Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents
    5.
    发明授权
    Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents 失效
    来自尖孢镰刀菌SPM 2672的内切蛋白酶用于洗涤剂

    公开(公告)号:US5288627A

    公开(公告)日:1994-02-22

    申请号:US977355

    申请日:1992-11-17

    摘要: An endoprotease preparation, comprising an isolated endoprotease is disclosed. The endoprotease is a serine protease, shows immunochemical idenity to a protease derived from Fusarium oxysporum DSM 2672, hydrolyzes the oxidized beta-chain of bovine insulin at the peptide bonds Arg (22)-Gly (23) and Lys (29)-Ala(30), has optimum activity towards casein in the pH range of 8.5-11.0 with nearly constant activity in the pH range, has optimum activity at a temperature of about 45.degree. C., and has an isolectric point of about 9.0-10.0. The preferred microorganism that the enzyme is isolated from is Fusarium oxysporum DSM 2672.

    摘要翻译: 公开了包含分离的内切蛋白酶的内切蛋白酶制剂。 内切蛋白酶是丝氨酸蛋白酶,显示出来自尖孢镰刀菌DSM 2672的蛋白酶的免疫化学鉴定性,在肽键Arg(22)-Gly(23)和Lys(29)-Ala(水解)时水解牛胰岛素的氧化的β-链 30),在pH范围为8.5-11.0的pH范围内具有最佳的酪蛋白活性,在pH范围内具有几乎恒定的活性,在约45℃的温度下具有最佳活性,并且具有约9.0-10.0的等电点。 酶分离的优选微生物是尖孢镰孢DSM 2672。

    Alkaline proteases obtainable from Bacillus sp. JA16-38A
    7.
    发明授权
    Alkaline proteases obtainable from Bacillus sp. JA16-38A 失效
    可从芽孢杆菌获得的碱性蛋白酶 JA16-38A

    公开(公告)号:US5650315A

    公开(公告)日:1997-07-22

    申请号:US107687

    申请日:1993-08-18

    摘要: An alkaline serine protease is disclosed which is obtainable from Bacillus sp. JA16-38A, NCIMB No. 40263 and which (a) has a pH optimum in the range of 9-11 determined at 25.degree. C.; (b) has a temperature optimum in the range of 40.degree.-50.degree. C. determined at pH 9.5; (c) is active in the presence of ethylene-diamine tetraacetate; and (d) has a mass of about 28 kD determined by SDS-PAGE. Detergent additives and detergent compositions comprising said protease and methods for producing said protease are also disclosed.

    摘要翻译: PCT No.PCT / DK92 / 00103 Sec。 371日期:1993年8月18日 102(e)日期1993年8月18日PCT提交1992年4月3日PCT公布。 第WO92 / 17576号公报 1992年10月15日的日期公开了一种碱性丝氨酸蛋白酶,其可从芽孢杆菌属 JA16-38A,NCIMB No.40263,其中(a)在25℃下的最佳pH为9-11。 (b)在pH9.5下测定温度最适于40°-50℃; (c)在乙二胺四乙酸酯存在下是有活性的; 和(d)通过SDS-PAGE测定的质量约为28kD。 还公开了包含所述蛋白酶的洗涤剂添加剂和洗涤剂组合物以及用于生产所述蛋白酶的方法。

    Nucleic acid constructs for the production of a Bacillus alkaline
protease
    9.
    发明授权
    Nucleic acid constructs for the production of a Bacillus alkaline protease 失效
    用于生产芽孢杆菌碱性蛋白酶的核酸构建体

    公开(公告)号:US5621089A

    公开(公告)日:1997-04-15

    申请号:US434255

    申请日:1995-05-03

    CPC分类号: C11D3/386 C12N15/75 C12N9/54

    摘要: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译: 本发明涉及分离的核酸构建体,其包含编码分子量为约34kD的碱性蛋白酶的核酸序列,并且可从芽孢杆菌属菌株获得。 组I,以及包含这种构建体的重组载体和宿主细胞。 另外,本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。

    Novel proteases
    10.
    发明授权
    Novel proteases 失效
    新型蛋白酶

    公开(公告)号:US5531918A

    公开(公告)日:1996-07-02

    申请号:US290932

    申请日:1994-08-22

    IPC分类号: C11D3/386 C12N9/54 C12R1/07

    CPC分类号: C12R1/07 C11D3/386 C12N9/54

    摘要: The present invention relates to proteases obtained from a strain of Bacillus sp. PD138, detergent additives and compositions comprising a Bacillus sp. PD138 protease, and methods of making and using a Bacillus sp. PD138 protease.

    摘要翻译: PCT No.PCT / DK93 / 00074 Sec。 371日期1994年8月22日 102(e)日期1994年8月22日PCT 1993年3月3日PCT公布。 出版物WO93 / 18140 日期:1993年9月16日。本发明涉及从芽孢杆菌属菌株获得的蛋白酶。 PD138,洗涤剂添加剂和包含芽孢杆菌属的组合物。 PD138蛋白酶,以及制备和使用芽孢杆菌属的方法。 PD138蛋白酶。