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公开(公告)号：US07452668B2
公开(公告)日：2008-11-18
申请号：US10888767
申请日：2004-07-09
CPC分类号： C12Q1/6816 , G01N27/44726 , G01N2035/1053
摘要： Methods of detecting target molecules using electrophoresis and media containing immobilized capture are described.
摘要翻译： 描述了使用电泳检测靶分子的方法和含有固定捕获的培养基。
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2.发明申请公开(公告)号：US20170239658A1
公开(公告)日：2017-08-24
申请号：US15519515
申请日：2015-10-16
申请人： Ezra S. ABRAMS , Christian T. BOLES , Yu CHEN , James STURM , Robert AUSTIN , SAGE SCIENCE, INC. , TRUSTEES OF PRINCETON UNIVERSITY
发明人： Ezra S. ABRAMS , T. Christian BOLES , Yu CHEN , James STURM , Robert AUSTIN
IPC分类号： B01L3/00 , G01N27/447 , G01N15/14 , C12Q1/68
CPC分类号： B01L3/502761 , B01L2200/0652 , B01L2200/0663 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , G01N15/1484 , G01N27/44791 , G01N2015/1006 , G01N2015/149 , G01N2015/1493 , G01N2015/1497 , C12Q2523/308 , C12Q2565/629
摘要： In some embodiments, a method for manipulating DNA molecules for use in a microfluidic device is provided, where the method may comprise providing a solution of a plurality of DNA molecules having a first radius of gyration under under a zero flow velocity, and maintaining the DNA molecules in a spherical shape under a flow velocity.
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公开(公告)号：US20090152116A1
公开(公告)日：2009-06-18
申请号：US12251190
申请日：2008-10-14
CPC分类号： C12Q1/6816 , G01N27/44726 , G01N2035/1053
摘要： Methods of detecting target molecules using electrophoresis and media containing immobilized capture are described.
摘要翻译： 描述了使用电泳检测靶分子的方法和含有固定捕获的培养基。
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4.发明授权Multiple sequential polynucleotide displacement reactions for signal amplification and processing 有权用于信号放大和处理的多序列多核苷酸置换反应公开(公告)号：US06255051B1
公开(公告)日：2001-07-03
申请号：US09188086
申请日：1998-11-06
IPC分类号： C07H2104
CPC分类号： C12Q1/6823 , C12Q1/6813 , C12Q1/682 , C12Q1/6827
摘要： Methods for determining the presence of a target polynucleotide sequence using chemical hybridization in sequential probe and displacement complex formation with potential for signal gain prior to detection are disclosed.
摘要翻译： 公开了使用化学杂交确定目标多核苷酸序列存在的方法，所述化合物序列探针和位移复合物形成与检测前具有信号增益的潜力。
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5.发明申请公开(公告)号：US20170240882A1
公开(公告)日：2017-08-24
申请号：US15519516
申请日：2015-10-15
申请人： Ezra S. ABRAMS , Danny YUN , Todd J. BARBERA , Douglas Grosvenor SABIN , T. Christian BOLES , SAGE SCIENCE, INC.
IPC分类号： C12N15/10 , G01N27/447 , C12Q1/68
CPC分类号： C12N15/101 , C12M47/06 , C12N15/1017 , C12Q1/6806 , G01N27/44747 , G01N27/44773 , G01N27/44782
摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.
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公开(公告)号：US06238927B1
公开(公告)日：2001-05-29
申请号：US09411777
申请日：1999-10-04
申请人： Ezra S. Abrams , Philip W. Hammond
发明人： Ezra S. Abrams , Philip W. Hammond
IPC分类号： G01N3300
CPC分类号： C12Q1/6823 , Y10T436/143333 , C12Q2537/149 , C12Q2537/1373 , C12Q2525/101 , C12Q2565/628 , C12Q2565/125 , C12Q2565/518 , C12Q2565/627 , C12Q2565/501
摘要： The present invention pertains to a method and compositions for detecting the presence of a target nucleic acid in a test sample in which the sample to be tested for the presence of the target nucleic acid is introduced into a solution containing the probe-tether complex under conditions suitable for hybridization to occur between the first probe nucleic acid sequence and the target nucleic acid sequence so that the second tether nucleic acid sequence is displaced from the probe-tether complex, and a new complex is formed by the hybridization of the first probe nucleic acid sequence and the target nucleic acid sequence
摘要翻译： 本发明涉及用于检测试验样品中目标核酸存在的方法和组合物，其中将待测试的靶核酸的存在样品在条件下导入含有探针 - 系链复合物的溶液中 适合于在第一探针核酸序列和靶核酸序列之间发生杂交，使得第二系链核酸序列从探针 - 系链复合物中移位，并且通过第一探针核酸的杂交形成新的复合物 序列和靶核酸序列
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公开(公告)号：US20210207122A1
公开(公告)日：2021-07-08
申请号：US15777577
申请日：2016-11-21
申请人： Robi David Mitra , Jeffrey Milbrandt , Ezra S. Abrams , Todd J. Barbera , T. Christian Boles , SAGE SCIENCE, INC. , WASHINGTON UNIVERSITY
发明人： Robi David MITRA , Jeffrey MILBRANDT , Ezra Solomon ABRAMS , Todd J. BARBERA , T. Christian BOLES
IPC分类号： C12N15/10 , G01N27/447
摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.
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公开(公告)号：US06492118B1
公开(公告)日：2002-12-10
申请号：US09649637
申请日：2000-08-28
IPC分类号： C12Q168
CPC分类号： C07H21/04 , C12Q1/6834 , C12Q1/6837 , G01N33/54353 , C12Q2523/107
摘要： A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.
摘要翻译： 提供了将配体例如核酸固定在固体支持物上的方法。 该方法包括提供含有固定化的潜在硫醇基团的固体支持物，活化硫醇基团，使活化的硫醇基团与包含丙烯酰胺官能团的核酸接触，并在两组之间形成共价键，从而将核酸固定在 坚实的支持。 还提供了包含固体支持物和使用固体支持物的方法的试剂盒。
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公开(公告)号：US06300070B1
公开(公告)日：2001-10-09
申请号：US09327083
申请日：1999-06-04
申请人： T. Christian Boles , Ezra S. Abrams
发明人： T. Christian Boles , Ezra S. Abrams
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/686 , C12Q2565/518
摘要： A multi-stage bridge amplification method which uses a recovered single-stranded amplification single-stranded nucleic acid molecule to initiate second and subsequent stages of bridge amplification is described.
摘要翻译： 描述了使用回收的单链扩增单链核酸分子启动桥联扩增的第二和后续阶段的多级桥扩增方法。
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