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公开(公告)号：US07998722B2
公开(公告)日：2011-08-16
申请号：US12410501
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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公开(公告)号：US07989206B2
公开(公告)日：2011-08-02
申请号：US12410495
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
CPC分类号： C12N15/74
摘要： Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译： 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。
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3.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07803623B2
公开(公告)日：2010-09-28
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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4.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07897396B2
公开(公告)日：2011-03-01
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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5.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090221078A1
公开(公告)日：2009-09-03
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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6.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090203099A1
公开(公告)日：2009-08-13
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
IPC分类号： C12P7/06
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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7.发明授权Alpha(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof 有权α（1,6）连接的葡萄糖寡糖水解酶多核苷酸和多肽及其使用方法公开(公告)号：US07268221B2
公开(公告)日：2007-09-11
申请号：US10648152
申请日：2003-08-25
申请人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan Nigarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan Nigarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译： 本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。 一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。 通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。
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8.发明授权α(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof 失效α（1,6）连接的葡萄糖寡糖水解酶多核苷酸和多肽及其使用方法公开(公告)号：US07615365B2
公开(公告)日：2009-11-10
申请号：US11852834
申请日：2007-09-10
申请人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译： 本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。 一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。 通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。
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公开(公告)号：US20090068704A1
公开(公告)日：2009-03-12
申请号：US11852834
申请日：2007-09-10
申请人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
IPC分类号： C12N15/31 , C12N15/52 , C07K14/00 , C12N15/63 , C12P7/20 , C12P1/04 , C12P19/04 , C12P1/02 , C12P7/00 , C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译： 本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。 一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。 通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。
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