公开(公告)号：US06204023B1

公开(公告)日：2001-03-20

申请号：US08472691

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K39395

CPC分类号： C12N15/70 ,  A61K38/00 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30 ,  C12N9/88 ,  C12N15/81

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts tansformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有其的载体以及与其一起改造的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。本发明还涉及将编码果胶酶裂解酶信号肽的DNA克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜传输。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US5693493A

公开(公告)日：1997-12-02

申请号：US450731

申请日：1995-05-25

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

公开(公告)号：US5698435A

公开(公告)日：1997-12-16

申请号：US467140

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N5/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有相同载体的载体以及用其转化的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。 本发明还涉及编码果胶裂解酶信号肽的DNA已经克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜转运。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US5698417A

公开(公告)日：1997-12-16

申请号：US466203

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N5/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

公开(公告)号：US5618920A

公开(公告)日：1997-04-08

申请号：US235225

申请日：1994-04-29

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  A61K39/395 ,  C12N1/21 ,  C12N15/13

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to the secretion of heavy chain immunoglobulin fragments and light chain immunoglobulins from prokaryotic hosts using a prokaryotic secretion signal peptide wherein the heavy chain fragments and light chains are capable of associating to form an antigen binding antibody fragment.

摘要翻译： 本发明涉及使用原核分泌信号肽从原核宿主分泌重链免疫球蛋白片段和轻链免疫球蛋白，其中重链片段和轻链能够缔合形成抗原结合抗体片段。

公开(公告)号：US5595898A

公开(公告)日：1997-01-21

申请号：US299085

申请日：1994-08-18

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C07H21/04 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains.The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable-of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有相同载体的载体以及用其转化的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。 本发明还涉及编码果胶裂解酶信号肽的DNA已经克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜转运。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确折叠奇异果甜蛋白，并且抗体片段能够在靶癌细胞上结合抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US06461824B1

公开(公告)日：2002-10-08

申请号：US08467142

申请日：1995-06-06

申请人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung Paul Chang

发明人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung Paul Chang

IPC分类号： A61K39395

CPC分类号： C07K16/30 ,  A61K38/00 ,  A61K2039/505 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30 ,  C07K2319/33 ,  C07K2319/40 ,  G01N33/531 ,  G01N33/574 ,  G01N33/57492

摘要： The invention is directed to chimeric immunoglobulins that recognize a human tumor antigen bound by antibody, ING-2, ING-3, ING-4 or KM10, the production of such chimeric immunoglobulins, and their use.

摘要翻译： 本发明涉及识别由抗体ING-2，ING-3，ING-4或KM10结合的人肿瘤抗原的嵌合免疫球蛋白，这种嵌合免疫球蛋白的产生及其用途。

公开(公告)号：US5576184A

公开(公告)日：1996-11-19

申请号：US364001

申请日：1994-12-27

申请人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung P. Chang

发明人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung P. Chang

IPC分类号： A61K38/00 ,  C07K16/46 ,  G01N33/531 ,  G01N33/574 ,  G07K16/30

CPC分类号： C07K16/462 ,  G01N33/531 ,  G01N33/574 ,  G01N33/57492 ,  A61K2039/505 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  Y10S530/808

摘要： Gene expression elements and their use in production of chimeric antibodies with human constant regions and murine variable regions, and mouse human chimeric antibodies having specificity to human tumor cells, methods of their production, and their use.

摘要翻译： 基因表达元件及其在产生具有人恒定区和鼠可变区的嵌合抗体中的用途以及对人肿瘤细胞具有特异性的小鼠人嵌合抗体，其生产方法及其用途。

公开(公告)号：US5843685A

公开(公告)日：1998-12-01

申请号：US466034

申请日：1995-06-06

申请人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung Paul Chang

发明人： Marc D. Better ,  Arnold H. Horwitz ,  Randy R. Robinson ,  Shau-Ping Lei ,  Changtung Paul Chang

IPC分类号： A61K38/00 ,  C07K16/46 ,  G01N33/531 ,  G01N33/574 ,  A61K39/395 ,  C07K16/30

CPC分类号： C07K16/462 ,  G01N33/531 ,  G01N33/574 ,  G01N33/57492 ,  A61K2039/505 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  Y10S530/808

摘要： The invention is directed to a chimeric immunoglobulin that recognizes the human tumor antigen bound by antibody ING-1, its production, and use.

摘要翻译： 本发明涉及识别抗体ING-1结合的人肿瘤抗原及其生产和用途的嵌合免疫球蛋白。

公开(公告)号：US20120171226A1

公开(公告)日：2012-07-05

申请号：US13384197

申请日：2010-07-16

申请人： Arnold H. Horwitz

发明人： Arnold H. Horwitz

IPC分类号： A61K39/395 ,  G01N33/566 ,  C12N15/13 ,  C12N15/63 ,  C12N5/10 ,  A61P35/00 ,  C12N1/21 ,  C12N1/19 ,  C12N1/15 ,  C07K16/18 ,  C12P21/02

CPC分类号： C07K16/3053 ,  C07K2317/24

摘要： The present disclosure relates to anti-high molecular weight melanoma associated antigen (HMW-MAA) antibodies which bind to human HMW-MAA. Such antibodies may be used to treat diseases or disorders characterized by expression of HMW-MAA including cancer, for example, melanoma, basal cell carcinoma, ALL or AML.

摘要翻译： 本公开涉及结合人HMW-MAA的抗高分子量黑素瘤相关抗原（HMW-MAA）抗体。 这样的抗体可用于治疗特征在于包括癌症的HMW-MAA表达的疾病或病症，例如黑素瘤，基底细胞癌，ALL或AML。