公开(公告)号：US20170044582A1

公开(公告)日：2017-02-16

申请号：US14603032

申请日：2015-01-22

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： David H. Sherman ,  Michael Marie Kaufman-Schofield ,  Sunit Jain ,  Gregory Dick

IPC: C12P17/18 ,  C12N9/10 ,  C12N9/88 ,  C12N9/02

CPC classification number: C12P17/185 ,  C12N9/0051 ,  C12N9/0095 ,  C12N9/1007 ,  C12N9/88 ,  C12Y108/01004 ,  C12Y118/01002 ,  C12Y402/01001

Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Abstract translation: 本公开涉及非核糖体肽合成酶（NRPS）衍生的药物及其类似物的生物合成途径。 本发明提供了用于合成来自NRPS的药物的方便的微生物宿主中的异源表达的多核苷酸序列，由这些多核苷酸编码的多肽，包含多核苷酸的表达载体，包含多核苷酸或表达载体的宿主细胞，以及包含 宿主细胞。 还提供了用于生产来自NRPS的药物ET-743的方法。

公开(公告)号：US09994885B2

公开(公告)日：2018-06-12

申请号：US14603032

申请日：2015-01-22

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： David H. Sherman ,  Michael Marie Kaufman-Schofield ,  Sunit Jain ,  Gregory Dick

IPC: C12P17/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12P17/185 ,  C12N9/0051 ,  C12N9/0095 ,  C12N9/1007 ,  C12N9/88 ,  C12Y108/01004 ,  C12Y118/01002 ,  C12Y402/01001

Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

公开(公告)号：US20150361470A1

公开(公告)日：2015-12-17

申请号：US14713662

申请日：2015-05-15

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： David H. Sherman ,  Michael Marie Kaufman-Schofield ,  Sunit Jain ,  Gregory Dick

IPC: C12P17/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12N9/10

CPC classification number: C12N9/1029 ,  C12N9/0006 ,  C12N9/0051 ,  C12N9/0095 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P17/185 ,  C12Y108/01007 ,  C12Y118/01002 ,  C12Y201/01 ,  C12Y203/01 ,  C12Y203/0104 ,  C12Y203/01187 ,  C12Y402/01001 ,  C12Y402/01059 ,  C12Y603/04015

Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Abstract translation: 本公开涉及来自非核糖体肽合成酶（NRPS）的药物及其类似物的生物合成途径。 本发明提供了用于合成来自NRPS的药物的方便的微生物宿主中的异源表达的多核苷酸序列，由这些多核苷酸编码的多肽，包含多核苷酸的表达载体，包含多核苷酸或表达载体的宿主细胞，以及包含 宿主细胞。 还提供了用于生产来自NRPS的药物ET-743的方法。

公开(公告)号：US09487763B2

公开(公告)日：2016-11-08

申请号：US14713662

申请日：2015-05-15

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： David H. Sherman ,  Michael Marie Kaufman-Schofield ,  Sunit Jain ,  Gregory Dick

IPC: C12P17/18 ,  C12N9/10 ,  C12N9/00 ,  C12N9/88 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52

CPC classification number: C12N9/1029 ,  C12N9/0006 ,  C12N9/0051 ,  C12N9/0095 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P17/185 ,  C12Y108/01007 ,  C12Y118/01002 ,  C12Y201/01 ,  C12Y203/01 ,  C12Y203/0104 ,  C12Y203/01187 ,  C12Y402/01001 ,  C12Y402/01059 ,  C12Y603/04015

Abstract: The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Abstract translation: 本公开涉及来自非核糖体肽合成酶（NRPS）的药物及其类似物的生物合成途径。 本发明提供了用于合成来自NRPS的药物的方便的微生物宿主中的异源表达的多核苷酸序列，由这些多核苷酸编码的多肽，包含多核苷酸的表达载体，包含多核苷酸或表达载体的宿主细胞，以及包含 宿主细胞。 还提供了用于生产来自NRPS的药物ET-743的方法。