公开(公告)号：US06284126B1

公开(公告)日：2001-09-04

申请号：US09650025

申请日：2000-08-28

Applicant: Ronald T. Kurnik ,  Janet Tamada ,  Michael Tierney

Inventor： Ronald T. Kurnik ,  Janet Tamada ,  Michael Tierney

IPC: G02N2726

CPC classification number: A61B5/1486 ,  C12Q1/54

Abstract: An electrode assembly for sensing an electrochemical signal diffused from a source to a working electrode which is comprised of a plurality of substantially separated working electrode surfaces is disclosed. The electrode of the invention is comprised of 1) a working electrode made up of a plurality of working electrode surfaces or components and 2) a electrically insulating gap defined by adjacent edges of 1) insulating the working electrode surfaces or components from each other. The working electrode components are configured to receive electrochemical signal from two or preferably three dimensions simultaneously. The working electrode components configured over the same surface as a single electrode provide (1) an improved signal to noise ratio as compared to a single electrode by reducing noise, and (2) provide an overall enhanced signal after sensing for a given period of time.

Abstract translation: 公开了一种用于感测从源极扩散到工作电极的电化学信号的电极组件，其由多个基本上分离的工作电极表面组成。 本发明的电极包括1）由多个工作电极表面或部件组成的工作电极，以及2）由相邻边缘限定的电绝缘间隙，1）将工作电极表面或部件彼此绝缘。 工作电极部件被配置成从两个或优选三个维度同时接收电化学信号。 配置在与单个电极相同的表面上的工作电极组件提供（1）通过降低噪声与单个电极相比提高了信噪比，以及（2）在感测到给定时间段之后提供总体增强的信号 。

公开(公告)号：US06428668B1

公开(公告)日：2002-08-06

申请号：US09350090

申请日：1999-07-09

Applicant: Wilhelm Ansorge ,  Holger Erfle

Inventor： Wilhelm Ansorge ,  Holger Erfle

IPC: G02N2726

CPC classification number: G01N27/44743 ,  G01N33/54366 ,  G01N2035/1037

Abstract: In the method moving a sample substance by means of a sample feeder (26) form a sample pickup site to a delivery site (20) of a sample processor (10), the invention proposes fitting the sample feeder (26) with at least one porous material element having a pore size such that the sample substance shall be kept in the liquid phase in the porous material at least during sample pickup by the sample feeder (26) and during sample delivery to the sample processor (10).

Abstract translation: 在通过样品进料器（26）移动样品物质的方法中，将样品采集部位形成到样品处理器（10）的传送部位（20），本发明提出将样品进料器（26）装配至少一个 多孔材料元件具有使得样品物质至少在通过样品进料器（26）采集样品期间以及在样品传送到样品处理器（10）期间应保持在多孔材料中的液相中的孔径。

公开(公告)号：US06294062B1

公开(公告)日：2001-09-25

申请号：US09330422

申请日：1999-05-28

Applicant: Harvey B. Buck, Jr. ,  Zhi David Deng ,  Eric R. Diebold

Inventor： Harvey B. Buck, Jr. ,  Zhi David Deng ,  Eric R. Diebold

IPC: G02N2726

CPC classification number: C07F15/0026 ,  C07K5/101 ,  C07K7/02 ,  C07K7/06 ,  C07K9/001 ,  C07K14/805 ,  G01N27/3277 ,  G01N33/532 ,  G01N33/536 ,  G01N33/58 ,  G01N33/723 ,  G01N2333/805 ,  G01N2458/30 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T436/105831

Abstract: A method and device for detection and quantification of biologically significant analytes in a liquid sample is described. The method includes contacting a volume of a liquid sample with predetermined amounts of at least a first and second redox reversible species having redox potentials differing by at least 50 millivolts. At least one of the redox reversible species comprises a liquid sample diffusible conjugate of a ligand analog of an analyte in the liquid sample and a redox reversible label. A predetermined amount of at least one specific binding partner for each analyte to be measured is combined with the sample and current flow is measured at first and second anodic and cathodic potentials and correlated with current flows for known concentrations of the respective diffusible redox reversible species. Diagnostic devices and kits, including such devices and the specified specific binding partner(s) and redox reversible species are also described.

Abstract translation: 描述了用于在液体样品中检测和定量生物学显着性分析物的方法和装置。 该方法包括使体积的液体样品与预定量的至少50毫伏的氧化还原电位的至少第一和第二氧化还原可逆物质接触。 至少一种氧化还原可逆物质包括液体样品中分析物的配体类似物和氧化还原可逆标记的液体样品可扩散共轭物。 将待测量的每种待测量分析物的至少一个特定结合配偶体的预定量与样品组合，并且在第一和第二阳极和阴极电位处测量电流，并且与已知浓度的各个可扩散的氧化还原可逆物质的电流相关。 还描述了诊断装置和试剂盒，包括这些装置和指定的特异性结合配偶体和氧化还原可逆物质。

公开(公告)号：US06770179B1

公开(公告)日：2004-08-03

申请号：US09260007

申请日：1999-03-02

Applicant: Antonio Nanci

Inventor： Antonio Nanci

IPC: G02N2726

CPC classification number: B82Y30/00 ,  A61L27/28 ,  A61L27/34 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/254 ,  A61L2300/256 ,  A61L2300/258 ,  A61L2300/414 ,  A61L2300/64 ,  B82Y10/00 ,  B82Y15/00 ,  Y10S435/817

Abstract: The present invention relates to a biosensor which comprises a covalently attached monomolecular biological conjugate layer and a transducing system, wherein the biological conjugate layer has the following structural formula I: —R—X—P  I wherein, R is O or S covalently attached via a first covalent bond to the transducing device surface; X is a linker covalently attached to R via a second covalent bond and selected from a bond, linear or branched chains of 1 to 30 covalently attached atoms of at least C, N, O, Si or S, rings of at least one of C, N, O, Si or S, and a combination thereof; and P is a biological molecule stably attached to X via a third covalent bond.

Abstract translation: 本发明涉及包含共价连接的单分子生物共轭层和转导体系的生物传感器，其中生物共轭层具有以下结构式I：其中R是通过第一共价键与转导共价连接的O或S X是通过第二共价键共价连接到R的接头，并且选自至少C，N，O，Si或S的共价连接的原子的至少1至30个的键，直链或支链的至少一个 C，N，O，Si或S之一及其组合; 而P是通过第三共价键稳定地连接到X的生物分子。

公开(公告)号：US06375818B1

公开(公告)日：2002-04-23

申请号：US09328009

申请日：1999-06-08

Applicant: Tung-Liang Huang ,  M. Parameswara Reddy

Inventor： Tung-Liang Huang ,  M. Parameswara Reddy

IPC: G02N2726

CPC classification number: G01N27/44752 ,  Y10T428/1321

Abstract: An article of manufacture is provided that is useful in differentiating between solutes, such as during electrophoretic separations. An embodiment of the article is a capillary tube, that carries a polymer along the inner wall of the capillary tube. The polymer is effective to reduce undesired interactions and preferably includes a polylactam that is absorbed to the surface before the surface is exposed to the solutes. A preferred polylactam is poly(vinylpyrrolidone) with a molecular weight greater than about 1,000,000 daltons (weight-average).

Abstract translation: 提供了一种制造方法，其可用于区分溶质，例如在电泳分离期间。 制品的一个实施例是毛细管，其沿着毛细管的内壁承载聚合物。 聚合物有效减少不期望的相互作用，优选包括在表面暴露于溶质之前被吸收到表面的聚内酰胺。 优选的聚内酰胺是分子量大于约1,000,000道尔顿（重均分子）的聚（乙烯基吡咯烷酮）。

公开(公告)号：US06342143B1

公开(公告)日：2002-01-29

申请号：US09478664

申请日：2000-01-06

Applicant: Jonathan S. Minden

Inventor： Jonathan S. Minden

IPC: G02N2726

CPC classification number: G01N27/44739 ,  G01N2001/288

Abstract: The invention relates to a sample retrieval apparatus of particular benefit in the field of molecular biology. The apparatus permits the rapid collection of numerous specified fractions of samples: such as DNA, RNA or protein, that are separated by gel electrophoresis; or microorganisms grown on agar plates. The apparatus is capable of multiple sample retrieval from gels without cross-sample contamination from previously excised samples. Specifically, the sample retrieval apparatus is able to engage a cutting tip, cut a desired spot, band or plaque from a gel, deposit the desired band or plaque into a container, such as a multi-well plate for processing, and disengage the used cutting tip. The apparatus is then able to repeat this process many times to facilitate rapid and accurate processing of multiple bands or plaques from a single gel using different cutting tips for each sample retrieval.

Abstract translation: 本发明涉及在分子生物学领域特别有益的样品检索装置。 该装置允许快速收集通过凝胶电泳分离的许多指定级分的样品（例如DNA，RNA或蛋白质）; 或在琼脂平板上生长的微生物。 该装置能够从凝胶进行多次样品检索，而不会从先前切除的样品进行交叉样品污染。 具体地，样品回收装置能够接合切割尖端，从凝胶切割所需的点，带或斑块，将期望的带或斑块沉积到容器中，例如用于加工的多孔板，并且使所用的 切割尖端 该设备然后能够重复该过程多次，以促进从单个凝胶快速和准确地处理多个条带或斑块，使用每个样品检索的不同切割尖端。

公开(公告)号：US06328870B1

公开(公告)日：2001-12-11

申请号：US09491917

申请日：2000-01-27

Applicant: Richard Provonchee ,  Narendra Vartak

Inventor： Richard Provonchee ,  Narendra Vartak

IPC: G02N2726

CPC classification number: G01N27/44704

Abstract: A gel running plate for use in an electrophoresis process to cover an electrophoresis gel positioned within a tray. The gel running plate adapted to effect flow of electrical current into and out of the tray. The gel running plate having a length less than a length of the tray such that a gap exists between the tray and the plate when the plate is positioned on the tray. The gel running plate also having a width at least as wide as the width of the tray such that the plate rests upon the tray when the plate is positioned on the tray.

Abstract translation: 一种凝胶运行板，用于电泳过程中以覆盖位于托盘内的电泳凝胶。 凝胶运行板适于使电流流入和流出托盘。 凝胶流动板的长度小于托盘的长度，使得当板被定位在托盘上时，托盘和板之间存在间隙。 凝胶运行板还具有至少与托盘的宽度一样宽的宽度，使得当板被定位在托盘上时，板搁在托盘上。

公开(公告)号：US06464850B1

公开(公告)日：2002-10-15

申请号：US09363167

申请日：1999-07-29

Applicant: Tianhong Zhang ,  Noriko Kusukawa ,  Mark Garner

Inventor： Tianhong Zhang ,  Noriko Kusukawa ,  Mark Garner

IPC: G02N2726

CPC classification number: G01N27/44747

Abstract: The invention relates to a method for producing hydrophilic monomers which are particularly useful for electrophoresis and to electrophoresis compositions and coating compositions. The electrophoresis gel compositions and electrophoresis polymer compositions are hydrolytically stable and have high resolution. The method uses the steps of reacting a (meth)acryloyl with an aminoalcohol in the presence of a base in a polar solvent, optionally filtering an aqueous solution of the reaction product, deionizing an aqueous solution of the reaction product, and removing the solvent.

Abstract translation: 本发明涉及亲水性单体的制造方法，其特别适用于电泳和电泳组合物和涂料组合物。 电泳凝胶组合物和电泳聚合物组合物具有水解稳定性和高分辨率。 该方法使用以下步骤：在碱存在下使极性溶剂中的（甲基）丙烯酰基与氨基醇反应，任选地过滤反应产物的水溶液，使反应产物的水溶液去离子，并除去溶剂。

公开(公告)号：US06387232B1

公开(公告)日：2002-05-14

申请号：US09297744

申请日：1999-07-16

Applicant: Pankaj Maganlal Vadgama ,  Andrew David Maines

Inventor： Pankaj Maganlal Vadgama ,  Andrew David Maines

IPC: G02N2726

CPC classification number: C12Q1/002

Abstract: A membrane for use in a sensor having a detection arrangement. The membrane is of a cellulosic material incorporating a hydrophobic agent (e.g. isopropyl myristate) and is permeable by diffusion to an analyte species of interest to be detected directly or indirectly by the detection arrangement.

Abstract translation: 一种用于具有检测装置的传感器中的膜。 该膜是掺入疏水剂（例如肉豆蔻酸异丙酯）的纤维素材料，并且通过扩散到目标待分析物质可以渗透，以通过检测装置直接或间接检测。

公开(公告)号：US06383356B1

公开(公告)日：2002-05-07

申请号：US09448139

申请日：1999-11-24

Applicant: Yoshihide Hayashizaki ,  Shin Nakamura

Inventor： Yoshihide Hayashizaki ,  Shin Nakamura

IPC: G02N2726

CPC classification number: G01N27/44704

Abstract: A drain joint of a pump block is opened, a piston (19a) is pushed while a piston (13a) is fixed, for charging a passage between a Luer-Lok joint (17) and an intersection part as well as a passage between the intersection part and the drain joint with a buffer. Thereafter the piston (13a) is pushed while the piston (19a) is fixed, to charge a passage (7a) with a polymer. Then, the drain joint is closed, the piston (13a) is pushed and the piston (19a) is pulled in response to the amount of pushing, for charging the passage between the intersection point and the Luer-Lok joint (17) with the polymer. Thereafter, the piston (13a) is pushed while the piston (19a) is fixed, to charge a capillary column with the polymer.

Abstract translation: 打开泵块的排水接头，同时在固定活塞（13a）的同时推动活塞（19a），用于对路德洛接头（17）和交叉部分之间的通道以及通道 交叉部分和排水接头与缓冲器。 此后，在活塞（19a）被固定的同时推动活塞（13a），以便用聚合物对通道（7a）充电。 然后，关闭排水接头，推动活塞（13a），并且响应于推动量而拉动活塞（19a），用于对交点和鲁尔洛克接头（17）之间的通道进行充电， 聚合物。 此后，在固定活塞（19a）的同时推动活塞（13a），以便与聚合物一起为毛细管柱充电。