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公开(公告)号：US20120268749A1
公开(公告)日：2012-10-25
申请号：US13299515
申请日：2011-11-18
申请人： Michael Goelles , Ralf Netz , Frank Hecht , Joerg-Michael Funk
发明人： Michael Goelles , Ralf Netz , Frank Hecht , Joerg-Michael Funk
IPC分类号： G01B11/14
CPC分类号： G01B11/14 , G02B21/0036 , G02B21/008
摘要： Laser scanning microscope and its operating method in which at least two first and second scanning systems activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, The scanning fields created by the light distributions on the sample mutually overlap to create a reference pattern on the sample with one of the light distributions, which is then captured and used to create the overlap using the second light distribution and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined.
摘要翻译： 激光扫描显微镜及其操作方法，其中至少两个第一和第二扫描系统彼此独立地激活并且可以在至少一个方向上移动，借助于光束组合元件照射样品，并且光被检测到 样品进入，由样品上的光分布产生的扫描场相互重叠，以在样品上产生参考图案，其中一个光分布被捕获并用于使用第二光分布来创建重叠 和/或布置在采样平面或中间图像平面中的参考图案由两个扫描场捕获并用于创建样本的重叠和/或结构特征由两个扫描场作为参考图案捕获，并用于 创建确定校正值的重叠。
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公开(公告)号：US20090296207A1
公开(公告)日：2009-12-03
申请号：US11783255
申请日：2007-04-06
申请人： Michael Goelles , Ralf Netz , Frank Hecht , Joerg-Michael Funk
发明人： Michael Goelles , Ralf Netz , Frank Hecht , Joerg-Michael Funk
IPC分类号： G02B21/06
CPC分类号： G01B11/14 , G02B21/0036 , G02B21/008
摘要： Laser scanning microscope and its operating method in which at least two first and second light distributions activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, characterized by the fact that the scanning fields created by the light distributions on the sample are made to overlap mutually such that a reference pattern is created on the sample with one of the light distributions, which is then captured and used to create the overlap with the help of the second light distribution (correction values are determined) and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap (correction values are determined) and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined.
摘要翻译： 激光扫描显微镜及其操作方法，其中至少两个第一和第二光分布彼此独立地激活并且可以在至少一个方向上移动，借助于光束组合元件照射样品，并且光被 样品进入，其特征在于使由样品上的光分布产生的扫描场相互重叠，使得在具有一个光分布的样品上产生参考图案，然后被捕获， 用于通过第二光分布（校正值被确定）和/或布置在采样平面中或中间图像平面中的参考图案在两个扫描场捕获并用于创建重叠（校正 值被确定）和/或样本的结构特征被两个扫描场作为参考图案捕获并用于创建重叠 其中确定校正值。
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公开(公告)号：US07656583B2
公开(公告)日：2010-02-02
申请号：US11783289
申请日：2007-04-06
IPC分类号： G02B27/10
CPC分类号： G02B27/144 , G02B21/0064
摘要： A beam corradiator for combining two radiation beams, preferably movable beams independent from each other in at least one direction, to scan and/or influence a sample, preferably a manipulation system and an imaging system, with a partially reflecting layer being provided for the corradiation, wherein the thickness of the layer is provided with a preferably consistent incline or decline over the optically effective cross-section of the beam corradiatior.
摘要翻译： 一种用于组合两个辐射束，优选在至少一个方向上彼此独立的可移动光束以扫描和/或影响样品，优选地操纵系统和成像系统的光束校正器，其中提供了部分反射层用于辐照 ，其中所述层的厚度在所述光束辐射体的光学有效横截面上被提供优选地一致的倾斜或下降。
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公开(公告)号：US20080130125A1
公开(公告)日：2008-06-05
申请号：US11783289
申请日：2007-04-06
IPC分类号： G02B27/10
CPC分类号： G02B27/144 , G02B21/0064
摘要： A beam corradiator for combining two radiation beams, preferably movable beams independent from each other in at least one direction, to scan and/or influence a sample, preferably a manipulation system and an imaging system, with a partially reflecting layer being provided for the corradiation, wherein the thickness of the layer is provided with a preferably consistent incline or decline over the optically effective cross-section of the beam corradiatior.
摘要翻译： 一种用于组合两个辐射束，优选在至少一个方向上彼此独立的可移动光束以扫描和/或影响样品，优选地操纵系统和成像系统的光束校正器，其中提供了部分反射层用于辐照 ，其中所述层的厚度在所述光束辐射体的光学有效横截面上被提供优选地一致的倾斜或下降。
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5.发明申请METHOD AND ARRANGEMENT FOR CONTROLLED ACTUATION OF A MICROSCOPE, IN PARTICULAR OF A LASER SCANNING MICROSCOPE 有权用于控制微波激光的方法和装置，特别是激光扫描显微镜公开(公告)号：US20100097694A1
公开(公告)日：2010-04-22
申请号：US12578202
申请日：2009-10-13
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/00 , G02B21/0032 , G02B21/0076 , G02B27/0972
摘要： Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light.
摘要翻译： 显微镜，特别是激光扫描显微镜的致动控制方法，其中至少一个至少在一个方向上移动的第一照明光以及至少沿一个方向移动的至少一个第二照明光， 通过光束组合器照射样品，发生来自样品的光的检测，由此通过从公共照明单元分离光而产生照明光的至少一部分，其特征在于，通过装置 发生公共控制单元的受控分割，进入第一和第二照明光，其中由用户指定或自动指定的第一照明光的强度被分配为比较高的优先级（优先化） 对于第二照明光的指定值，并且进行第二照明光的调整，直到获得最大值，其由v 为第一个照明灯指定。
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6.发明申请Method and arrangement for the controlled actuation of a microscope, in particular of a laser scanning microscope 审中-公开用于显微镜的受控致动的方法和装置，特别是激光扫描显微镜公开(公告)号：US20080068709A1
公开(公告)日：2008-03-20
申请号：US11783290
申请日：2007-04-06
IPC分类号： G02B21/06
CPC分类号： G02B21/06 , G02B21/00 , G02B21/0032 , G02B21/0076 , G02B27/0972
摘要： Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combination, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light.
摘要翻译： 显微镜，特别是激光扫描显微镜的致动控制方法，其中至少一个至少在一个方向上移动的第一照明光以及至少沿一个方向移动的至少一个第二照明光， 通过光束组合照射样品，发生来自样品的光的检测，由此通过从公共照明单元分离光而产生照明光的至少一部分，其特征在于，通过装置 发生公共控制单元的受控分割，进入第一和第二照明光，其中由用户指定或自动指定的第一照明光的强度被分配为比较高的优先级（优先化） 对于第二照明光的指定值，并且进行第二照明光的调整，直到获得由th确定的最大值 为第一个照明灯指定的e值。
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7.发明申请Tube-type revolver with at least four positions for injecting or extracting light into or from a laser scanning microscope 审中-公开具有至少四个位置的管式左轮手枪，用于将光从激光扫描显微镜注入或提取公开(公告)号：US20070159689A1
公开(公告)日：2007-07-12
申请号：US10586137
申请日：2005-03-24
申请人： Dieter Schau , Peter Huehn , Michael Goelles , Joerg-Michael Funk
发明人： Dieter Schau , Peter Huehn , Michael Goelles , Joerg-Michael Funk
IPC分类号： G02B21/00
CPC分类号： G02B21/002 , G02B21/248
摘要： Arrangement for changing the uncoupling of the object beam and/or the coupling of the beam in a Laser Scanning Microscope, whereby a rotatable tube revolver is provided, which has at least four switching positions and each tube position is associated with a separate tube lens as well as deflecting elements for the object beam, whereby the tube positions for at least the LSM operation, LSM/NDD and/or manipulation and/or illumination, camera and visual operation modes are provided.
摘要翻译： 用于在激光扫描显微镜中改变物体束的解耦和/或光束的耦合的装置，由此提供可旋转的管旋转枪，其具有至少四个切换位置，并且每个管位置与单独的管透镜相关联 以及用于物体光束的偏转元件，由此提供用于至少LSM操作，LSM / NDD和/或操纵和/或照明，照相机和视觉操作模式的管位置。
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8.发明申请Arrangement for the direct inout coupling of a laser, particularly of a short-pulse laser 审中-公开用于激光器，特别是短脉冲激光器的直接入射耦合的布置公开(公告)号：US20050063051A1
公开(公告)日：2005-03-24
申请号：US10888186
申请日：2004-07-09
申请人： Ralph Lange , Stefan Wilhelm , Volker Gerstner , Roland Scheler , Michael Goelles , Joerg-Michael Funk
发明人： Ralph Lange , Stefan Wilhelm , Volker Gerstner , Roland Scheler , Michael Goelles , Joerg-Michael Funk
CPC分类号： G02B21/002 , G02B21/06
摘要： An arrangement for direct input coupling of a laser, preferably of a short-pulse laser, for nonlinear sample excitation which is located outside of the microscope housing, into the beam path of a laser scanning microscope (LSM), comprising a housing in which is integrated an adjusting laser that can be coupled into the microscope beam path by beam splitters, wherein the housing is advantageously integrated so as to be insertable in the scan module of the LSMA, and a beam splitter for the direct coupling laser has high transmission in the direction of the objective and, further, partial transmission for back-reflections of the adjusting laser coming from the specimen for adjusting the overlapping of the adjusting laser and direct coupling laser.
摘要翻译： 用于直接输入耦合激光扫描显微镜（LSM）的激光扫描显微镜（LSM）的光束路径中的用于将位于显微镜外壳之外的非线性样品激发的短脉冲激光器的激光直接输入耦合的装置，其中， 集成了可以通过分束器耦合到显微镜光束路径中的调节激光器，其中壳体有利地集成以便可插入到LSMA的扫描模块中，并且用于直接耦合激光器的分束器在 并且进一步地，用于调整来自测试样品的调节激光器的反射反射的部分透射以调节调节激光器和直接耦合激光器的重叠。
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9.发明申请Process for the observation of at least one sample region with a light raster microscope 有权用光栅显微镜观察至少一个样品区域的方法公开(公告)号：US20070131875A1
公开(公告)日：2007-06-14
申请号：US11590851
申请日：2006-11-01
申请人： Ralf Engelmann , Joerg-Michael Funk , Joerg Steinert , Bernhard Zimmermann , Stefan Wilhelm , Joerg Engel , Ulrich Meisel
发明人： Ralf Engelmann , Joerg-Michael Funk , Joerg Steinert , Bernhard Zimmermann , Stefan Wilhelm , Joerg Engel , Ulrich Meisel
IPC分类号： G01N21/64
CPC分类号： G02B21/0076 , G02B21/0048
摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scamming means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.
摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过所耦合的第一和第二扫描装置的移动进行图像采集，并且通过样品的照射进行三维采样运动，其中第二打扫装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。
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公开(公告)号：US20060012785A1
公开(公告)日：2006-01-19
申请号：US10967330
申请日：2004-10-19
CPC分类号： G01J3/06 , G01J3/02 , G01J3/0202 , G01J3/021 , G01J3/0227 , G01J3/0237 , G01J3/10 , G01J3/44 , G01J2003/1217 , G02B21/0044 , G02B21/0064 , G02B21/0076 , G02B21/008
摘要： In a confocal laser scanning microscope for Raman spectroscopy with an illuminating configuration (2), which provides an illuminating beam for illuminating a probe region (23), with a scanning configuration (3, 4), which guides the illuminating beam over the probe while scanning, and with a detector configuration (5), which via the scanning configuration (3, 4) images the illuminated probe region (23) by means of a confocal aperture (26) on to at least one detector unit (28), it is provided that the illuminating configuration (2) of the scanning configuration (3, 4) provides a line-shaped illuminating beam, that the scanning configuration (3, 4) guides the line-shaped illuminating beam over the probe f while scanning and that the confocal aperture is designed as a slotted aperture (26) or as a slot-shaped region (28, 48) of the detector unit (28) acting as a confocal aperture.
摘要翻译： 在具有照明配置（2）的拉曼光谱的共焦激光扫描显微镜中，其提供用于照射探针区域（23）的照明光束，其具有扫描配置（3,4），其将照射光束引导到探针上，同时 扫描和检测器配置（5），其经由扫描配置（3,4）通过共焦孔（26）将照射的探测区域（23）图像到至少一个检测器单元（28）上， 提供扫描配置（3,4）的照明配置（2）提供线形照明光束，扫描配置（3,4）在扫描时将线形照明光束引导到探头f上，而在扫描结构 共焦孔被设计为开槽孔（26）或作为用作共焦孔的检测器单元（28）的槽形区域（28,48）。
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