公开(公告)号：US06631289B2

公开(公告)日：2003-10-07

申请号：US09767125

申请日：2001-01-22

申请人： Robert R. Alfano ,  Jing Tang ,  Ping Pei Ho

发明人： Robert R. Alfano ,  Jing Tang ,  Ping Pei Ho

IPC分类号： A61B600

CPC分类号： G01N21/6486 ,  A61B5/0059 ,  A61B5/445 ,  A61B5/4523 ,  G01N21/6428 ,  G01N21/6456

摘要： A method for monitoring a biological tissue includes illuminating the tissue, including a fluorophore, with a wavelength of light, the wavelength selected for exciting the fluorophore, determining a fluorescent emission of the fluorophore, the emission indicating the presence of the fluorophore, and correlating an emission of the fluorophore to an extent and degree of damage to the tissue. Damage to the tissue includes a breakdown of the fluorophore, resulting in a reduced level of emission. The fluorophore can include one of collagen and elastin. The fluorophore can include tryptophan, nicotinamide adenine dinucleotide, flavin and porphyrin. Correlating the emission of the fluorophore to the extent and degree of damage further includes processing a correlation of the emission over time, controlling the power of a laser welder based on the processed correlation, and preventing overheating of the tissue by the laser welder.

公开(公告)号：US06208886B1

公开(公告)日：2001-03-27

申请号：US09054743

申请日：1998-04-03

申请人： Robert R. Alfano ,  Yici Guo ,  Feng Liu ,  Ping Pei Ho

发明人： Robert R. Alfano ,  Yici Guo ,  Feng Liu ,  Ping Pei Ho

IPC分类号： A61B500

CPC分类号： A61B5/0073 ,  A61B5/4519

摘要： An apparatus utilizing non-linear optical signals for use in constructing a three-dimensional tomographic map of an in vivo biological tissue for medical disease detection purposes. In one embodiment, said apparatus comprises a stage for supporting the in vivo biological tissue; a laser for illuminating the in vivo biological tissue with a focused beam of laser light, the light emerging from the in vivo biological tissue comprising fundamental light, harmonic wave light, and fluorescence due to multi-photon excitation; a filter for selectively passing only at least one of the harmonic wave light and the fluorescence; one or more detectors for individually detecting each of the harmonic wave light and the fluorescence selectively passed; and a mechanism for moving the laser relative to the stage in x, y and z directions.

摘要翻译： 一种利用非线性光信号用于构建用于医学疾病检测目的的体内生物组织的三维断层图的装置。 在一个实施方案中，所述装置包括用于支撑体内生物组织的载物台; 用聚焦光束照射体内生物组织的激光，从体内生物组织出射的光包括基本光，谐波光和由于多光子激发引起的荧光; 用于选择性地仅通过谐波光和荧光中的至少一个的滤光器; 一个或多个检测器，用于单独检测每个谐波光和选择性地通过的荧光; 以及用于在x，y和z方向上相对于舞台移动激光的机构。

公开(公告)号：US5717517A

公开(公告)日：1998-02-10

申请号：US386483

申请日：1995-02-10

申请人： Robert R. Alfano ,  Vladimir V. Petricevic ,  Ping Pei Ho

发明人： Robert R. Alfano ,  Vladimir V. Petricevic ,  Ping Pei Ho

IPC分类号： H01S3/067 ,  H01S3/16 ,  H01S3/17 ,  H01S3/00

CPC分类号： H01S3/06708 ,  H01S3/16 ,  H01S3/162 ,  H01S3/1623 ,  H01S3/178

摘要： A method for amplifying a signal pulse of laser light. Preferably, the method is used to amplify 1.3 .mu.m and/or 1.55 .mu.m signal pulses emitted from any 1.3 .mu.m or 1.55 .mu.m signal source, the method comprising providing an amplifying medium, the amplifying medium comprising an elongated core and a light-retaining outer structure surrounding the elongated core. The elongated core preferably comprises a plurality of Cr.sup.4+ -doped crystalline particles capable of lasing at 1.3 .mu.m and/or 1.55 .mu.m and preferably having a size of approximately 0.05 .mu.m to 500 .mu.m. The crystalline particles are dispersed within a non-gaseous medium, the non-gaseous medium having an index of refraction that substantially matches that of the crystalline particles. The method further comprises pumping the elongated core, either transversely or longitudinally, so as to excite the crystalline particles and transmitting the signal pulse of laser light through the elongated core while the crystalline particles are excited whereby the signal pulse of laser light becomes amplified. The present invention is also directed to an amplifier comprising the above-described amplifying medium in combination with a light source capable of pumping the elongated core in such a way as to excite the crystalline particles.

摘要翻译： 一种放大激光信号脉冲的方法。 优选地，该方法用于放大从任何1.3μm或1.55μm信号源发射的1.3μm和/或1.55μm信号脉冲，该方法包括提供放大介质，该放大介质包括细长芯和光 - 围绕细长核心的外部结构。 细长芯优选包括能够以1.3μm和/或1.55μm激发的多个Cr 4+掺杂的结晶颗粒，并且优选具有约0.05μm至500μm的尺寸。 结晶颗粒分散在非气体介质中，非气态介质的折射率基本上与结晶颗粒的折射率相匹配。 该方法还包括将细长的芯横向或纵向泵送，以便激发结晶颗粒并在激发结晶粒子的同时激发激光的信号脉冲通过细长芯，由此激光的信号脉冲变得放大。 本发明还涉及一种放大器，其包括上述放大介质与能够以激发结晶颗粒的方式泵送细长芯的光源。

公开(公告)号：US06240312B1

公开(公告)日：2001-05-29

申请号：US09178275

申请日：1998-10-23

申请人： Robert R. Alfano ,  Scott Alfano ,  Quan-Zhen Wang ,  Ping Pei Ho

发明人： Robert R. Alfano ,  Scott Alfano ,  Quan-Zhen Wang ,  Ping Pei Ho

IPC分类号： A61B600

CPC分类号： A61B1/041 ,  A61B1/00016 ,  A61B1/00027 ,  A61B1/00156 ,  A61B5/0031 ,  A61B5/07 ,  A61B34/35 ,  A61B34/72 ,  A61B2034/303 ,  A61B2562/028 ,  Y10S128/903

摘要： Remote-controllable, micro-scale, robotic device for use in diagnosing and/or treating abnormalities inside a human body in vivo. The device has a length from 0.1 mm to 10 mm and can be introduced into the body either from natural body openings or by injection into the blood stream. Once inside the body, the device can be guided to different locations in the body by an outside operator using radio controls and computer software. 2-dimensional image information and spectroscopic information (e.g., fluorescence, absorption, elastic scattering, Raman, etc.) gathered by the device inside the body are transmitted by video and radio signals to a computer located externally relative to the body. The transmitted information is processed, analyzed and displayed by the external computer for use by the outside operator. The outside operator can then make a diagnosis and, if applicable, instruct the device to render a treatment on the examined area. Such treatments include the ablation of tissue using lasers or the like and the binding of ruptured tissues together using chemical glue, UV cured epoxy materials or photochemical or photo-ionization techniques using near-infrared light to weld tissue from absorption at water bands.

摘要翻译： 用于诊断和/或治疗体内人体内异常的远程可控，微型，机器人装置。 该装置具有0.1mm至10mm的长度，并且可以从天然体开口或通过注入血流引入体内。 一旦进入体内，可以通过外部操作员使用无线电控制和计算机软件将装置引导到身体的不同位置。 通过视频和无线电信号将由身体内部的装置收集的2维图像信息和光谱信息（例如，荧光，吸收，弹性散射，拉曼等）传输到相对于身体外部的计算机。 所发送的信息由外部计算机处理，分析和显示以供外部操作者使用。 然后，外部操作人员进行诊断，如果适用，则指示设备对被检查区域进行处理。 这种治疗包括使用激光等消融组织，并使用化学胶，UV固化的环氧树脂材料或使用近红外光的光化学或光电离技术将破裂的组织结合在一起，以将组织从水带吸收焊接。