公开(公告)号：US20040062687A1

公开(公告)日：2004-04-01

申请号：US10287440

申请日：2002-11-01

Applicant: Rensselaer Polytechnic Institute

Inventor： Jonathan S. Dordick ,  Aravind Srinivasan ,  Jungbae Kim ,  David H. Sherman ,  Douglas S. Clark

IPC: B01J019/00

CPC classification number: B01J19/0093 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00691 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01J2219/00835 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0829

Abstract: Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.

Abstract translation: 本文公开了可用于制备天然产物及其类似物的微流体装置。 该装置包括固定在其上的生物合成途径的酶，以及以与生物合成途径的步骤相应的顺序将原料和每个随后的反应产物顺序地引导到生物合成途径的酶的装置。 因此，该装置可以用于使用天然起始物质的天然产物或天然产物的类似物使用非天然原料制备天然产物。 或者，可以通过以适当顺序的酶固定适当选择的酶在装置上来产生人工途径，其中每个随后的酶可以催化与先前酶的产物的反应。 这些人造途径可以制备新的化学物质。