公开(公告)号：US09791440B2

公开(公告)日：2017-10-17

申请号：US14904583

申请日：2014-07-15

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Mark J. Cartwright ,  Nazita Gamini ,  Donald E. Ingber ,  Martin Rottman ,  Michael Super ,  Julie A. Tomolonis ,  Karen A. Sinclair

IPC: A61K39/02 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/569 ,  C12Q1/18

CPC classification number: G01N33/56911 ,  C12Q1/18 ,  G01N33/5695 ,  G01N33/56961 ,  G01N2333/4724 ,  G01N2400/00 ,  G01N2800/26 ,  G01N2800/52

Abstract: The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

公开(公告)号：US11795212B2

公开(公告)日：2023-10-24

申请号：US17093781

申请日：2020-11-10

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin Rottman ,  Alexander L. Watters

IPC: C07K16/12 ,  A61K47/68 ,  G01N33/543 ,  G01N33/569 ,  C07K14/42

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  C07K2319/33 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/30

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：US10718766B2

公开(公告)日：2020-07-21

申请号：US15668794

申请日：2017-08-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Mark J. Cartwright ,  Nazita Gamini ,  Donald E. Ingber ,  Martin Rottman ,  Michael Super ,  Julie A. Tomolonis ,  Karen A. Sinclair

IPC: A61K39/00 ,  A61K39/02 ,  C12Q1/00 ,  G01N33/569 ,  C12Q1/18

Abstract: The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

公开(公告)号：US11718651B2

公开(公告)日：2023-08-08

申请号：US17318343

申请日：2021-05-12

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander L. Watters ,  Donald E. Ingber ,  Mark J. Cartwright ,  Michael Super ,  Martin Rottman ,  Evangelia Murray ,  Brendon Dusel

IPC: C07K14/47 ,  G01N33/68 ,  G01N33/569 ,  A61K47/42 ,  A61K38/00

CPC classification number: C07K14/4737 ,  A61K47/42 ,  C07K14/4726 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56944 ,  G01N33/6854 ,  A61K38/00 ,  C07K2319/30 ,  C07K2319/33 ,  G01N2333/4737

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.

公开(公告)号：US11209432B2

公开(公告)日：2021-12-28

申请号：US16898754

申请日：2020-06-11

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Mark J. Cartwright ,  Nazita Gamini ,  Donald E. Ingber ,  Martin Rottman ,  Michael Super ,  Julie A. Tomolonis ,  Karen A. Sinclair

IPC: A61K39/00 ,  A61K39/02 ,  G01N33/569 ,  C12Q1/18

Abstract: The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

公开(公告)号：US20150064703A1

公开(公告)日：2015-03-05

申请号：US14382043

申请日：2013-02-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Donald E. Ingber ,  Mark J. Cartwright ,  Alexander Watters ,  John Samuel Workman ,  Daniel Levner ,  Martin Rottman

IPC: G01N33/569

CPC classification number: G01N33/56938 ,  C12Q1/18 ,  G01N33/56911 ,  G01N33/56916 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2500/10

Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.

Abstract translation: 本文描述的各个方面的实施方案涉及用于在收集样品后几小时内快速测定微生物的抗生素敏感性的方法，组合物，试剂盒和系统。 在一些实施方案中，本文所述的方法，组合物，试剂盒和系统可以允许基于少量微生物测定微生物的抗生素敏感性，例如与本文所述的微生物靶向底物结合的少至5-10微克。

公开(公告)号：US11034744B2

公开(公告)日：2021-06-15

申请号：US16669805

申请日：2019-10-31

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander L. Watters ,  Donald E. Ingber ,  Mark J. Cartwright ,  Michael Super ,  Martin Rottman ,  Evangelia Murray ,  Brendon Dusel

IPC: C07K14/47 ,  G01N33/68 ,  G01N33/569 ,  A61K47/42 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.

公开(公告)号：US10551379B2

公开(公告)日：2020-02-04

申请号：US14766575

申请日：2014-03-14

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Mark J. Cartwright ,  Martin M. Rottman ,  Julie A. Tomolonis

IPC: G01N33/50 ,  G01N33/569

Abstract: Methods, compositions, kits and systems for detecting and/or capturing a target entity in a sample are disclosed. In particular, the methods, compositions and kits described herein can be used for pretreatment of target-binding agents with a blocking agent to reduce non-target binding in a complex matrix (e.g., blood). Methods and compositions for detecting and/or capturing a microbe in a test sample, including bodily fluids such as blood and tissues of a subject, food, water, and environmental surfaces are also disclosed.

公开(公告)号：US10526399B2

公开(公告)日：2020-01-07

申请号：US15415352

申请日：2017-01-25

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin Rottman ,  Alexander L. Watters

IPC: C07K16/12 ,  G01N33/543 ,  G01N33/569 ,  C07K14/42 ,  A61K47/68

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：US10513546B2

公开(公告)日：2019-12-24

申请号：US15105298

申请日：2014-12-18

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander L. Watters ,  Donald E. Ingber ,  Mark J. Cartwright ,  Michael Super ,  Martin Rottman ,  Evangelia Murray ,  Brendon Dusel

IPC: C07K14/47 ,  G01N33/569 ,  A61K47/42 ,  G01N33/68 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.