公开(公告)号：US06204023B1

公开(公告)日：2001-03-20

申请号：US08472691

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K39395

CPC分类号： C12N15/70 ,  A61K38/00 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30 ,  C12N9/88 ,  C12N15/81

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts tansformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有其的载体以及与其一起改造的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。本发明还涉及将编码果胶酶裂解酶信号肽的DNA克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜传输。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US5693493A

公开(公告)日：1997-12-02

申请号：US450731

申请日：1995-05-25

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

公开(公告)号：US5698435A

公开(公告)日：1997-12-16

申请号：US467140

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N5/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有相同载体的载体以及用其转化的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。 本发明还涉及编码果胶裂解酶信号肽的DNA已经克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜转运。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US5698417A

公开(公告)日：1997-12-16

申请号：US466203

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N5/00 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

公开(公告)号：US5618920A

公开(公告)日：1997-04-08

申请号：US235225

申请日：1994-04-29

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  A61K39/395 ,  C12N1/21 ,  C12N15/13

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to the secretion of heavy chain immunoglobulin fragments and light chain immunoglobulins from prokaryotic hosts using a prokaryotic secretion signal peptide wherein the heavy chain fragments and light chains are capable of associating to form an antigen binding antibody fragment.

摘要翻译： 本发明涉及使用原核分泌信号肽从原核宿主分泌重链免疫球蛋白片段和轻链免疫球蛋白，其中重链片段和轻链能够缔合形成抗原结合抗体片段。

公开(公告)号：US5595898A

公开(公告)日：1997-01-21

申请号：US299085

申请日：1994-08-18

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C07H21/04 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts transformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains.The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable-of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

摘要翻译： 本发明涉及cDNA遗传序列，含有相同载体的载体以及用其转化的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。 本发明还涉及编码果胶裂解酶信号肽的DNA已经克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜转运。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确折叠奇异果甜蛋白，并且抗体片段能够在靶癌细胞上结合抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

公开(公告)号：US5846818A

公开(公告)日：1998-12-08

申请号：US472696

申请日：1995-06-06

申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horowitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horowitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

IPC分类号： A61K38/00 ,  C07K14/43 ,  C07K16/30 ,  C07K16/46 ,  C12N9/88 ,  C12N15/70 ,  C12N15/81 ,  C12N15/00 ,  C12N15/31 ,  C12N15/62 ,  C12P21/00

CPC分类号： C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C12N15/70 ,  C12N15/81 ,  C12N9/88 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30

摘要： The invention is directed to the pectate lyase B secretion signal and its use to express operably linked sequences in bacterial hosts.

摘要翻译： 本发明涉及果胶裂解酶B分泌信号及其在细菌宿主中表达可操作连接序列的用途。