公开(公告)号：US20040062687A1

公开(公告)日：2004-04-01

申请号：US10287440

申请日：2002-11-01

Applicant: Rensselaer Polytechnic Institute

Inventor： Jonathan S. Dordick ,  Aravind Srinivasan ,  Jungbae Kim ,  David H. Sherman ,  Douglas S. Clark

IPC: B01J019/00

CPC classification number: B01J19/0093 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00691 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01J2219/00835 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0829

Abstract: Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.

Abstract translation: 本文公开了可用于制备天然产物及其类似物的微流体装置。 该装置包括固定在其上的生物合成途径的酶，以及以与生物合成途径的步骤相应的顺序将原料和每个随后的反应产物顺序地引导到生物合成途径的酶的装置。 因此，该装置可以用于使用天然起始物质的天然产物或天然产物的类似物使用非天然原料制备天然产物。 或者，可以通过以适当顺序的酶固定适当选择的酶在装置上来产生人工途径，其中每个随后的酶可以催化与先前酶的产物的反应。 这些人造途径可以制备新的化学物质。

公开(公告)号：US20030166177A1

公开(公告)日：2003-09-04

申请号：US10287434

申请日：2002-11-01

Applicant: Rensselaer Polytechnic Institute

Inventor： Jonathan S. Dordick ,  Lakshmi Santhanam ,  Michael G. Hogg ,  James A. Holland

IPC: C12P007/00 ,  C08L071/10

CPC classification number: C12P13/001 ,  C12P7/24 ,  C12P7/26 ,  C12P11/00 ,  C12P13/00 ,  C12P13/002 ,  C12P13/008 ,  C12P13/02

Abstract: This invention relates to the enzymatic synthesis of oligophenols on solid support by sequential enzymatic addition of reaction solutions containing phenols. The oligomers are then selectively built up on the solid surface. When used in a specific format, the oligomers can be generated in a spatially addressable array, which can then be screened for some type of biological interaction. The synthetic compounds of the present invention are synthesized in a combinatorial manner on solid support using peroxidase or other related enzymatic catalysis, and the products are generated in spatially addressable microarrays. Oligophenols of the present invention have shown significance as potential inhibitors of NADPH oxidase assembly, an enzyme that has been implicated in a wide range of diseases stemming from vascular hyperpermeability.

Abstract translation: 本发明涉及通过依次酶加法加入含有酚的反应溶液来在固体支持物上酶促合成寡聚酚。 然后将低聚物选择性地构建在固体表面上。 当以特定格式使用时，可以在空间上可寻址的阵列中产生低聚物，其然后可以筛选某些类型的生物相互作用。 本发明的合成化合物以组合方式在使用过氧化物酶或其它相关酶促催化的固体支持物上合成，并且产物在空间可寻址的微阵列中产生。 本发明的寡聚物已经显示出作为潜在的NADPH氧化酶装配抑制剂的意义，NADPH氧化酶组装是涉及血管高渗透性的广泛疾病的酶。