公开(公告)号：US10233483B2

公开(公告)日：2019-03-19

申请号：US14901200

申请日：2014-07-03

Applicant: Qvella Corporation

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/68 ,  C12Q1/689

Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

公开(公告)号：US12247197B2

公开(公告)日：2025-03-11

申请号：US18012433

申请日：2021-06-25

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Anna Khimchenko ,  Maryam Asadishekari ,  Stephen Wesley Leonard

IPC: G06K9/62 ,  C12N1/20 ,  G02B21/36 ,  G06T5/50 ,  G06T5/70 ,  G06V10/147 ,  G06V10/774 ,  G06V10/82 ,  G06V20/69

Abstract: Systems and methods are provided for classifying microbial cells according to morphological features of microcolonies. A dark-field objective is employed to acquire a dark-field image of a microcolony during a microcolony growth phase that is characterized by phenotypic expression of microcolony morphological features which evolve with time and are differentiated among classes of microbial cell types. The dark-field image is processed to classify the microcolony according to two or more microbial cell types, such as Gram status and/or speciation. The dark-field objective may have a numerical aperture selected to facilitate the imaging of microcolony morphological features, residing, for example, between 0.15 and 0.35. A set of dark-field images of a microcolony may be collected during the microcolony growth phase and processed to classify the microcolony. Classification may be performed according to a temporal ordering of the dark-field images, for example, using a recurrent neural network.

公开(公告)号：US20190177766A1

公开(公告)日：2019-06-13

申请号：US16280842

申请日：2019-02-20

Applicant: Qvella Corporation

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/689

Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

公开(公告)号：US20160138072A1

公开(公告)日：2016-05-19

申请号：US14901200

申请日：2014-07-03

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/68

CPC classification number: C12Q1/18 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/158

Abstract: Methods are provided for performing antibotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the pressence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

Abstract translation: 提供了基于在暴露于抗生素后从微生物细胞中检测RNA（例如tmRNA）进行抗生素敏感性测试的方法。 在一些实施方案中，从样品获得等分试样，其中一个样品含有选定的抗生素。 包括生长培养基在内的等分试样在适合于微生物生长的条件下孵育，并除去每个等分试样中的微生物细胞并裂解，并将裂解物进行逆转录和扩增，推断所选抗生素对微生物的作用 细胞。 在一个实施方案中，将含有微生物细胞的样品在所选择的抗生素的压力下孵育，并提供刺激以诱导在微生物细胞内的m RNA上产生。 随后裂解微生物细胞，而不会在裂解物中明显降解m RNA，并检测m RNA以确定抗生素对微生物细胞的作用。

公开(公告)号：US11866762B2

公开(公告)日：2024-01-09

申请号：US17240093

申请日：2021-04-26

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/689

CPC classification number: C12Q1/18 ,  C12Q1/689 ,  C12Q2600/106 ,  C12Q2600/158

Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

公开(公告)号：US20210246483A1

公开(公告)日：2021-08-12

申请号：US17240093

申请日：2021-04-26

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/689

Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

公开(公告)号：US10988794B2

公开(公告)日：2021-04-27

申请号：US16280842

申请日：2019-02-20

Applicant: Qvella Corporation

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

IPC: C12Q1/18 ,  C12Q1/68 ,  C12Q1/689

Abstract: Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production of m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.