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公开(公告)号：US08216586B2
公开(公告)日：2012-07-10
申请号：US12453292
申请日：2009-05-06
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因本发明的特征在于新基因的全长核苷酸序列，cDNA序列还包含新型脂肪分解酶的全长编码序列 作为全长功能性蛋白质的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20090232936A1
公开(公告)日：2009-09-17
申请号：US12453292
申请日：2009-05-06
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因本发明的特征在于新基因的全长核苷酸序列，cDNA序列还包含新型脂肪分解酶的全长编码序列 作为全长功能性蛋白质的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US07550280B2
公开(公告)日：2009-06-23
申请号：US10524983
申请日：2003-08-15
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型脂肪分解酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20060281080A1
公开(公告)日：2006-12-14
申请号：US10524983
申请日：2003-08-15
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型脂肪分解酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20100047877A1
公开(公告)日：2010-02-25
申请号：US12461287
申请日：2009-08-06
申请人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
发明人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
CPC分类号： A21D8/042 , C07K2319/00 , C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码从黑曲霉分离的新型磷脂酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型磷脂酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的磷脂酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US07588925B2
公开(公告)日：2009-09-15
申请号：US11544679
申请日：2006-10-10
申请人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
发明人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
CPC分类号： A21D8/042 , C07K2319/00 , C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码从黑曲霉分离的新型磷脂酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型磷脂酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的磷脂酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US07838274B2
公开(公告)日：2010-11-23
申请号：US12461287
申请日：2009-08-06
申请人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
发明人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Bernhard Meima
CPC分类号： A21D8/042 , C07K2319/00 , C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码从黑曲霉分离的新型磷脂酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型磷脂酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的磷脂酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US20070207521A1
公开(公告)日：2007-09-06
申请号：US11544679
申请日：2006-10-10
申请人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Meima
发明人： Kaj Albermann , Wolfram Kemmner , Dieter Maier , Fabio Spreafico , Alexander Stock , Christian Wagner , Lex De Boer , Roelf Meima
CPC分类号： A21D8/042 , C07K2319/00 , C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码从黑曲霉分离的新型磷脂酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型磷脂酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的磷脂酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US07981636B2
公开(公告)日：2011-07-19
申请号：US11988365
申请日：2006-07-12
申请人： Holger Zorn , Manuela Scheibner , Bärbel Hülsdau , Ralf Günter Berger , Lex De Boer , Roelf Bernhard Meima
发明人： Holger Zorn , Manuela Scheibner , Bärbel Hülsdau , Ralf Günter Berger , Lex De Boer , Roelf Bernhard Meima
IPC分类号： C12P21/06
摘要： The present invention relates to novel polypeptides according to caroase 01-05 or any functional equivalents of any of them, suitable for use in a method for preparing a food products having increased whiteness, the use of the enzyme to increase whiteness of at least part of a food product, a process for preparing a food product wherein the enzyme is used and the food product obtained.
摘要翻译： 本发明涉及适合用于制备具有增加的白度的食品的方法中使用的根据caroase01-05的新型多肽或其中任何一种的任何功能等同物，该酶用于增加至少部分白蛋白的白度 食品，制备其中使用酶的食品的方法和获得的食品。
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公开(公告)号：US20110262974A1
公开(公告)日：2011-10-27
申请号：US13067476
申请日：2011-06-03
申请人： Holger Zorn , Manuela Scheibner , Bärbel Hulsdau , Ralf Günter Berger , Lex De Boer , Roelf Bernhard Meima
发明人： Holger Zorn , Manuela Scheibner , Bärbel Hulsdau , Ralf Günter Berger , Lex De Boer , Roelf Bernhard Meima
IPC分类号： C12N9/02 , C12N15/63 , C12N15/64 , D06L3/11 , C12N1/00 , C12N5/10 , A23L1/277 , C07H21/04 , C12P19/34
摘要： The present invention relates to novel polypeptides according to caroase 01-05 or any functional equivalents of any of them, suitable for use in a method for preparing a food products having increased whiteness, the use of the enzyme to increase whiteness of at least part of a food product, a process for preparing a food product wherein the enzyme is used and the food product obtained.
摘要翻译： 本发明涉及适合用于制备具有增加的白度的食品的方法中使用的根据caroase01-05的新型多肽或其中任何一种的任何功能等同物，该酶用于增加至少部分白蛋白的白度 食品，制备其中使用酶的食品的方法和获得的食品。
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