公开(公告)号：US06682648B1

公开(公告)日：2004-01-27

申请号：US09105538

申请日：1998-06-26

申请人： Robert D. MacPhee ,  Clive R. Taylor ,  Rainer Hintsche ,  Rene Seitz

发明人： Robert D. MacPhee ,  Clive R. Taylor ,  Rainer Hintsche ,  Rene Seitz

IPC分类号： G02N2726

CPC分类号： C12Q1/005 ,  C12Q1/6825 ,  G01N27/3277 ,  G01N33/5438

摘要： An immunochemical and molecular biologic endpoint reporter system in which reaction products, coupled to electrochemically active molecules susceptible to redox recycling or coupled to enzymes capable of proportional generation of said electrochemically active molecules, are detected and/or quantitated using amperiometry in conjunction with a silicon microchip possessing a closely spaced interdigitated array of nobel metal electrodes.

摘要翻译： 一种免疫化学和分子生物学终点报告系统，其中使用与硅微芯片结合的Amperionometry来检测和/或定量反应产物，其耦合到对氧化还原循环敏感的电化学活性分子或与能够成比例地产生所述电化学活性分子的酶偶联 具有紧密间隔的诺贝尔金属电极的交错排列。

公开(公告)号：US06881379B1

公开(公告)日：2005-04-19

申请号：US09958807

申请日：2000-04-14

申请人： Reinhard Bredehorst ,  Rainer Hintsche ,  Rene Seitz ,  Walter Gumbrecht

发明人： Reinhard Bredehorst ,  Rainer Hintsche ,  Rene Seitz ,  Walter Gumbrecht

IPC分类号： B01J19/00 ,  G01N33/50

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00704 ,  G01N27/27 ,  G01N27/3276

摘要： The invention relates to a method for producing a detection system for detecting different analytes in a sample, characterised by the following steps: providing a planar or essentially planar substrate which has sensors for chemically, optically or electrically detecting the analytes; applying an already microstructured layer to the substrate or applying a continuous layer to the substrate and microstructuring said layer, the layer being applied in such a way in either case that the areas of the substrate that are separated from each other are not covered by the layer, the layer and substrate being sealingly interconnected at least around the uncovered areas; bringing at least some of the uncovered areas into contact with at least one liquid containing catcher molecules, in such a way that said catcher molecules are able to adhere or bond to the surface of the substrate and/or on the surface of the sensors; removing the non-adhering constituents of the liquid and removing the microstructured layer or parts thereof.

摘要翻译： 本发明涉及一种生产用于检测样品中不同分析物的检测系统的方法，其特征在于以下步骤：提供具有用于化学，光学或电学检测分析物的传感器的平面或基本上平面的基底; 将已经微结构的层施加到基底上或向基底施加连续的层并且对所述层进行微结构化，所述层以这样的方式被施加，即在彼此分离的基底的区域不被层覆盖的情况下 所述层和衬底至少围绕所述未覆盖区域密封地互连; 使至少一些未覆盖区域与至少一种含捕获剂分子的液体接触，使得所述捕获剂分子能够粘附或结合到所述基底的表面和/或所述传感器的表面上; 去除液体的不粘附成分并除去微结构的层或其部分。