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    METHOD FOR THE DIAGNOSIS OF AGE-ASSOCIATED VASCULAR DISORDERS
    2.
    发明申请
    METHOD FOR THE DIAGNOSIS OF AGE-ASSOCIATED VASCULAR DISORDERS 审中-公开
    用于诊断与年龄相关的血管疾病的方法

    公开(公告)号：US20120004133A1

    公开(公告)日：2012-01-05

    申请号：US13202319

    申请日：2010-02-19

    申请人： Mingyi Wang ,  Zongming Fu ,  Edward G. Lakatta ,  Jennifer Van Eyk

    发明人： Mingyi Wang ,  Zongming Fu ,  Edward G. Lakatta ,  Jennifer Van Eyk

    IPC分类号： C40B30/04 ,  G01N33/53 ,  G01N27/447 ,  C12Q1/68

    CPC分类号： G01N33/6893 ,  G01N2333/485 ,  G01N2800/32 ,  G01N2800/323 ,  G01N2800/324 ,  G01N2800/325

    摘要： Methods for determining if a subject has or is susceptible to having an age-associated vascular disorder are disclosed. The method includes determining if the subject exhibits altered expression of a product of one or more of the genes listed in Table 1 relative to a control level of expression of the gene product. Altered expression of one or more of the genes listed in Table 1 indicates that the subject has or is susceptible to having an age-associated vascular disorder. In specific examples the gene product is a product of the MFG-E8 and an increase in expression indicates the subject has or is susceptible to having an age-associated vascular disorder.

    摘要翻译： 公开了确定受试者是否具有或具有年龄相关性血管疾病的方法。 该方法包括确定受试者是否表现出相对于基因产物的表达的对照水平，表1中列出的一种或多种基因的产物的表达改变。 表1中列出的一种或多种基因的改变的表达表明受试者具有或易患有年龄相关的血管疾病。 在具体实例中，基因产物是MFG-E8的产物，并且表达增加表示受试者具有或易于患有年龄相关的血管疾病。

    NUCLEIC ACID MODULES FOR EXPRESSION AND TAGGING OF MEMBRANE PROTEINS AND METHODS OF USE
    3.
    发明申请
    NUCLEIC ACID MODULES FOR EXPRESSION AND TAGGING OF MEMBRANE PROTEINS AND METHODS OF USE 失效
    用于表达和标记膜蛋白的核酸模块及其使用方法

    公开(公告)号：US20100173307A1

    公开(公告)日：2010-07-08

    申请号：US12652395

    申请日：2010-01-05

    申请人： Li Lin ,  John Pang ,  Wen Wei ,  Edward G. Lakatta

    发明人： Li Lin ,  John Pang ,  Wen Wei ,  Edward G. Lakatta

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/43 ,  C12N15/1086 ,  G01N33/542

    摘要： Described herein are nucleic acid modules for cloning, expression and tagging of eukaryotic membrane proteins. The nucleic acid modules include a receptor for advanced glycation end products (RAGE) signal sequence, a nucleic acid sequence encoding a tag and a multiple cloning sequence (MCS). Any membrane protein of interest can be cloned into the MCS for expression in cells. The nucleic acid modules can encode any type of tag, such as an epitope tag or affinity tag. The nucleic acid modules disclosed herein can be used to express any type of membrane protein and are particularly suited to the expression and tagging of Type I and Type III membrane proteins.

    摘要翻译： 本文描述了用于克隆，表达和标记真核细胞蛋白质的核酸模块。 核酸模块包括用于晚期糖基化终产物（RAGE）信号序列的受体，编码标签的核酸序列和多克隆序列（MCS）。 感兴趣的任何膜蛋白可以克隆到MCS中以在细胞中表达。 核酸模块可以编码任何类型的标签，例如表位标签或亲和标签。 本文公开的核酸模块可用于表达任何类型的膜蛋白，并且特别适用于I型和III型膜蛋白的表达和标记。

    Nucleic acid modules for expression and tagging of membrane proteins and methods of use
    5.
    发明授权
    Nucleic acid modules for expression and tagging of membrane proteins and methods of use 失效
    用于膜蛋白表达和标记的核酸模块及其使用方法

    公开(公告)号：US08501925B2

    公开(公告)日：2013-08-06

    申请号：US12652395

    申请日：2010-01-05

    申请人： Li Lin ,  John Pang ,  Wen Wei ,  Edward G. Lakatta

    发明人： Li Lin ,  John Pang ,  Wen Wei ,  Edward G. Lakatta

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/43 ,  C12N15/1086 ,  G01N33/542

    摘要： Described herein are nucleic acid modules for cloning, expression and tagging of eukaryotic membrane proteins. The nucleic acid modules include a receptor for advanced glycation end products (RAGE) signal sequence, a nucleic acid sequence encoding a tag and a multiple cloning sequence (MCS). Any membrane protein of interest can be cloned into the MCS for expression in cells. The nucleic acid modules can encode any type of tag, such as an epitope tag or affinity tag. The nucleic acid modules disclosed herein can be used to express any type of membrane protein and are particularly suited to the expression and tagging of Type I and Type III membrane proteins.

    摘要翻译： 本文描述了用于克隆，表达和标记真核细胞蛋白质的核酸模块。 核酸模块包括用于晚期糖基化终产物（RAGE）信号序列的受体，编码标签的核酸序列和多克隆序列（MCS）。 感兴趣的任何膜蛋白可以克隆到MCS中以在细胞中表达。 核酸模块可以编码任何类型的标签，例如表位标签或亲和标签。 本文公开的核酸模块可用于表达任何类型的膜蛋白，并且特别适用于I型和III型膜蛋白的表达和标记。

    Anti-marinobufagenin antibodies and methods for their use
    7.
    发明授权
    Anti-marinobufagenin antibodies and methods for their use 有权
    抗Marinobufagenin抗体及其使用方法

    公开(公告)号：US08038997B2

    公开(公告)日：2011-10-18

    申请号：US11993309

    申请日：2006-06-26

    申请人： Alexei Bagrov ,  Olga V. Fedorova ,  Edward G. Lakatta ,  Andrey Simbirtsev ,  Alexander Kotov ,  Nikolai Kolodkin

    发明人： Alexei Bagrov ,  Olga V. Fedorova ,  Edward G. Lakatta ,  Andrey Simbirtsev ,  Alexander Kotov ,  Nikolai Kolodkin

    IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K16/26 ,  G01N33/53

    CPC分类号： C07K16/26 ,  A61K2039/505 ,  C07K2317/76

    摘要： Described herein are deposited hybridoma cell lines and the monoclonal antibodies produced by these hybridomas, and antigen binding fragments thereof. These monoclonal antibodies and antigen binding fragments specifically bind marinobufagenin. The disclosure also encompasses the use of these monoclonal antibodies or antigen binding fragments in a method for detecting the presence of marinobufagenin in a biological sample. Also provided are methods for the use of these monoclonal antibodies or antigen binding fragments as prophylactic, therapeutic, and diagnostic agents for the detection, inhibition and treatment of a cardiovascular disease, for example, essential hypertension, hypertension associated with preeclampsia, eclampsia, or renal failure, or myocardial fibrosis in a subject.

    摘要翻译： 本文描述了沉积的杂交瘤细胞系和由这些杂交瘤产生的单克隆抗体及其抗原结合片段。 这些单克隆抗体和抗原结合片段特异性结合马氏原癌基因。 本公开还包括在用于检测生物样品中marinobufagenin的存在的方法中使用这些单克隆抗体或抗原结合片段。 还提供了将这些单克隆抗体或抗原结合片段用作用于检测，抑制和治疗心血管疾病的预防，治疗和诊断剂的方法，例如原发性高血压，与先兆子痫，子痫或肾脏相关的高血压 失败或心肌纤维化。

    ANTI-MARINOBUFAGENIN ANTIBODIES AND METHODS FOR THEIR USE
    8.
    发明申请
    ANTI-MARINOBUFAGENIN ANTIBODIES AND METHODS FOR THEIR USE 有权
    抗神经丝氨酸蛋白酶抗体及其使用方法

    公开(公告)号：US20100158900A1

    公开(公告)日：2010-06-24

    申请号：US11993309

    申请日：2006-06-26

    申请人： Alexei Bagrov ,  Olga V. Fedorova ,  Edward G. Lakatta ,  Andrey Simbirtsev ,  Alexander Kotov ,  Nikolai Kolodkin

    发明人： Alexei Bagrov ,  Olga V. Fedorova ,  Edward G. Lakatta ,  Andrey Simbirtsev ,  Alexander Kotov ,  Nikolai Kolodkin

    IPC分类号： C07K16/18 ,  C12N5/16 ,  C07K16/46 ,  G01N33/566 ,  A61K39/395 ,  A61P9/00 ,  A61P9/12 ,  A61P9/10

    CPC分类号： C07K16/26 ,  A61K2039/505 ,  C07K2317/76

    摘要： Described herein are deposited hybridoma cell lines and the monoclonal antibodies produced by these hybridomas, and antigen binding fragments thereof. These monoclonal antibodies and antigen binding fragments specifically bind marinobufagenin. The disclosure also encompasses the use of these monoclonal antibodies or antigen binding fragments in a method for detecting the presence of marinobufagenin in a biological sample. Also provided are methods for the use of these monoclonal antibodies or antigen binding fragments as prophylactic, therapeutic, and diagnostic agents for the detection, inhibition and treatment of a cardiovascular disease, for example, essential hypertension, hypertension associated with preeclampsia, eclampsia, or renal failure, or myocardial fibrosis in a subject.

    摘要翻译： 本文描述了沉积的杂交瘤细胞系和由这些杂交瘤产生的单克隆抗体及其抗原结合片段。 这些单克隆抗体和抗原结合片段特异性结合马氏原癌基因。 本公开还包括在用于检测生物样品中marinobufagenin的存在的方法中使用这些单克隆抗体或抗原结合片段。 还提供了将这些单克隆抗体或抗原结合片段用作用于检测，抑制和治疗心血管疾病的预防，治疗和诊断剂的方法，例如原发性高血压，与先兆子痫，子痫或肾脏相关的高血压 失败或心肌纤维化。