公开(公告)号：US20050063051A1

公开(公告)日：2005-03-24

申请号：US10888186

申请日：2004-07-09

申请人： Ralph Lange ,  Stefan Wilhelm ,  Volker Gerstner ,  Roland Scheler ,  Michael Goelles ,  Joerg-Michael Funk

发明人： Ralph Lange ,  Stefan Wilhelm ,  Volker Gerstner ,  Roland Scheler ,  Michael Goelles ,  Joerg-Michael Funk

IPC分类号： G02B21/18 ,  G02B21/00 ,  G02B21/06

CPC分类号： G02B21/002 ,  G02B21/06

摘要： An arrangement for direct input coupling of a laser, preferably of a short-pulse laser, for nonlinear sample excitation which is located outside of the microscope housing, into the beam path of a laser scanning microscope (LSM), comprising a housing in which is integrated an adjusting laser that can be coupled into the microscope beam path by beam splitters, wherein the housing is advantageously integrated so as to be insertable in the scan module of the LSMA, and a beam splitter for the direct coupling laser has high transmission in the direction of the objective and, further, partial transmission for back-reflections of the adjusting laser coming from the specimen for adjusting the overlapping of the adjusting laser and direct coupling laser.

摘要翻译： 用于直接输入耦合激光扫描显微镜（LSM）的激光扫描显微镜（LSM）的光束路径中的用于将位于显微镜外壳之外的非线性样品激发的短脉冲激光器的激光直接输入耦合的装置，其中， 集成了可以通过分束器耦合到显微镜光束路径中的调节激光器，其中壳体有利地集成以便可插入到LSMA的扫描模块中，并且用于直接耦合激光器的分束器在 并且进一步地，用于调整来自测试样品的调节激光器的反射反射的部分透射以调节调节激光器和直接耦合激光器的重叠。

公开(公告)号：US07656583B2

公开(公告)日：2010-02-02

申请号：US11783289

申请日：2007-04-06

申请人： Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

发明人： Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

IPC分类号： G02B27/10

CPC分类号： G02B27/144 ,  G02B21/0064

摘要： A beam corradiator for combining two radiation beams, preferably movable beams independent from each other in at least one direction, to scan and/or influence a sample, preferably a manipulation system and an imaging system, with a partially reflecting layer being provided for the corradiation, wherein the thickness of the layer is provided with a preferably consistent incline or decline over the optically effective cross-section of the beam corradiatior.

摘要翻译： 一种用于组合两个辐射束，优选在至少一个方向上彼此独立的可移动光束以扫描和/或影响样品，优选地操纵系统和成像系统的光束校正器，其中提供了部分反射层用于辐照 ，其中所述层的厚度在所述光束辐射体的光学有效横截面上被提供优选地一致的倾斜或下降。

公开(公告)号：US20120268749A1

公开(公告)日：2012-10-25

申请号：US13299515

申请日：2011-11-18

申请人： Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

发明人： Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

IPC分类号： G01B11/14

CPC分类号： G01B11/14 ,  G02B21/0036 ,  G02B21/008

摘要： Laser scanning microscope and its operating method in which at least two first and second scanning systems activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, The scanning fields created by the light distributions on the sample mutually overlap to create a reference pattern on the sample with one of the light distributions, which is then captured and used to create the overlap using the second light distribution and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined.

摘要翻译： 激光扫描显微镜及其操作方法，其中至少两个第一和第二扫描系统彼此独立地激活并且可以在至少一个方向上移动，借助于光束组合元件照射样品，并且光被检测到 样品进入，由样品上的光分布产生的扫描场相互重叠，以在样品上产生参考图案，其中一个光分布被捕获并用于使用第二光分布来创建重叠 和/或布置在采样平面或中间图像平面中的参考图案由两个扫描场捕获并用于创建样本的重叠和/或结构特征由两个扫描场作为参考图案捕获，并用于 创建确定校正值的重叠。

公开(公告)号：US20070159689A1

公开(公告)日：2007-07-12

申请号：US10586137

申请日：2005-03-24

申请人： Dieter Schau ,  Peter Huehn ,  Michael Goelles ,  Joerg-Michael Funk

发明人： Dieter Schau ,  Peter Huehn ,  Michael Goelles ,  Joerg-Michael Funk

IPC分类号： G02B21/00

CPC分类号： G02B21/002 ,  G02B21/248

摘要： Arrangement for changing the uncoupling of the object beam and/or the coupling of the beam in a Laser Scanning Microscope, whereby a rotatable tube revolver is provided, which has at least four switching positions and each tube position is associated with a separate tube lens as well as deflecting elements for the object beam, whereby the tube positions for at least the LSM operation, LSM/NDD and/or manipulation and/or illumination, camera and visual operation modes are provided.

摘要翻译： 用于在激光扫描显微镜中改变物体束的解耦和/或光束的耦合的装置，由此提供可旋转的管旋转枪，其具有至少四个切换位置，并且每个管位置与单独的管透镜相关联 以及用于物体光束的偏转元件，由此提供用于至少LSM操作，LSM / NDD和/或操纵和/或照明，照相机和视觉操作模式的管位置。

公开(公告)号：US20090296207A1

公开(公告)日：2009-12-03

申请号：US11783255

申请日：2007-04-06

申请人： Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

发明人： Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

IPC分类号： G02B21/06

CPC分类号： G01B11/14 ,  G02B21/0036 ,  G02B21/008

摘要： Laser scanning microscope and its operating method in which at least two first and second light distributions activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, characterized by the fact that the scanning fields created by the light distributions on the sample are made to overlap mutually such that a reference pattern is created on the sample with one of the light distributions, which is then captured and used to create the overlap with the help of the second light distribution (correction values are determined) and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap (correction values are determined) and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined.

摘要翻译： 激光扫描显微镜及其操作方法，其中至少两个第一和第二光分布彼此独立地激活并且可以在至少一个方向上移动，借助于光束组合元件照射样品，并且光被 样品进入，其特征在于使由样品上的光分布产生的扫描场相互重叠，使得在具有一个光分布的样品上产生参考图案，然后被捕获， 用于通过第二光分布（校正值被确定）和/或布置在采样平面中或中间图像平面中的参考图案在两个扫描场捕获并用于创建重叠（校正 值被确定）和/或样本的结构特征被两个扫描场作为参考图案捕获并用于创建重叠 其中确定校正值。

公开(公告)号：US20080130125A1

公开(公告)日：2008-06-05

申请号：US11783289

申请日：2007-04-06

申请人： Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

发明人： Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

IPC分类号： G02B27/10

CPC分类号： G02B27/144 ,  G02B21/0064

摘要： A beam corradiator for combining two radiation beams, preferably movable beams independent from each other in at least one direction, to scan and/or influence a sample, preferably a manipulation system and an imaging system, with a partially reflecting layer being provided for the corradiation, wherein the thickness of the layer is provided with a preferably consistent incline or decline over the optically effective cross-section of the beam corradiatior.

摘要翻译： 一种用于组合两个辐射束，优选在至少一个方向上彼此独立的可移动光束以扫描和/或影响样品，优选地操纵系统和成像系统的光束校正器，其中提供了部分反射层用于辐照 ，其中所述层的厚度在所述光束辐射体的光学有效横截面上被提供优选地一致的倾斜或下降。

公开(公告)号：US20070131875A1

公开(公告)日：2007-06-14

申请号：US11590851

申请日：2006-11-01

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0048

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scamming means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过所耦合的第一和第二扫描装置的移动进行图像采集，并且通过样品的照射进行三维采样运动，其中第二打扫装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。

公开(公告)号：US20060011858A1

公开(公告)日：2006-01-19

申请号：US10967328

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0048

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过被耦合的第一和第二扫描装置的运动而进行图像采集，并且三维采样运动是通过样品的照射完成的，其中第二扫描装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。

公开(公告)号：US07649683B2

公开(公告)日：2010-01-19

申请号：US10967341

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G02B21/06

CPC分类号： G02B21/008 ,  G02B21/0036 ,  Y10S359/90

摘要： Process for observing at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a line and are detected simultaneously with a spatially resolving detector. At an angle to the plane of the relative movement, a second scanner is moved and an image acquisition takes place by coupling the movement of the first and second scanners and a three-dimensional sampling movement being done by the illumination of the sample. The second scanner is coupled to the movement of the first scanner such that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.

摘要翻译： 使用光栅显微镜通过第一扫描仪沿着基本上垂直于照明轴的至少一个扫描轴的照射光和样本之间的相对运动来观察至少一个样本区域的过程，其中几个照亮的采样点位于一条线上，并且是 与空间分辨检测器同时检测。 与相对运动的平面成一角度，第二扫描器被移动，并且通过耦合第一和第二扫描器的运动以及通过样本的照明来完成三维采样运动来进行图像采集。 第二扫描器耦合到第一扫描仪的运动，使得扫描直线和/或曲线和/或平面和/或曲面，其沿着第一扫描器的至少一个扫描方向以及沿着扫描 第二扫描仪的方向。

公开(公告)号：US20080285123A1

公开(公告)日：2008-11-20

申请号：US11987219

申请日：2007-11-28

申请人： Joerg-Michael Funk ,  Ralf Wolleschensky ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Ralf Engelmann

发明人： Joerg-Michael Funk ,  Ralf Wolleschensky ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Ralf Engelmann

IPC分类号： G02B21/06

CPC分类号： G02B21/002 ,  G02B21/0032

摘要： Raster scanning light microscope with line pattern scanning with at least one illumination module, in which the means to achieve a variable partition of the laser light into least two illumination channels are envisioned and joint illumination of a sample takes place at the same or at different areas of the sample.

摘要翻译： 具有至少一个照明模块的线阵扫描的光栅扫描光学显微镜，其中实现将激光可变分割成至少两个照明通道的装置，并且在相同或不同的区域发生样品的联合照明 的样品。