公开(公告)号：US20210207122A1

公开(公告)日：2021-07-08

申请号：US15777577

申请日：2016-11-21

申请人： Robi David Mitra ,  Jeffrey Milbrandt ,  Ezra S. Abrams ,  Todd J. Barbera ,  T. Christian Boles ,  SAGE SCIENCE, INC. ,  WASHINGTON UNIVERSITY

发明人： Robi David MITRA ,  Jeffrey MILBRANDT ,  Ezra Solomon ABRAMS ,  Todd J. BARBERA ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447

摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

公开(公告)号：US20170240882A1

公开(公告)日：2017-08-24

申请号：US15519516

申请日：2015-10-15

申请人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES ,  SAGE SCIENCE, INC.

发明人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447 ,  C12Q1/68

CPC分类号： C12N15/101 ,  C12M47/06 ,  C12N15/1017 ,  C12Q1/6806 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44782

摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

公开(公告)号：US20230227808A1

公开(公告)日：2023-07-20

申请号：US18149603

申请日：2023-01-03

申请人： Sage Science, Inc. ,  Washington University

发明人： Robi David Mitra ,  Jeffrey MILBRANDT ,  Ezra Solomon ABRAMS ,  Todd J. BARBERA ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447

CPC分类号： C12N15/101 ,  G01N27/44747

摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase re-agents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

公开(公告)号：US20190153426A1

公开(公告)日：2019-05-23

申请号：US16192520

申请日：2018-11-15

申请人： SAGE SCIENCE, INC.

发明人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447 ,  C12Q1/6806

摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

公开(公告)号：US20200041449A1

公开(公告)日：2020-02-06

申请号：US16339648

申请日：2017-10-04

申请人： Sage Science, Inc.

发明人： Ezra Solomon ABRAMS ,  Todd J. BARBERA

IPC分类号： G01N27/447 ,  B01J19/00 ,  C12N15/10

摘要： Various implementations disclosed herein relate to automated processing of nucleic acids and electrophoretic sample preparation. An exemplary disposable cassette for automated molecular processing may include a base housing, a central channel arranged in the housing, and an elution module configured to be received in central channel and to divide the central channel into a first chamber and a second chamber. The elution module comprises a housing having a proximal side, a distal side and an elution module channel passing from the proximal side to the distal side. The elution module also comprises a first membrane attached to a proximal side of the elution module, a second membrane attached to a distal side of the elution module, and a porthole in fluid communication with the elution module channel and configured for receiving a sample.

公开(公告)号：US20210062180A1

公开(公告)日：2021-03-04

申请号：US16959989

申请日：2019-01-04

申请人： Sage Science, Inc.

发明人： Ezra ABRAMS ,  Todd J. BARBERA ,  Chris BOLES

IPC分类号： C12N15/10 ,  G01N27/447 ,  B01L3/00

摘要： A cassette for retaining molecules during electrophoresis has a housing with a lane configured therein. The lane has a first elongate edge and a second elongate edge, and an elution module is configured to be received in the late to divide the lane into a first chamber and a second chamber. A first buffer reservoir is positioned adjacent the first elongate edge, and a second buffer reservoir is positioned adjacent the second elongate edge. The first side of the elution module facing the first chamber comprises a porous sterile filtration membrane. The second side of the elution module facing the second chamber comprises an ultrafiltration membrane that has a pore size to retain molecules during electrophoresis.