-
公开(公告)号：US5604108A
公开(公告)日：1997-02-18
申请号：US253251
申请日：1994-06-02
IPC分类号： A61K49/00 , G01N33/554
CPC分类号： A61K49/00
摘要： The dosage response of humans to conjugate vaccines comprising a bacterial polysaccharide and a strongly-immunogenic carrier protein is determined in animals by coadministering to the animal unconjugated strongly-immunogenic carrier protein in an amount corresponding, on a weight-related basis, to that administered to the human.
摘要翻译： 在动物中通过将与重量相关的量对应于动物未缀合的强免疫原性载体蛋白的动物来测定人类对包含细菌多糖和强免疫原性载体蛋白的结合疫苗的剂量反应。 人类。
-
公开(公告)号：US5985288A
公开(公告)日：1999-11-16
申请号：US472172
申请日：1995-06-07
申请人： Robert S. Munson, Jr. , Susan Grass , Pele Chong , Yan-Ping Yang , Raafat Fahim , Dwo Yan Charles Sia , Patrick McVerry , Michel Klein
发明人： Robert S. Munson, Jr. , Susan Grass , Pele Chong , Yan-Ping Yang , Raafat Fahim , Dwo Yan Charles Sia , Patrick McVerry , Michel Klein
IPC分类号： A61K39/00 , A61K39/102 , A61K39/385 , A61P31/04 , A61P31/12 , A61P31/16 , C07K14/005 , C07K14/195 , C07K14/285 , C07K14/41 , C07K14/705 , C07K19/00 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , C12R1/21 , G01N33/569
CPC分类号： C07K14/285 , A61K39/00
摘要： The gene for outer membrane protein P1 of Haemophilus influenzae b is expressed in E. coli. Methods for expression and demonstration of the immunogenicity of recombinant P1 and portions thereof are disclosed, along with an improved method for the purification of P1. The nucleotide sequence of the P1 gene and the derived amino acid sequence of the P1 protein of Haemophilus influenzae type b are disclosed and the methods used to determine the same. Also disclosed are the methods used to clone and express the P1 gene as well as the purification protocol for the P1 gene products (recombinant P1 and P1 fusion proteins). Fourteen peptides are synthesized corresponding to specific sequences of the mature P1 protein. The use of the P1 protein as n immunogens for immunization against the disease caused by Haemophilus influenzae type b and the use of the protein as a carrier for conjugation with an oligosaccharide derived from Haemophilus to generate a potentially efficacious vaccine against the disease, are described. Also disclosed is the use of P1 peptide-conjugates as immunizing agents to elicit anti-Haemophilus influenzae type b antibodies.
摘要翻译： 流感嗜血杆菌b的外膜蛋白P1的基因在大肠杆菌中表达。 公开了用于表达和证明重组P1及其部分的免疫原性的方法以及用于纯化P1的改进方法。 公开了P1型基因的核苷酸序列和B型流感嗜血杆菌的P1蛋白的衍生氨基酸序列，并且用于确定它们的方法。 还公开了用于克隆和表达P1基因的方法以及P1基因产物（重组P1和P1融合蛋白）的纯化方案。 对应于成熟P1蛋白的特异性序列合成14个肽。 描述了使用P1蛋白作为免疫原免疫针对由流感嗜血杆菌b型引起的疾病的免疫，以及使用蛋白质作为与源自嗜血杆菌的寡糖缀合的载体以产生针对该疾病的潜在有效疫苗。 还公开了使用P1肽缀合物作为免疫剂以引发抗流感嗜血杆菌b型抗体。
-
公开(公告)号：US5916562A
公开(公告)日：1999-06-29
申请号：US123245
申请日：1993-09-20
申请人： Robert S. Munson, Jr. , Robert Tolan , Pele Chong , Raafat Fahim , Patrick McVerry , Michel Klein
发明人： Robert S. Munson, Jr. , Robert Tolan , Pele Chong , Raafat Fahim , Patrick McVerry , Michel Klein
IPC分类号： A61K39/00 , A61P31/04 , C07K14/285 , A61K39/02
CPC分类号： C07K14/285 , A61K39/00
摘要： The nucleotide sequence of the P2 gene and the derived amino acid sequence of the P2 protein of Haemophilus influenzae type b are disclosed and the methods used to determine the same. Also disclosed are the methods used to clone and express the P2 gene as well as the purification protocol for the gene and protein. Also disclosed is the synthesis of peptides corresponding to the N-terminal and C-terminal ends of the P2 protein. Also disclosed is the use of the P2 protein as a protective agent for the disease caused by Haemophilus influenzae type b and the use of the protein as a carrier for conjugation with an oligosaccharide derived from Haemophilus to generate a potentially efficacious vaccine against the disease. Also disclosed is the use of P2 peptide-conjugates as immunising agents.
摘要翻译： 公开了P2型基因的核苷酸序列和流感嗜血杆菌b型的P2蛋白的衍生氨基酸序列，并且用于确定其的方法。 还公开了用于克隆和表达P2基因的方法以及用于基因和蛋白质的纯化方案。 还公开了对应于P2蛋白的N末端和C末端的肽的合成。 还公开了P2蛋白作为由流感嗜血杆菌b型引起的疾病的保护剂的用途，以及使用该蛋白质作为与衍生自嗜血杆菌的寡糖缀合的载体以产生针对该疾病的潜在有效的疫苗。 还公开了使用P2肽缀合物作为免疫剂。
-
-
搜索结果
3 条记录 (耗时 0.019 秒)
