公开(公告)号：USD297620S

公开(公告)日：1988-09-13

申请号：US658690

申请日：1984-10-09

申请人： Ray F. Cracauer ,  William R. Martin ,  William H. Harm

设计人： Ray F. Cracauer ,  William R. Martin ,  William H. Harm

公开(公告)号：US4804628A

公开(公告)日：1989-02-14

申请号：US88463

申请日：1987-08-19

申请人： Ray F. Cracauer ,  Robert D. Walker ,  Micheal L. Gruenberg

发明人： Ray F. Cracauer ,  Robert D. Walker ,  Micheal L. Gruenberg

IPC分类号： C12M1/12 ,  C12M3/06 ,  C12N5/02 ,  C12N5/00 ,  C02F1/44 ,  C12M1/14 ,  C12M3/00

CPC分类号： C12M41/40 ,  C12M25/10 ,  C12M29/16 ,  C12M29/18 ,  C12M41/00

摘要： An improved cell culturing device includes a hollow fiber cartridge having a shell and a plurality of capillaries extending through the shell with at least some of the capillaries having selectively permeable walls. A cell culturing space is located between the shell and capillaries. The improvement includes a chamber containing a second medium supply fluidly connected to the cell culturing space. A pressurizing system pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism alternatively and selectively restricts flow of medium between the chamber and the cell culturing space through first and second conduits such that circulation is effected in the cell culturing space.

摘要翻译： 一种改进的细胞培养装置包括具有壳体和多个毛细管的中空纤维筒，所述毛细管延伸穿过壳体，其中至少一些毛细管具有选择性渗透的壁。 细胞培养空间位于壳和毛细血管之间。 该改进包括包含流体连接到细胞培养空间的第二介质供应的腔室。 加压系统将腔室内的介质加压到高于流过毛细管内腔的介质水平的水平。 瓣膜机构可选地且选择性地通过第一和第二导管限制室和细胞培养空间之间的介质流动，使得在细胞培养空间中进行循环。