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公开(公告)号：US20110318801A1
公开(公告)日：2011-12-29
申请号：US13161749
申请日：2011-06-16
申请人： ROBEL Y. KAHSAY , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
发明人： ROBEL Y. KAHSAY , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
摘要： Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.
摘要翻译： 发现来自A. missouriensis的表达木糖异构酶的发酵单胞菌在含有木糖的培养基中生长时具有改善的木糖利用率，生长和乙醇生产。 通过分子系统发育和轮廓隐马尔可夫模型分析，结构地鉴定了与密螺旋体相关的木糖异构酶，提供可用于改善木糖利用的木糖异构酶。
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公开(公告)号：US08623623B2
公开(公告)日：2014-01-07
申请号：US13161749
申请日：2011-06-16
申请人： Robel Y. Kahsay , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
发明人： Robel Y. Kahsay , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
IPC分类号： C12N9/00
摘要： Zymomonas expressing xylose isomerase from A. missouriensis was found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that of A. missouriensis were identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.
摘要翻译： 发现来自A. missouriensis的表达木糖异构酶的发酵单胞菌在含有木糖的培养基中生长时具有改善的木糖利用率，生长和乙醇生产。 通过分子系统发育和轮廓隐马尔可夫模型分析，结构地鉴定了与密螺旋体相关的木糖异构酶，提供可用于改善木糖利用的木糖异构酶。
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公开(公告)号：US07998722B2
公开(公告)日：2011-08-16
申请号：US12410501
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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公开(公告)号：US20130078694A1
公开(公告)日：2013-03-28
申请号：US13433343
申请日：2012-03-29
申请人： LUAN TAO , Jean-Francois Tomb , Paul V. Viitanen
发明人： LUAN TAO , Jean-Francois Tomb , Paul V. Viitanen
CPC分类号： C12N15/74 , C12N9/0006 , C12N9/88 , C12P7/06 , C12P7/065 , C12P13/02 , C12Y101/01169 , C12Y401/01011 , Y02E50/17
摘要： Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.
摘要翻译： 发酵单胞菌不能合成泛酸，在生长培养基中需要这种必需的维生素。 用用于表达2-脱氢山梨醇酯还原酶和天冬氨酸1-脱羧酶的操纵子转化的发酵单胞菌菌株能够在不含泛酸的培养基中生长。 这些菌株可用于在种子培养和发酵培养基中不添加泛酸的乙醇生产。
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公开(公告)号：US07989206B2
公开(公告)日：2011-08-02
申请号：US12410495
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
CPC分类号： C12N15/74
摘要： Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译： 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。
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6.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07803623B2
公开(公告)日：2010-09-28
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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公开(公告)号：US08765426B2
公开(公告)日：2014-07-01
申请号：US13433343
申请日：2012-03-29
申请人： Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
发明人： Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
CPC分类号： C12N15/74 , C12N9/0006 , C12N9/88 , C12P7/06 , C12P7/065 , C12P13/02 , C12Y101/01169 , C12Y401/01011 , Y02E50/17
摘要： Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.
摘要翻译： 发酵单胞菌不能合成泛酸，在生长培养基中需要这种必需的维生素。 用用于表达2-脱氢山梨醇酯还原酶和天冬氨酸1-脱羧酶的操纵子转化的发酵单胞菌菌株能够在不含泛酸的培养基中生长。 这些菌株可用于在种子培养和发酵培养基中不添加泛酸的乙醇生产。
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公开(公告)号：US08679822B2
公开(公告)日：2014-03-25
申请号：US13161734
申请日：2011-06-16
申请人： Perry G. Caimi , Laura McCole , Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
发明人： Perry G. Caimi , Laura McCole , Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
摘要： Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
摘要翻译： 发现木糖利用的发酵单胞菌菌株在含木糖培养基中生长时积累核酮糖。 工程化这些菌株以增加核糖-5-磷酸异构酶活性，导致核酮糖积累减少，生长改善，木糖利用率提高，乙醇生产增加。
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公开(公告)号：US20120156746A1
公开(公告)日：2012-06-21
申请号：US13161734
申请日：2011-06-16
申请人： Perry G. Caimi , Laura McCole , Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
发明人： Perry G. Caimi , Laura McCole , Luan Tao , Jean-Francois Tomb , Paul V. Viitanen
摘要： Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
摘要翻译： 发现木糖利用的发酵单胞菌菌株在含木糖培养基中生长时积累核酮糖。 工程化这些菌株以增加核糖-5-磷酸异构酶活性，导致核酮糖积累减少，生长改善，木糖利用率提高，乙醇生产增加。
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10.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07897396B2
公开(公告)日：2011-03-01
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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