公开(公告)号：US20250129408A1

公开(公告)日：2025-04-24

申请号：US18989169

申请日：2024-12-20

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Je-Hyuk Lee ,  George M. Church ,  Michael Super

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N33/53

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

公开(公告)号：US11919971B2

公开(公告)日：2024-03-05

申请号：US16302023

申请日：2017-05-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Daniel Christopher Leslie ,  Thomas Doyle ,  Anna Waterhouse ,  Melissa Rodas ,  Alexander L. Watters ,  Michael Super ,  Donald E. Ingber

IPC: C07K17/08 ,  C08L25/06 ,  C08L83/04 ,  G01N33/543 ,  G01N33/545 ,  C08K5/05

CPC classification number: C07K17/08 ,  C08L25/06 ,  C08L83/04 ,  G01N33/54393 ,  G01N33/545 ,  C08K5/05 ,  C08L2203/02

Abstract: This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.

公开(公告)号：US11807677B2

公开(公告)日：2023-11-07

申请号：US17552662

申请日：2021-12-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  G01N33/569 ,  C07K14/00 ,  C07K14/47 ,  C07K19/00 ,  A61K38/00

CPC classification number: C07K14/78 ,  C07K14/00 ,  C07K14/4726 ,  C07K19/00 ,  G01N33/569 ,  A61K38/00 ,  C07K2319/30 ,  G01N2333/78 ,  G01N2400/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e g, enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US11639518B2

公开(公告)日：2023-05-02

申请号：US17584959

申请日：2022-01-26

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

Abstract: Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US20220195017A1

公开(公告)日：2022-06-23

申请号：US17565783

申请日：2021-12-30

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Michael Super ,  Alexander L. Watters

IPC: C07K16/12 ,  G01N33/487 ,  A61P31/04 ,  G01N33/543 ,  G01N33/569 ,  C07K16/14 ,  C07K14/705

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. The microbe-targeting or microbe-binding molecules can comprise a microbe surface-binding domain linked to a portion of an Fc region. Further, the microbe-targeting molecules can be conjugated to substrate (e.g., a magnetic particle) to form a microbe-targeting substrate. Such microbe-targeting molecules and/or substrates and the kits comprising the same can be used in various applications, such as diagnosis and/or treatment of an infection caused by microbes. Moreover, the microbe-targeting molecules and/or substrates can be easily regenerated after use.

公开(公告)号：US20220170077A1

公开(公告)日：2022-06-02

申请号：US17671803

申请日：2022-02-15

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837

Abstract: Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US10538562B2

公开(公告)日：2020-01-21

申请号：US15839352

申请日：2017-12-12

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

IPC: C07K14/77 ,  C07K17/00 ,  C07K16/44 ,  G01N33/569 ,  C07K14/47

Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.

公开(公告)号：US11549136B2

公开(公告)日：2023-01-10

申请号：US17366127

申请日：2021-07-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US11312949B2

公开(公告)日：2022-04-26

申请号：US16661595

申请日：2019-10-23

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Alexander L. Watters ,  Philip T. Snell ,  Donald E. Ingber

IPC: C12N9/96 ,  C12N9/26 ,  A61K38/00 ,  C07K14/805

Abstract: Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.

公开(公告)号：US11059873B2

公开(公告)日：2021-07-13

申请号：US16702724

申请日：2019-12-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

IPC: C07K14/47 ,  G01N33/569 ,  C07K16/44 ,  C07K17/00 ,  G01N33/50 ,  G01N27/74

Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.