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    LIGHT-REGULATED PROMOTERS
    3.
    发明申请
    LIGHT-REGULATED PROMOTERS 审中-公开
    光调节促销

    公开(公告)号：US20120131691A1

    公开(公告)日：2012-05-24

    申请号：US13322136

    申请日：2010-05-17

    申请人： Peter P. Repetti ,  Rajnish Khanna ,  Hans E. Holtan ,  T. Lynne Reuber ,  Oliver J. Ratcliffe

    发明人： Peter P. Repetti ,  Rajnish Khanna ,  Hans E. Holtan ,  T. Lynne Reuber ,  Oliver J. Ratcliffe

    IPC分类号： A01H5/00 ,  A01H1/06 ,  A01H1/02 ,  C12N15/82

    CPC分类号： C12N15/8202

    摘要： Light-regulated promoter sequences were identified that respond to differential light conditions and so can be used to regulate gene expression in a light- or dark-inducible manner. These promoters may be used to produce transgenic plants that have an altered trait relative to control plants. In preferred embodiments, the transgenic plants with the improved traits are morphologically and/or developmentally similar to control plants (examples of the latter include wild-type or non-transformed plants of the same species). Any of these light-regulated promoters may be incorporated into a nucleic acid construct that comprises a polynucleotide regulated by one such promoter and that encodes a polypeptide or RNA molecule that, when ectopically expressed, confers an improved trait in plants.

    摘要翻译： 鉴定了响应差异光条件的光调节启动子序列，因此可以以浅或暗诱导的方式用于调节基因表达。 这些启动子可用于产生相对于对照植物具有改变的性状的转基因植物。 在优选的实施方案中，具有改善的性状的转基因植物在形态上和/或发育上与对照植物相似（后者的实例包括相同物种的野生型或非转化植物）。 这些光调节启动子中的任何一个可以掺入到包含由一个这样的启动子调控的多核苷酸的核酸构建体中，并且编码多肽或RNA分子，所述多肽或RNA分子当异位表达时赋予植物中改良的性状。

    STRONG ACTIVATION DOMAIN
    5.
    发明申请
    STRONG ACTIVATION DOMAIN 审中-公开
    强活动域

    公开(公告)号：US20110126326A1

    公开(公告)日：2011-05-26

    申请号：US13000488

    申请日：2009-06-26

    申请人： Shiv Tiwari ,  Roger Canales ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver Ratcliffe

    发明人： Shiv Tiwari ,  Roger Canales ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver Ratcliffe

    IPC分类号： A01H5/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07H21/00 ,  C12N5/10 ,  C12N15/82

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    摘要翻译： 从拟南芥蛋白质乙烯响应因子98（AtERF98）中鉴定出新的和强的转录激活结构域。 该领域已经被指定为“EDLL结构域”，并且具有许多高度保守的氨基酸残基，其在来自植物的AtERF98家族的整个成员中发现，包括单子叶植物和雌蕊直系同源物。 当EDLL结构域与植物和酵母的转录因子融合时，显示其具有高度的活性，并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。 当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时，EDLL结构域也是活性的。 与其他已知的活化结构域（如VP16和GAL4）不同，EDLL结构域的大小相对较小，并且是植物来源的，因此作为在转基因食品作物中应用的强转录激活工具是有利的。