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    Retroviral vector capable of transducing the aldehyde dehydrogenase-1 gene and making cells resistant to the chemotherapeutic agent cyclophosphamide and its derivatives and analogs
    1.
    发明授权
    Retroviral vector capable of transducing the aldehyde dehydrogenase-1 gene and making cells resistant to the chemotherapeutic agent cyclophosphamide and its derivatives and analogs 失效
    能够转导醛脱氢酶-1基因并使细胞对化学治疗剂环磷酰胺及其衍生物和类似物具有抗性的逆转录病毒载体

    公开(公告)号：US06268138B1

    公开(公告)日：2001-07-31

    申请号：US09221294

    申请日：1998-12-23

    申请人： Riccardo Dalla-Favera ,  Alessandro Massimo Gianni

    发明人： Riccardo Dalla-Favera ,  Alessandro Massimo Gianni

    IPC分类号： C12N900

    CPC分类号： C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2267/02 ,  C12N9/0008 ,  C12N9/93 ,  C12N15/86 ,  C12N2740/13043 ,  C12Y102/01003

    摘要： This invention provides viral and retroviral vectors which comprises a nucleic acid molecule encoding a human cytosolic aldehyde dehydrogenase or a glutamylcysteine synthetase or combinations thereof. Further, this invention provides an isolated mammalian nucleic acid molecule encoding an cytosolic aldehyde dehydrogenase and glutamylcysteine synthetase. In addition, this invention provides a method for reducing the toxic effects of a cyclophosphamide in a subject which comprises replacing the subject's hematopoietic cells with hematopoietic cells of having the retroviral vector. Further, this invention provides a method for introducing a selectable marker into a mammalian cell which comprises transfecting the cell with a nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase or glutamylcysteine synthetase. Lastly, this invention provides a method for selecting mammalian cells expressing protein of interest which comprises: a). introducing into the cells a nucleic acid molecule comprising a nucleic acid molecule encoding the protein of interest and the nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase; b.) culturing the resulting transfected cells; and c.) selecting cells which express human cytosolic aldehyde dehydrogenase.

    摘要翻译： 本发明提供病毒和逆转录病毒载体，其包含编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶的核酸分子或其组合。 此外，本发明提供了编码细胞溶质醛脱氢酶和谷氨酰半胱氨酸合成酶的分离的哺乳动物核酸分子。此外，本发明提供了降低受试者中环磷酰胺的毒性作用的方法，其包括用造血细胞代替受试者的造血细胞 此外，本发明提供了将选择标记引入哺乳动物细胞的方法，其包括用编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶的核酸分子转染细胞。最后，本发明提供了选择哺乳动物的方法 表达目的蛋白质的细胞，其包含：a）。 向细胞中引入包含编码目标蛋白的核酸分子和编码人胞浆醛脱氢酶的核酸分子的核酸分子; b）培养所得转染细胞; 和c）选择表达人胞浆醛脱氢酶的细胞。

    Retroviral vector capable of transducing the aldehyde dehydrogenase-1 gene and uses of said vector
    2.
    发明授权
    Retroviral vector capable of transducing the aldehyde dehydrogenase-1 gene and uses of said vector 失效
    能够转导醛脱氢酶-1基因的逆转录病毒载体和所述载体的用途

    公开(公告)号：US5888820A

    公开(公告)日：1999-03-30

    申请号：US347326

    申请日：1994-12-01

    申请人： Riccardo Dalla-Favera ,  Alessandro Massimo Gianni

    发明人： Riccardo Dalla-Favera ,  Alessandro Massimo Gianni

    IPC分类号： A01K67/027 ,  A61K31/70 ,  A61K39/395 ,  A61K48/00 ,  A61P43/00 ,  C07H21/04 ,  C07K16/40 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N15/09 ,  C12N15/85 ,  C12N15/867 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/536 ,  G01N33/573 ,  G01N33/577 ,  C12N15/00 ,  C12N15/63

    CPC分类号： C12N15/8509 ,  C12N15/86 ,  C12N9/0008 ,  C12N9/93 ,  C12Y102/01003 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2267/02 ,  C12N2740/13043

    摘要： This invention provides viral and retroviral vectors which comprises a nucleic acid molecule encoding a human cytosolic aldehyde dehydrogenase or a glutamylcysteine synthetase or combinations thereof. Further, this invention provides an isolated mammalian nucleic acid molecule encoding a cytosolic aldehyde dehydrogenase and glutamylecysteine synthetase. In addition, this invention provides a method for reducing the toxic effects of a cyclophosphamide in a subject which comprises replacing the subject's hematopoietic cells with hematopoietic cells having the retroviral vector. Further, this invention provides a method for introducing a selectable marker into a mammalian cell which comprises transfecting the cell with a nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase or glutamylcysteine synthetase. Lastly, this invention provides a method for selecting mammalian cells expressing protein of interest which comprises: a) introducing into the cells a nucleic acid molecule comprising a nucleic acid molecule encoding the protein of interest and the nucleic acid molecule encoding human cytosolic aldehyde dehdrogenase: b) culturing the resulting transfected cells; and c) selecting cells which express human cytosolic aldehyde dehdrogenase.

    摘要翻译： PCT No.PCT / US94 / 03624 Sec。 371 1994年12月1日第 102（e）日期1994年12月1日PCT 1994年4月1日PCT PCT。 WO94 / 23015 PCT公开 日期：1994年10月13日本发明提供包含编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶或其组合的核酸分子的病毒和逆转录病毒载体。 此外，本发明提供了编码胞质醛脱氢酶和谷氨酰半胱氨酸合成酶的分离的哺乳动物核酸分子。 此外，本发明提供减少受试者中环磷酰胺的毒性作用的方法，其包括用具有逆转录病毒载体的造血细胞代替受试者的造血细胞。 此外，本发明提供了将选择标记引入哺乳动物细胞的方法，其包括用编码人细胞溶质醛脱氢酶或谷氨酰半胱氨酸合成酶的核酸分子转染细胞。 最后，本发明提供了选择表达目的蛋白质的哺乳动物细胞的方法，其包括：a）将包含编码目标蛋白的核酸分子和编码人细胞溶质醛脱氢酶的核酸分子的核酸分子引入细胞：b ）培养所得转染细胞; 和c）选择表达人胞浆醛脱氢酶的细胞。