe-targeting molecules and/or substrates and the kits comprising the same can be used in various applications, such as diagnosis and/or treatment of an infection caused by microbes. Moreover, the microbe-targeting molecules and/or substrates can be easily regenerated after use.

公开(公告)号：US11236149B2

公开(公告)日：2022-02-01

申请号：US16879015

申请日：2020-05-20

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  G01N33/569 ,  C07K14/00 ,  C07K14/47 ,  C07K19/00 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e.g., enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US10696733B2

公开(公告)日：2020-06-30

申请号：US16553635

申请日：2019-08-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  G01N33/569 ,  C07K14/00 ,  C07K14/47 ,  C07K19/00 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e.g., enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US20150064703A1

公开(公告)日：2015-03-05

申请号：US14382043

申请日：2013-02-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Donald E. Ingber ,  Mark J. Cartwright ,  Alexander Watters ,  John Samuel Workman ,  Daniel Levner ,  Martin Rottman

IPC: G01N33/569

CPC classification number: G01N33/56938 ,  C12Q1/18 ,  G01N33/56911 ,  G01N33/56916 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2500/10

Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.

Abstract translation: 本文描述的各个方面的实施方案涉及用于在收集样品后几小时内快速测定微生物的抗生素敏感性的方法，组合物，试剂盒和系统。 在一些实施方案中，本文所述的方法，组合物，试剂盒和系统可以允许基于少量微生物测定微生物的抗生素敏感性，例如与本文所述的微生物靶向底物结合的少至5-10微克。