公开(公告)号：US06375819B1

公开(公告)日：2002-04-23

申请号：US09373909

申请日：1999-08-13

申请人： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

发明人： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

IPC分类号： G01N2726

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US06365024B1

公开(公告)日：2002-04-02

申请号：US09372151

申请日：1999-08-11

申请人： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

发明人： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

IPC分类号： G01N2726

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US6027627A

公开(公告)日：2000-02-22

申请号：US105988

申请日：1998-06-29

申请人： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

发明人： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/477

CPC分类号： G01N27/44782

摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

摘要翻译： 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。