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公开(公告)号：US07259252B2
公开(公告)日：2007-08-21
申请号：US09974007
申请日：2001-10-10
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号：US06986989B2
公开(公告)日：2006-01-17
申请号：US09976863
申请日：2001-10-12
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
CPC分类号： C07H21/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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3.发明授权公开(公告)号：US06720147B2
公开(公告)日：2004-04-13
申请号：US09976577
申请日：2001-10-12
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
摘要翻译： 本发明提供了检测核酸的方法。 所述方法包括使核酸与一种或多种具有与其连接的寡核苷酸的颗粒接触。 在该方法的一个实施方案中，寡核苷酸与纳米颗粒连接并具有与核酸序列的部分互补的序列。 作为纳米颗粒上的寡核苷酸与核酸的杂交的结果，导致可检测的变化（优选变色）。 本发明还提供包含颗粒的组合物和试剂盒。 本发明还提供了合成独特的纳米颗粒 - 寡核苷酸缀合物的方法，通过该方法产生的缀合物和使用缀合物的方法。 此外，本发明提供纳米材料和包含纳米颗粒的纳米结构和使用纳米颗粒的纳米加工方法。 最后，本发明提供了从其他核酸中分离选择的核酸的方法。
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公开(公告)号：US06582921B2
公开(公告)日：2003-06-24
申请号：US09961949
申请日：2001-09-20
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
摘要翻译： 本发明提供了检测核酸的方法。 所述方法包括使核酸与一种或多种具有与其连接的寡核苷酸的颗粒接触。 在该方法的一个实施方案中，寡核苷酸与纳米颗粒连接并具有与核酸序列的部分互补的序列。 作为纳米颗粒上的寡核苷酸与核酸的杂交的结果，导致可检测的变化（优选变色）。 本发明还提供包含颗粒的组合物和试剂盒。 本发明还提供了合成独特的纳米颗粒 - 寡核苷酸缀合物的方法，通过该方法产生的缀合物和使用缀合物的方法。 此外，本发明提供纳米材料和包含纳米颗粒的纳米结构和使用纳米颗粒的纳米加工方法。 最后，本发明提供了从其他核酸中分离选择的核酸的方法。
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公开(公告)号：US06506564B1
公开(公告)日：2003-01-14
申请号：US09603830
申请日：2000-06-26
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C12Q168
CPC分类号： C07H21/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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6.发明授权公开(公告)号：US06417340B1
公开(公告)日：2002-07-09
申请号：US09693352
申请日：2000-10-20
IPC分类号： C07H1900
CPC分类号： C07H21/00 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6816 , C12Q1/6818 , C12Q1/6834 , C12Q1/6837 , C40B40/06 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/161 , C12Q2565/625 , C12Q2537/125
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing the nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
摘要翻译： 本发明提供了检测核酸的方法。 所述方法包括使核酸与一种或多种具有与其连接的寡核苷酸的颗粒接触。 在该方法的一个实施方案中，寡核苷酸与纳米颗粒连接并具有与核酸序列的部分互补的序列。 作为纳米颗粒上的寡核苷酸与核酸的杂交的结果，导致可检测的变化（优选变色）。 本发明还提供包含颗粒的组合物和试剂盒。 本发明进一步提供纳米材料和包含纳米颗粒的纳米结构和利用纳米颗粒的纳米加工方法。 最后，本发明提供了从其他核酸中分离选择的核酸的方法。
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公开(公告)号：US06361944B1
公开(公告)日：2002-03-26
申请号：US09344667
申请日：1999-06-25
IPC分类号： C12Q168
CPC分类号： C07H21/00 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6816 , C12Q1/6818 , C12Q1/6834 , C12Q1/6837 , C40B40/06 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/161 , C12Q2565/625 , C12Q2537/125
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing the nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号：US06984491B2
公开(公告)日：2006-01-10
申请号：US10008978
申请日：2001-12-07
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton , Viswanadham Garimella , Zhi Li , So-Jung Park , Gang Lu
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas Andrew Taton , Viswanadham Garimella , Zhi Li , So-Jung Park , Gang Lu
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2525/161 , C12Q2565/625 , C12Q2537/125 , C12Q2565/519 , C12Q2565/515
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号：US06861221B2
公开(公告)日：2005-03-01
申请号：US09975498
申请日：2001-10-11
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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公开(公告)号：US06828432B2
公开(公告)日：2004-12-07
申请号：US09975059
申请日：2001-10-11
申请人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C07H2100
CPC分类号： C07H21/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C07B2200/11 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
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