公开(公告)号：US20240191211A1

公开(公告)日：2024-06-13

申请号：US18312487

申请日：2023-05-04

申请人： Promega Corporation

发明人： Rachel Friedman Ohana ,  Ce Shi ,  Robin Hurst ,  Mark A. Klein ,  Jian Cao ,  Thomas Kirkland ,  Connor Fitzgerald ,  Tetsuo Uyeda ,  Thomas Machleidt ,  Wenhui Zhou ,  Matthew A. Larsen ,  Hui Wang ,  Weiwei An ,  Karilyn Porter

IPC分类号： C12N9/02 ,  B01J31/22 ,  B01J35/39

CPC分类号： C12N9/0069 ,  B01J31/2295 ,  B01J35/39 ,  C12Y113/12013

摘要： Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

公开(公告)号：US20140287427A1

公开(公告)日：2014-09-25

申请号：US14204868

申请日：2014-03-11

申请人： Promega Corporation

发明人： Carolyn W. Hitko ,  Robin Hurst ,  Thomas Kirkland ,  Dieter Klaubert ,  Poncho Meisenheimer ,  Nidhi Nath ,  Rachel Friedman Ohana ,  Paul Otto ,  Marjeta Urh ,  Tetsuo Uyeda ,  Keith Wood

IPC分类号： G01N33/569

CPC分类号： G01N33/543 ,  G01N33/5005

摘要： The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

摘要翻译： 本发明提供用于捕获和鉴定生物活性剂的细胞靶标的组合物和方法。 特别地，本文提供的是用于捕获配体，细胞靶标（任选地用报告物标记），捕获蛋白质（任选地作为捕获二聚体存在），表面（例如显示，捕获配体，捕获蛋白质或捕获二聚体）的生物活性剂， 以及捕获和鉴定生物活性剂的细胞靶标的方法。