公开(公告)号：US20200095704A1

公开(公告)日：2020-03-26

申请号：US16513945

申请日：2019-07-17

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Francois Vigneault ,  Uri Laserson ,  Ido Bachelet

IPC分类号： C40B10/00 ,  C12Q1/6881 ,  C12N15/10 ,  C07K16/00 ,  C40B30/04 ,  C40B40/08 ,  G16B30/00 ,  G16B20/00 ,  C12Q1/6806

摘要： Methods and compositions for determining and/or monitoring the immune state of an individual are provided.

公开(公告)号：US12012670B2

公开(公告)日：2024-06-18

申请号：US16513945

申请日：2019-07-17

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Francois Vigneault ,  Uri Laserson ,  Ido Bachelet

IPC分类号： C12N15/10 ,  C07K16/00 ,  C07K16/06 ,  C12Q1/6806 ,  C12Q1/6881 ,  C12Q1/6883 ,  C40B10/00 ,  C40B30/04 ,  C40B40/08 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/6874

CPC分类号： C40B10/00 ,  C07K16/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B30/04 ,  C40B40/08 ,  G16B20/00 ,  G16B30/00 ,  C07K16/065 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2535/00 ,  C12Q2535/122 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q1/6883 ,  C12Q2535/122 ,  C12Q1/6881 ,  C12Q2535/122

摘要： This disclosure provides methods for monitoring an immune response. Methods comprise linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained before an immune response and linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained during or after an immune response. Methods further comprise performing high-throughput sequencing of the linked (paired) sequences from before the immune response and from during or after the immune response, and comparing the resulting sequence reads.