Quantitative determination of nucleic acid amplification products

    公开(公告)号:US06573054B2

    公开(公告)日:2003-06-03

    申请号:US10043415

    申请日:2002-01-10

    IPC分类号: C12Q168

    摘要: The present invention relates to a method for detecting the amount of a target polynucleotide in a sample. A combination is provided in a medium. The combination comprises (i) a sample suspected of containing the target polynucleotide, the target polynucleotide being in single stranded form, (ii) a reference polynucleotide comprising a sequence that is common with a sequence of the target polynucleotide, and (iii) a predetermined amount of an oligonucleotide probe that has a sequence that hybridizes with the sequence that is common. The combination is subjected to conditions for amplifying the target polynucleotide and the reference polynucleotide. The conditions permit formation of substantially non-dissociative complexes of the target polynucleotide and the reference polynucleotide, respectively, with the oligonucleotide probe. Furthermore, the predetermined amount of the oligonucleotide probe is less than the expected amount of the amplified target polynucleotide. The ratio of the amount of the complex of the target polynucleotide with the oligonucleotide probe to the amount of the complex of the reference polynucleotide with the oligonucleotide probe is determined. Determination of the ratio is facilitated by employing second and third oligonucleotide probes. The second oligonucleotide probe has a sequence that hybridizes only with the second sequence of the target polynucleotide. The third oligonucleotide probe has a sequence that hybridizes only with a respective second sequence of the reference polynucleotide. The ratio is related to the known amount of the reference polynucleotide to determine the amount of the target polynucleotide in the sample. One or more reference polynucleotides may be employed with a corresponding third oligonucleotide probe for each reference probe. Kits for carrying out the above methods are also disclosed. The method is particularly applicable to the amplification and detection of RNA.

    Quantitative determination of nucleic acid amplification products

    公开(公告)号:US06365346B1

    公开(公告)日:2002-04-02

    申请号:US09025639

    申请日:1998-02-18

    IPC分类号: C12Q168

    摘要: The present invention relates to a method for detecting the amount of a target polynucleotide in a sample. A combination is provided in a medium. The combination comprises (i) a sample suspected of containing the target polynucleotide, the target polynucleotide being in single stranded form, (ii) a reference polynucleotide comprising a sequence that is common with a sequence of the target polynucleotide, and (iii) a predetermined amount of an oligonucleotide probe that has a sequence that hybridizes with the sequence that is common. The combination is subjected to conditions for amplifying the target polynucleotide and the reference polynucleotide. The conditions permit formation of substantially non-dissociative complexes of the target polynucleotide and the reference polynucleotide, respectively, with the oligonucleotide probe. Furthermore, the predetermined amount of the oligonucleotide probe is less than the expected amount of the amplified target polynucleotide. The ratio of the amount of the complex of the target polynucleotide with the oligonucleotide probe to the amount of the complex of the reference polynucleotide with the oligonucleotide probe is determined. Determination of the ratio is facilitated by employing second and third oligonucleotide probes. The second oligonucleotide probe has a sequence that hybridizes only with the second sequence of the target polynucleotide. The third oligonucleotide probe has a sequence that hybridizes only with a respective second sequence of the reference polynucleotide. The ratio is related to the known amount of the reference polynucleotide to determine the amount of the target polynucleotide in the sample. One or more reference polynucleotides may be employed with a corresponding third oligonucleotide probe for each reference probe. Kits for carrying out the above methods are also disclosed. The method is particularly applicable to the amplification and detection of RNA.

    System and method for client interoperability
    3.
    发明授权
    System and method for client interoperability 有权
    客户端互操作性的系统和方法

    公开(公告)号:US08316083B2

    公开(公告)日:2012-11-20

    申请号:US12400633

    申请日:2009-03-09

    IPC分类号: G06F15/16 G06F15/173 G06F3/00

    摘要: Disclosed herein is a system and method for client interoperability. In one embodiment, a method for client interoperability comprises establishing a network connection between a client and a proxy. The client requests a connection factory from the proxy. The proxy then obtains the connection factory from an application server using a Java Naming and Directory Interface (JNDI). Using the connection factory, a connection to a connection host from the client is created. When the client sends a message, the proxy receives the message and routes the message to a destination server. Similarly, when the client receives a message, the proxy receives the message from a destination server and passes the message to the client. The client can be specifically configured to extend JMS operability to a desired language. In one embodiment the client is a .NET client.

    摘要翻译: 本文公开了一种用于客户端互操作性的系统和方法。 在一个实施例中,用于客户端互操作性的方法包括在客户端和代理之间建立网络连接。 客户端从代理请求一个连接工厂。 代理然后使用Java命名和目录接口(JNDI)从应用程序服务器获取连接工厂。 使用连接工厂,创建与客户端连接主机的连接。 当客户端发送消息时,代理接收消息并将消息路由到目标服务器。 类似地,当客户端接收到消息时,代理从目的地服务器接收消息,并将消息传递给客户端。 客户端可以专门配置为将JMS的可操作性扩展到所需的语言。 在一个实施例中,客户端是.NET客户端。

    SYSTEM AND METHOD FOR CLIENT INTEROPERABILITY
    7.
    发明申请
    SYSTEM AND METHOD FOR CLIENT INTEROPERABILITY 有权
    用于客户互操作性的系统和方法

    公开(公告)号:US20090259715A1

    公开(公告)日:2009-10-15

    申请号:US12400633

    申请日:2009-03-09

    IPC分类号: G06F15/16 G06F9/44

    摘要: Disclosed herein is a system and method for client interoperability. In one embodiment, a method for client interoperability comprises establishing a network connection between a client and a proxy. The client requests a connection factory from the proxy. The proxy then obtains the connection factory from an application server using a Java Naming and Directory Interface (JNDI). Using the connection factory, a connection to a connection host from the client is created. When the client sends a message, the proxy receives the message and routes the message to a destination server. Similarly, when the client receives a message, the proxy receives the message from a destination server and passes the message to the client. The client can be specifically configured to extend JMS operability to a desired language. In one embodiment the client is a .NET client.

    摘要翻译: 本文公开了一种用于客户端互操作性的系统和方法。 在一个实施例中,用于客户端互操作性的方法包括在客户端和代理之间建立网络连接。 客户端从代理请求一个连接工厂。 代理然后使用Java命名和目录接口(JNDI)从应用程序服务器获取连接工厂。 使用连接工厂,创建与客户端连接主机的连接。 当客户端发送消息时,代理接收消息并将消息路由到目标服务器。 类似地,当客户端接收到消息时,代理从目的地服务器接收消息,并将消息传递给客户端。 客户端可以专门配置为将JMS的可操作性扩展到所需的语言。 在一个实施例中,客户端是.NET客户端。

    IMMINENT COLLISION WARNING SYSTEM AND METHOD
    8.
    发明申请
    IMMINENT COLLISION WARNING SYSTEM AND METHOD 审中-公开
    立即碰撞警告系统和方法

    公开(公告)号:US20090134988A1

    公开(公告)日:2009-05-28

    申请号:US12268958

    申请日:2008-11-11

    IPC分类号: B60Q9/00

    CPC分类号: G08G1/164

    摘要: The invention is directed to methods and systems for sensing the presence of objects in the intended path of an automobile with a system to warn both the operator of the vehicle and the object of regard.

    摘要翻译: 本发明涉及用于感测汽车预期路径中物体的存在的方法和系统,其具有警告车辆的操作者和所关注的对象的系统。

    Method for intelligent parking/pollution and surveillance control system
    9.
    发明授权
    Method for intelligent parking/pollution and surveillance control system 有权
    智能停车/污染监控系统方法

    公开(公告)号:US07321317B2

    公开(公告)日:2008-01-22

    申请号:US11478510

    申请日:2006-06-29

    IPC分类号: G08G1/01

    摘要: The invention relates to systems to manage parking meters using mechanical, electrical and electromechanicals monitoring and transmitting devices. Such intelligent parking meters may be used for example to monitor pollution by idling vehicles, survey the area about the meters to identify events such as vandalism, accident or any other designated emergency situation, and to monitor volume and flow of traffic to help coordinate traffic light sequencing for facilitating optimum traffic movement. The meters may be coin, bill or card operated and are useful for monitoring individual parking spaces designed to monitor conditions ambient to the parking meter and issue summons to vehicles violating the preset requirements.

    摘要翻译: 本发明涉及使用机械,电气和机电监控和发射装置来管理停车计费器的系统。 这样的智能停车收费表可以用于监测怠速车辆的污染,调查仪表的区域,以识别事故,如破坏行为,事故或任何其他指定的紧急情况,并监测交通量和流量,以协助交通灯 排序,以促进最佳交通运动。 仪表可以是硬币,纸币或卡片操作,并且可用于监测专用于监视停车计时器周围环境的各个停车位,并向违反预设要求的车辆发出传票。

    Apparatus and method for dynamically clocking a loop filter in a digital communications device
    10.
    发明申请
    Apparatus and method for dynamically clocking a loop filter in a digital communications device 有权
    用于在数字通信设备中动态计时环路滤波器的装置和方法

    公开(公告)号:US20070104289A1

    公开(公告)日:2007-05-10

    申请号:US11268798

    申请日:2005-11-08

    IPC分类号: H04L27/00

    CPC分类号: H04L27/12 H04L27/20

    摘要: A transmitter has a signal generator, an amplifier, a detection circuit, a comparison circuit, a loop filter, and an adjustable clock. The signal generator produces a signal. The signal is produced with a first frequency characteristic and contains frequency-related information. The detection circuit detects the first frequency-related characteristic and generates an associated signal in response. A comparison circuit compares the signal from the detection circuit and another signal. It outputs a signal associated with the difference between the two. A loop filter receives the output of the comparison circuit and generates a signal to the signal generator in. The loop filter is clocked at a second frequency by a signal from a clock circuit. The clock circuit can compare the first frequency and the second frequency, and can change the second frequency based upon a relationship between the two frequencies.

    摘要翻译: 发射机具有信号发生器,放大器,检测电路,比较电路,环路滤波器和可调节时钟。 信号发生器产生信号。 该信号产生具有第一频率特性并且包含频率相关信息。 检测电路检测第一频率相关特性并产生响应的相关信号。 比较电路将来自检测电路的信号与另一信号进行比较。 它输出与两者之间的差异相关联的信号。 环路滤波器接收比较电路的输出并产生信号到信号发生器。环路滤波器以来自时钟电路的信号以第二频率被计时。 时钟电路可以比较第一频率和第二频率,并且可以基于两个频率之间的关系来改变第二频率。