公开(公告)号：US5846395A

公开(公告)日：1998-12-08

申请号：US845425

申请日：1997-04-25

申请人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

发明人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

IPC分类号： G01N21/64 ,  C12Q1/48 ,  G01N27/447 ,  G01N35/10 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An electrophoresis apparatus for automatically performing medical assays includes an electrophoresis platform which cooperates with a gantry assembly. The electrophoresis platform and the gantry assembly are movable along paths that are perpendicular to each other. An applicator assembly includes pipettes which transfer fluid samples from a specimen tray to an electrophoresis plate mounted on the electrophoresis platform. The electrophoresis platform then moves to a position into the gantry assembly, where electrophoresis is conducted to separate the samples into different fractions. The electrophoresis platform then moves beneath a reagent pouring station where a reagent is applied to make the separated fractions fluoresce under ultraviolet light. The electrophoresis platform is then moved beneath the gantry assembly again, and an air knife in the gantry assembly spreads the reagent. After incubation and drying of the electrophoresis plate, the electrophoresis platform and gantry assembly are moved relative to one another while the electrophoresis plate is read with the aid of ultraviolet lamps and a photomultiplier tube mounted in the gantry assembly. The gain of the photomultiplier tube is automatically adjusted and the data gathered is automatically edited to remove background noise. The edited results can be printed or displayed on a video monitor. Techniques for calibrating the electrophoresis apparatus are also disclosed.

公开(公告)号：US5460709A

公开(公告)日：1995-10-24

申请号：US124502

申请日：1993-09-21

申请人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

发明人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

IPC分类号： G01N21/64 ,  C12Q1/48 ,  G01N27/447 ,  G01N35/10 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An electrophoresis apparatus for automatically performing medical assays includes an electrophoresis platform which cooperates with a gantry assembly. The electrophoresis platform and the gantry assembly are movable along paths that are perpendicular to each other. An applicator assembly includes pipettes which transfer fluid samples from a specimen tray to an electrophoresis plate mounted on the electrophoresis platform. The electrophoresis platform then moves to a position into the gantry assembly, where electrophoresis is conducted to separate the samples into different fractions. The electrophoresis platform then moves beneath a reagent pouring station where a reagent is applied to make the separated fractions fluoresce under ultraviolet light. The electrophoresis platform is then moved beneath the gantry assembly again, and an air knife in the gantry assembly spreads the reagent. After incubation and drying of the electrophoresis plate, the electrophoresis platform and gantry assembly are moved relative to one another while the electrophoresis plate is read with the aid of ultraviolet lamps and a photomultiplier tube mounted in the gantry assembly. The gain of the photomultiplier tube is automatically adjusted and the data gathered is automatically edited to remove background noise. The edited results can be printed or displayed on a video monitor. Techniques for calibrating the electrophoresis apparatus are also disclosed.

摘要翻译： 用于自动执行医学测定的电泳装置包括与台架组件配合的电泳平台。 电泳平台和龙门架组件可沿着彼此垂直的路径移动。 施用器组件包括移液管，其将流体样品从样品盘传送到安装在电泳平台上的电泳板。 电泳平台然后移动到龙门架组件的位置，其中进行电泳以将样品分离成不同的级分。 然后，电泳平台在试剂浇注站下方移动，其中施加试剂以使分离的部分在紫外光下发荧光。 然后将电泳平台再次移动到台架组件的下方，并且台架组件中的气刀将试剂扩散。 在电泳板的温育和干燥之后，电泳平台和龙门架组件相对于彼此移动，同时借助紫外线灯和安装在龙门架组件中的光电倍增管读取电泳板。 自动调整光电倍增管的增益，自动编辑收集的数据，消除背景噪音。 编辑结果可以打印或显示在视频监视器上。 还公开了用于校准电泳装置的技术。

公开(公告)号：US5516402A

公开(公告)日：1996-05-14

申请号：US478369

申请日：1995-06-07

申请人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

发明人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

IPC分类号： G01N21/64 ,  C12Q1/48 ,  G01N27/447 ,  G01N35/10 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An electrophoresis apparatus for automatically performing medical assays includes an electrophoresis platform which cooperates with a gantry assembly. The electrophoresis platform and the gantry assembly are movable along paths that are perpendicular to each other. An applicator assembly includes pipettes which transfer fluid samples from a specimen tray to an electrophoresis plate mounted on the electrophoresis platform. The electrophoresis platform then moves to a position into the gantry assembly, where electrophoresis is conducted to separate the samples into different fractions. The electrophoresis platform then moves beneath a reagent pouring station where a reagent is applied to make the separated fractions fluoresce under ultraviolet light. The electrophoresis platform is then moved beneath the gantry assembly again, and an air knife in the gantry assembly spreads the reagent. After incubation and drying of the electrophoresis plate, the electrophoresis platform and gantry assembly are moved relative to one another while the electrophoresis plate is read with the aid of ultraviolet lamps and a photomultiplier tube mounted in the gantry assembly. The gain of the photomultiplier tube is automatically adjusted and the data gathered is automatically edited to remove background noise. The edited results can be printed or displayed on a video monitor. Techniques for calibrating the electrophoresis apparatus are also disclosed.

公开(公告)号：US5583279A

公开(公告)日：1996-12-10

申请号：US478365

申请日：1995-06-07

申请人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

发明人： Robert J. Sarrine ,  Henry A. Garsee ,  Charles D. Kelley ,  Michael T. Everitt ,  Earl W. Boone ,  Philip A. Guadagno ,  Eric H. Petersen ,  Tipton L. Golias

IPC分类号： G01N21/64 ,  C12Q1/48 ,  G01N27/447 ,  G01N35/10 ,  G01F25/00 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An electrophoresis apparatus for automatically performing medical assays includes an electrophoresis platform which cooperates with a gantry assembly. The electrophoresis platform and the gantry assembly are movable along paths that are perpendicular to each other. An applicator assembly includes pipettes which transfer fluid samples from a specimen tray to an electrophoresis plate mounted on the electrophoresis platform. The electrophoresis platform then moves to a position into the gantry assembly, where electrophoresis is conducted to separate the samples into different fractions. The electrophoresis platform then moves beneath a reagent pouring station where a reagent is applied to make the separated fractions fluoresce under ultraviolet light. The electrophoresis platform is then moved beneath the gantry assembly again, and an air knife in the gantry assembly spreads the reagent. After incubation and drying of the electrophoresis plate, the electrophoresis platform and gantry assembly are moved relative to one another while the electrophoresis plate is read with the aid of ultraviolet lamps and a photomultiplier tube mounted in the gantry assembly. The gain of the photomultiplier tube is automatically adjusted and the data gathered is automatically edited to remove background noise. The edited results can be printed or displayed on a video monitor. Techniques for calibrating the electrophoresis apparatus are also disclosed.

摘要翻译： 用于自动执行医学测定的电泳装置包括与台架组件配合的电泳平台。 电泳平台和龙门架组件可沿着彼此垂直的路径移动。 施用器组件包括移液管，其将流体样品从样品盘传送到安装在电泳平台上的电泳板。 电泳平台然后移动到龙门架组件的位置，其中进行电泳以将样品分离成不同的级分。 然后，电泳平台在试剂浇注站下方移动，其中施加试剂以使分离的部分在紫外光下发荧光。 然后将电泳平台再次移动到台架组件的下方，并且台架组件中的气刀将试剂扩散。 在电泳板的温育和干燥之后，电泳平台和龙门架组件相对于彼此移动，同时借助紫外线灯和安装在龙门架组件中的光电倍增管读取电泳板。 自动调整光电倍增管的增益，自动编辑收集的数据，消除背景噪音。 编辑结果可以打印或显示在视频监视器上。 还公开了用于校准电泳装置的技术。

公开(公告)号：US06544395B1

公开(公告)日：2003-04-08

申请号：US09180320

申请日：1999-07-15

申请人： Mark E. Merchant ,  Philip A. Guadagno ,  Robert J. Sarrine ,  Suzan Robinson ,  James Robert Markus Sanford ,  Henry Garsee ,  Eric H. Petersen ,  Tipton L. Golias

发明人： Mark E. Merchant ,  Philip A. Guadagno ,  Robert J. Sarrine ,  Suzan Robinson ,  James Robert Markus Sanford ,  Henry Garsee ,  Eric H. Petersen ,  Tipton L. Golias

IPC分类号： G01N2726

CPC分类号： G01N27/44743 ,  Y10T436/2575

摘要： Novel apparatuses and methods for depositing or placing substances on substrates or plates are described. The invention relates to automated and semiautomated apparatuses and methods for controlled volume and precise placement of substances on substrates. Combinations of applicator tips, applicator tip assemblies, applicator reservoirs, applicator holders and movable racks precisely and accurately place samples and testing chemicals on substrates. Applicator tips particularly useful for dunk transfer and deposit processes and for carrying substances are disclosed. Apparatuses for precisely moving applicator holders, applicator reservoirs and tips are disclosed. The methods and apparatuses also have features for pre-loading substances on applicators and applicator reservoirs and precisely delivering the preloaded substances to substrates.

摘要翻译： 描述了用于将物质沉积或放置在基板或板上的新颖的装置和方法。 本发明涉及用于物质在基片上的受控体积和精确放置的自动化和半自动化设备和方法。 涂抹器尖端，涂抹器末端组件，涂敷器储存器，涂抹器支架和活动架的组合精确而准确地将样品和测试化学品放在基材上。 特别适用于扣篮转移和沉积过程以及携带物质的施用器技巧。 公开了用于精确移动施加器保持器，施加器容器和尖端的装置。 所述方法和装置还具有用于在施用器和施加器储存器上预加载物质并将预加载物质精确地递送到基底的特征。

公开(公告)号：US5512157A

公开(公告)日：1996-04-30

申请号：US361702

申请日：1994-12-22

申请人： Philip A. Guadagno ,  Rajani Rayachoti ,  Eric H. Petersen

发明人： Philip A. Guadagno ,  Rajani Rayachoti ,  Eric H. Petersen

IPC分类号： G02F1/167 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  G01N27/44747

摘要： An electrophoresis plate includes a substrate and an electrophoresis medium layer on the substrate. The electrophoresis plate may be used for medical testing by placing fluid specimens in wells on the electrophoresis medium layer so that the specimens can be separated into different fractions by applying an electric field across the electrophoresis medium layer. The electrophoresis medium layer includes liquid in a microporous gel such as agarose. The electrophoresis plate is packaged with a protective plastic film across the electrophoresis medium layer, but removal of the protective film prior to use of the electrophoresis plate leaves liquid that has been expressed from the electrophoresis medium layer on the surface of the electrophoresis medium layer. A surfactant such as methyl cellulose is included in the electrophoresis medium layer so that the expressed liquid will wet the electrophoresis medium layer in a uniform film instead of forming irregular pools or patches which would have to be removed before the electrophoresis plate is used.

摘要翻译： 电泳板包括基板和基板上的电泳介质层。 电泳板可用于医学测试，将液体样品放置在电泳介质层上，以便通过在电泳介质层上施加电场，将样品分离成不同的级分。 电泳介质层包括微孔凝胶如琼脂糖中的液体。 电泳板在电泳介质层上与保护性塑料膜一起包装，但是在使用电泳板之前除去保护膜会留下从电泳介质层表面的电泳介质层表达的液体。 表面活性剂如甲基纤维素被包括在电泳介质层中，使得表达的液体将以均匀的膜湿润电泳介质层，而不是在使用电泳板之前必须去除不规则的池或块。

公开(公告)号：US4741814A

公开(公告)日：1988-05-03

申请号：US14419

申请日：1987-02-12

申请人： David G. Mayes ,  James R. M. Sanford ,  Eric H. Petersen

发明人： David G. Mayes ,  James R. M. Sanford ,  Eric H. Petersen

IPC分类号： G01N27/447 ,  G01N27/26 ,  B65D6/00 ,  B65D85/30

CPC分类号： G01N27/44704

摘要： A container for protecting and enclosing an electrophoretic support medium is disclosed. The container includes a first portion and a second portion slidingly engageable with one another, whereby when closed they sealingly engage one another to form a substantially air-tight cavity therein. The first portion has a first recess defined by a protruding rim for accommodating a portion of the support medium therein. The second portion has a second recess defined by a protruding rim for accommodating the remainder of the support medium therein. The protruding rims are spaced apart from the bottoms of each portion and engagable with the base sheet of the support medium, whereby the support medium is retained within the recesses between the rims and the bottoms. The bottoms each have a substantially smooth planar surface to facilitate secure retention of the support medium.

摘要翻译： 公开了一种用于保护和封闭电泳支持介质的容器。 容器包括第一部分和第二部分，该第一部分和第二部分可滑动地彼此接合，由此当闭合时，它们彼此密封地接合以在其中形成基本上气密的空腔。 第一部分具有由突出边缘限定的第一凹部，用于将支撑介质的一部分容纳在其中。 第二部分具有由突出边缘限定的第二凹部，用于将支撑介质的其余部分容纳在其中。 突出的边缘与每个部分的底部间隔开并与支撑介质的基片接合，由此支撑介质保持在边缘和底部之间的凹槽内。 底部各自具有基本平滑的平坦表面，以便于支撑介质的牢固保持。

公开(公告)号：US4759838A

公开(公告)日：1988-07-26

申请号：US356

申请日：1987-01-05

申请人： David G. Mayes ,  James R. M. Sanford ,  Eric H. Petersen

发明人： David G. Mayes ,  James R. M. Sanford ,  Eric H. Petersen

IPC分类号： B65D43/16 ,  G01N27/447 ,  B65D6/00 ,  G01N27/26

CPC分类号： B65D43/162 ,  G01N27/44704 ,  B65D2251/1016

摘要： A container for protecting and enclosing an electrophoretic support medium is disclosed. The container includes a top portion and a bottom portion, which when closed sealingly engage one another to form a substantially air-tight cavity therein. The bottom portion has a recess formed therein for accommodating the support medium and at least a portion of the recess has a substantially smooth planar surface. The bottom recess is defined by a protruding continuous rim which has a plurality of nubs protruding into said recess and engagable with the base sheet of the support medium, whereby the support medium is retained within the recess.

摘要翻译： 公开了一种用于保护和封闭电泳支持介质的容器。 所述容器包括顶部和底部，当所述顶部和底部相互密封地彼此接合以在其中形成基本上气密的腔时。 底部具有形成在其中的用于容纳支撑介质的凹部，并且凹部的至少一部分具有基本平滑的平坦表面。 底部凹部由突出的连续边缘限定，突出的连续边缘具有突出到所述凹部中的多个凸起并且与支撑介质的基片接合，由此支撑介质保持在凹部内。